We narrowed to 8,039 results for: Dos;
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Plasmid#119622PurposeLevel 0 Part. CDSDepositorInsertdCas9+deg-tag
UseSynthetic BiologyTagsExpressionMutationbp 331 C to A , 1237 A to C, 1284 C to T, 2121 C …PromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.055
Plasmid#119566PurposeLevel 0 part. PromoterDepositorInsertPisiAB
UseSynthetic BiologyTagsExpressionMutationbp 391 G to A, RBS* added (22 bp) upstream of ATG…PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-GFP-Atg17(416) (I325D)
Plasmid#121412PurposeExpressing GFP-Atg17DepositorInsertCUP1p-GFP-ATG17 ORF-CYC1 terminator
UseTagsExpressionYeastMutationIsoleucine 325 to Aspartic acidPromoterCUP1 promoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-GFP-Atg17(416) (F317D)
Plasmid#121411PurposeExpressing GFP-Atg17DepositorInsertCUP1p-GFP-ATG17 ORF-CYC1 terminator
UseTagsExpressionYeastMutationPhenylalanine 317 to Aspartic acidPromoterCUP1 promoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-GFP-Atg17(416) (L105D)
Plasmid#121410PurposeExpressing GFP-Atg17DepositorInsertCUP1p-GFP-ATG17 ORF-CYC1 terminator
UseTagsExpressionYeastMutationLeucine 105 to Aspartic acidPromoterCUP1 promoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(L313D I314K)
Plasmid#121390PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationLeucine 313 to Aspartic acid; Isoleucine 314 to L…PromoterADH1 promoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-Atg17(416) (L105D)
Plasmid#121405PurposeExpressing Atg17DepositorInsertCUP1p-ATG17 ORF-CYC1 terminator
UseTagsExpressionYeastMutationLeucine 105 to Aspartic acidPromoterCUP1 promoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUPER.retro.puro-shLARP7
Plasmid#113537PurposeRetroviral vector designed to knock down LARP7.DepositorInsertshLARP7 (LARP7 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1_MePCE_aa432-689
Plasmid#113543PurposeProduces an N-terminal GST-tagged MePCE protein consisting of amino acids 432 - 689.DepositorInsertMePCE (MEPCE Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1_MePCE_aa400-689
Plasmid#113542PurposeProduces an N-terminal GST-tagged MePCE protein consisting of amino acids 400 - 689.DepositorInsertMePCE (MEPCE Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1_MePCE_aa1-400
Plasmid#113541PurposeProduces an N-terminal GST-tagged MePCE protein consisting of amino acids 1 - 400.DepositorInsertMePCE (MEPCE Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#493
Plasmid#110932PurposeExpresses proteins in C. elegans cholinergic neurons in head, ventral nerve cord, and tailDepositorInsertsunc-17 promoter
unc-54 3' control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#94
Plasmid#110931PurposeExpresses proteins in C. elegans cholinergic neurons in head, ventral nerve cord, and tailDepositorInsertsunc-17 promoter
unc-54 3' control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-CenpB 1-166
Plasmid#108474Purposeexpression of vsv-CenpB 1-166DepositorInsertCenpB 1-166 AA (CENPB Human)
UseTagsVSVExpressionMammalianMutationencodes only the DNA-binding domain of CENP-BPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-Mis12-dCEN-Incenp
Plasmid#108477Purposeexpression of vsv-Mis12-dCEN-INCENPDepositorInsertMis12-dCEN-INCENP (INCENP Human)
UseTagsVSVExpressionMammalianMutationCEN box replaced by Mis12PromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-vsv-Mis12-dCEN-Incenp-EGFP
Plasmid#108478Purposeexpression of vsv-Mis12-dCEN-INCENP-EGFPDepositorInsertMis12-dCEN-INCENP (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationCEN box replaced by Mis12PromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_N_Strep
Plasmid#101651PurposeExpression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cellsDepositorInsertLRP6 Signal peptide, KpnI linker, Twin-Strep tag
UseTagsTwin-Strep tagExpressionInsectMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_C_Strep
Plasmid#101652PurposeExpression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cellsDepositorInsertLRP6 Signal peptide, MCS linker, Twin-Strep tag
UseTagsTwin-Strep tagExpressionInsectMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_C_3xFLAG
Plasmid#101653PurposeExpression of type I signal-peptide containing proteins with C-terminal 3xFLAG-tag in insect cellsDepositorInsertLRP6 Signal peptide, MCS linker, 3xFLAG tag
UseTags3xFLAG tagExpressionInsectMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL2-MCT2Prom2.5
Plasmid#87351PurposeHuman monocarboxylate transporter 2 (MCT2) promoter 2.5 kbpDepositorInsertMCT2 (SLC16A7 Human)
UseLuciferaseTagsExpressionBacterial and MammalianMutationPromoter2.5 kbp proximal promoter of human MCT2 (nested d…Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL2-MCT2Prom1.1
Plasmid#87352PurposeHuman monocarboxylate transporter 2 (MCT2) promoter 1.1 kbpDepositorInsertMCT2 (SLC16A7 Human)
UseLuciferaseTagsExpressionBacterial and MammalianMutationPromoter1.1 kbp proximal promoter of human MCT2 (nested d…Available sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL2-MCT2Prom3.2
Plasmid#87349PurposeHuman monocarboxylate transporter 2 (MCT2) promoter 3.2 kbpDepositorInsertMCT2 (SLC16A7 Human)
UseLuciferaseTagsExpressionBacterial and MammalianMutationPromoter3.2 kbp proximal promoter of MCT2 (nested deletio…Available sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX335_HR_Prnp_3
Plasmid#78622PurposepX335 vector encoding SpCas9n and a chimeric guide RNA targeting Prnp coding sequenceDepositorInsertHR_Prnp_3 (Prnp Mouse)
UseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterU6Available sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
sgRNA(MS2)_Prnp_SAM2
Plasmid#78625PurposeEncodes sgRNA2.0 targeting 138nt upstream of TSSDepositorInsertsgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
sgRNA(MS2)_Prnp_SAM1
Plasmid#78624PurposeEncodes sgRNA2.0 targeting 158nt upstream of TSSDepositorInsertsgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB421
Plasmid#68686PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pClneoEGFPC-let60 K16N
Plasmid#66861PurposeExpress GFP-tagged dominat negative let60, in which Lysine 16 is mutated to asparagine, in mammalian cellsDepositorInsertLet-60 (let-60 Nematode)
UseTagsGFPExpressionMammalianMutationLysine 16 is mutated to asparaginePromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-Cx43-FS
Plasmid#65440PurposeCloning, T7 RNA expressionDepositorInsertCx43 (GJA1 Human)
UseT7 in vitro rna productionTagsExpressionBacterialMutationFrameshift (FS) mutation 35delAPromoterT7Available sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB2a Cterminal tail C127S, E435C
Plasmid#60398PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) C127S, E435C for crosslinking polyglutamate peptideDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB4a Cterminal tail C127S, E435C
Plasmid#60405PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) C127S, E435C for crosslinking polyglutamate peptideDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-PIM2mut3
Plasmid#53742Purposepromoter region of PIM2 lacking the 2 forkhead binding sitesDepositorInsertPIM2 (PIM2 Human)
UseLuciferase; Reporter vectorTagsExpressionMutationdeletion of 2 forkhead binding sites (bp# 395-497…PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSMP-MBD4_3
Plasmid#36376DepositorInsertMBD4 (MBD4 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSMP-MBD4_2
Plasmid#36375DepositorInsertMBD4 (MBD4 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pZT-C13-R1
Plasmid#62197PurposeChr.13/CLYBL TALEN expression vectorDepositorInsertRight CLYBL TALEN
UseTALENTagsExpressionMutationPromoterCMVAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZT-C13-L1
Plasmid#62196PurposeChr.13/CLYBL TALEN expression vectorDepositorInsertLeft CLYBL TALEN
UseTALENTagsExpressionMutationPromoterCMVAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
HsTPC2-EYFP
Plasmid#135194PurposeExpresses tagged TPC2 in mammalian cellsDepositorInsertTPC2 (TPCN2 Human)
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_MDM2_SWIB
Plasmid#178481PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertMDM2_SWIB (MDM2 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Frankenbody-FLAG-SNAP
Plasmid#175295PurposeTrack mature FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with SNAP-tag
UseTagsSNAP-tagExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-Frankenbody-FLAG-mEGFP
Plasmid#175293PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with mEGFP
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV/D377Y-mPCSK9
Plasmid#58376PurposeExpresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosisDepositorInsertproprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
UseAAVTagsExpressionMammalianMutationD377YPromoterHCRApoE/hAATAvailable sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Frankenbody-FLAG-mRuby2
Plasmid#175294PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with mRuby2
UseTagsmRuby2ExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLJC6-3XHA-TMEM192
Plasmid#104434PurposeExpresses 3XHA-TMEM192 in mammalian cellsDepositorInsertTMEM192 (TMEM192 Human)
UseLentiviralTags3XHAExpressionMammalianMutationPromoterUbiquitinAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK172-α-syn-TEV-GFP
Plasmid#166671PurposeBacterial expression of alpha synuclein coupled to GFP via a TEV site for uptake studiesDepositorInsertmouse synuclein (from V. Lee) (Snca Mouse)
UseTagsGFPExpressionBacterialMutationadded a TEV cleavage site sequence between synucl…PromoterCMVAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide_DKO
Plasmid#183193PurposePlasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screenDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6, mU6Available sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAS:hGCG-ICAM(1235)- Nrxn1b;cryaa:mCherry
Plasmid#218860Purpose10xUAS promoter driving expression of human glucagon-truncated ICAM-zfNrxn1b fusion protein for use in trans-Tango.DepositorInserthGCG-ICAM(1235)-nrxn1b
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICE-FLAG-NAT10-siR-WT
Plasmid#59365PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInserthuman NAT10 (NAT10 Human)
UseTagsFLAGExpressionMammalianMutationMutations to render cDNA resistant to a siRNAPromoterCMV-tetAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-NLS-Cas9-6xHis
Plasmid#62934PurposeExpression of NLS-Cas9-6xHis in bacterial cellsDepositorInsertNLS-Cas9-6xHis
UseTags6xHis tag and NLSExpressionBacterialMutationPromoterT7Available sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)
Plasmid#101139PurposeLuciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3 GCN1-3xFlag_IRES-iRFP
Plasmid#198388PurposeGCN1 expressionDepositorInsertGCN1 (GCN1 Human)
UseLentiviralTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR_DKO
Plasmid#183192PurposePlasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screenDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6, mU6Available sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only