We narrowed to 9,389 results for: Coli

1. pY1

   Plasmid

   #50597

   Purpose

   Backbone: pRBS-01, Origin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5,

   Depositor

   Jay Keasling

   Article

   Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

   Type

   Empty backbone

   Use

   Synthetic Biology; Empty vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLacUV5

   Available since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pIIS18-SapI

   Plasmid

   #128599

   Purpose

   Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

   Depositor

   Tohru Suzuki

   Article

   Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRSET FLIPglu-2uDelta13

   Plasmid

   #13561

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

   Insert

   FLIPglu F16F D152A Delta13
   

   Use

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   F16A, D152A in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pIIS18-BsmBI

   Plasmid

   #128648

   Purpose

   Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

   Depositor

   Tohru Suzuki

   Article

   Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLBC0150

   Plasmid

   #172795

   Purpose

   Level 0 module containing NT2_TurboID

   Depositor

   Benjamin Field

   Article

   Velay et al Plant Methods. 2022 May 26;18(1):69. doi

   Insert

   TurboID with 3XHA tag
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   no

   Promoter

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHCKan-yibDp-GST

   Plasmid

   #134592

   Purpose

   Low Phosphate inducible GST

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   Glutathione S-transferase
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Promoter

   yibD

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Vh-HA-DHFR

   Plasmid

   #20217

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   DHFR (folA E. coli)
   

   Use

   Tags

   HA and Vh

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pPJ173(JPUB_006777)

   Plasmid

   #83834

   Purpose

   Carries NEW operon 10 final

   Depositor

   Harry Beller

   Article

   Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

   Inserts

   Kuste3336
   Kuste3339
   Kuste3341
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   op10_3339_for, op10_3341_for, and op10_BsaI_3336_…

   Available since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPJ167(JPUB_006765)

   Plasmid

   #83831

   Purpose

   Carries NEW operon 3 final

   Depositor

   Harry Beller

   Article

   Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

   Inserts

   Kuste2803
   Kuste2804
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   op3_2804_for and op3_BsmBI_2803_for

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKB237

    Plasmid

    #170030

    Purpose

    Encodes LacI-controlled expression of surface-displayed CCL19 and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    CCL19 (CCL19 Synthetic, Escherichia coli, Human)
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTU1-A-lacZ

    Plasmid

    #72935

    Purpose

    Level 1 Destination vector - Position A

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 1 - A site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Beta-lactamase - G717C (nucleotide) - silent muta…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSB74

    Plasmid

    #19591

    Depositor

    Jay Keasling

    Article

    Bang et al Appl Environ Microbiol. 2000 Sep . 66(9)

    Insert

    thiosulfate reductase (phsB S. enterica serovar Typhimurium LT2)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pDY4

    Plasmid

    #53589

    Purpose

    Produces an active Lactuca sativa (lettuce) lycopene epsilon-cyclase in E. coli. Use with pAC-LYC or pAC-LYCipi to produce epsilon-carotene in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Proc Natl Acad Sci U S A. 2001 Feb 27;98

    Insert

    lcyE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCx55.5-TrpR

    Plasmid

    #86373

    Purpose

    for zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherry

    Depositor

    Rachel Wong

    Article

    Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

    Insert

    TrpR
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    cx55.5

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-clbB

    Plasmid

    #114155

    Purpose

    Expresses full-length ClbB

    Depositor

    Emily Balskus

    Article

    Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

    Insert

    clbB
    

    Use

    Tags

    6xHis-thrombin-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBEL2003

    Plasmid

    #139888

    Purpose

    Expression plasmid for LetAB with 5 ring (rings 12347) LetB (without TM region) mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; delta515-746)-6xHis

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBEL2000

    Plasmid

    #139885

    Purpose

    Expression plasmid for LetAB with 2 ring (rings17) LetB (without TM region) mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; delta161-747)-6xHis

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXylA-agrCA-IV

    Plasmid

    #53438

    Purpose

    xylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureus

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    agrCA locus
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Bacterial

    Mutation

    "MVQTS" sequence added to the N-terminu…

    Promoter

    PxylA

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMMB206-CT61

    Plasmid

    #165410

    Purpose

    Bacteria gene circuit on low copy backbone.

    Depositor

    Xiaojun Tian

    Article

    Zhang et al Nat Commun. 2021 Feb 8;12(1):853. doi: 1

    Insert

    CT61 gene circuit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSET FLII32Pglu-2.2m

    Plasmid

    #13566

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16A P32S-ECFP-D33R
    

    Use

    Tags

    6xHis and Citrine

    Expression

    Bacterial

    Mutation

    F16A, ECFP insert in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 3xDHFR-myc

    Plasmid

    #20216

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    3xDHFR (folA E. coli)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

22. MazF-K56A

    Plasmid

    #164274

    Purpose

    To express recombinant MazF K56A point mutant protein

    Depositor

    Guanzheng Luo

    Article

    Zhang et al Sci Adv. 2019 Jul 3;5(7):eaax0250. doi:

    Insert

    MazF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K56A

    Promoter

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCDF-yibDp-matB

    Plasmid

    #134597

    Purpose

    Low Phosphate inducible matB (malonyl-CoA synthetase)

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    Malonyl CoA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibD

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. SepOTSγ

    Plasmid

    #68284

    Purpose

    Phosphoserine orthogonal translation system

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    SepRS9/EF-Sep21, 1xtRNA-Sep
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBP-SJM911

    Plasmid

    #72973

    Purpose

    Level 0 - SJM911 promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    SJM911 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

26. Ppj217(JBEI-13454)

    Plasmid

    #89787

    Purpose

    for expression of N-8xHis-StrepII-MBP-3607

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Insert

    3607
    

    Use

    Tags

    8xHis-StrepII-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SepOTSε

    Plasmid

    #68286

    Purpose

    Phosphoserine orthogonal translation system

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    SepRS9/EF-Sep21, 1xtRNA-Sep-A37
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPZP FLIPmal-25u

    Plasmid

    #14476

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPmal
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    W230A

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

29. mNG-mCh

    Plasmid

    #124215

    Purpose

    Cytoplasmic mNeongreen mCherry tandem, ~19 % energy transfer pNM002 - pSAV057-mNG-mCh

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al MBio. 2017 Sep 12;8(5). pii: mBio.01089-

    Insert

    mNG-mCh
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SepOTSθ

    Plasmid

    #68289

    Purpose

    Phosphoserine orthogonal translation system

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    SepRS/EF-Sep, 2xtRNA-Sep-A37
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBEST-p15A-J23101-deGFP-T500 (131p)

    Plasmid

    #70207

    Purpose

    Promoter panel

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSAH0016

    Plasmid

    #79824

    Purpose

    Dual selection plasmid with lac operator, selection for binding with spectinomycin, for non-binding with chloramphenicol

    Depositor

    Katja Arndt

    Article

    Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

    Inserts

    cat
    aadA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    AATTCAAAAAAATGCTTGACAATTAATCATCGGCTCGTATAATGTGTGG…

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Vh-HA-3xDHFR

    Plasmid

    #20219

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    3xDHFR (folA E. coli)
    

    Use

    Tags

    HA and Vh

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

34. pRSET FLII12Pglu-600uDelta4

    Plasmid

    #13567

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    FLIPglu F16A Y12S-ECFP-D13R, 4aa linker
    

    Use

    Tags

    Citrine

    Expression

    Bacterial

    Mutation

    F16A, ECFP insert in mglB

    Promoter

    Available since

    Feb. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 2xDHFR-myc

    Plasmid

    #20215

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    2xDHFR (folA E. coli)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

36. pTU2_2a

    Plasmid

    #73010

    Purpose

    Secondary module (BpiI) site in pTU2a backbone

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Secondary module + Golden Gate pTU2a BsmBI destination site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28a-clbH

    Plasmid

    #114156

    Purpose

    Expresses full-length ClbH

    Depositor

    Emily Balskus

    Article

    Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

    Insert

    clbH
    

    Use

    Tags

    6xHis-thrombin-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFP140

    Plasmid

    #21672

    Depositor

    James Haber

    Article

    Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

    Insert

    Lacz (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Tags

    Expression

    Mutation

    The second lacZ sequence has been modified, the E…

    Promoter

    Available since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

39. pFP120

    Plasmid

    #21673

    Depositor

    James Haber

    Article

    Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

    Insert

    LacZ (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Tags

    Expression

    Mutation

    The second lacZ sequence has been modified, a 918…

    Promoter

    Available since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

40. pTU1-B-lacZ

    Plasmid

    #72936

    Purpose

    Level 1 Destination vector - Position B

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 1 - B site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Beta-lactamase - G717C (nucleotide) - silent muta…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge