Addgene

We narrowed to 6,341 results for: Mag

Showing: 2581 - 2610 of 6341 results

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pBAD-GINKO1

Plasmid

#113111

Purpose

Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

GINKO1

Use

Tags

6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pRcCMV myc-Plk1 K82R (Nigg LA184)

Plasmid

#41157

Depositor

Erich Nigg

Article

Smits et al Nat Cell Biol. 2000 Sep;2(9):672-6.

Insert

Plk1 (PLK1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

K82R

Promoter

CMV

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only
XMRV vp62

Plasmid

#26189

Depositor

Joseph DeRisi

Article

Urisman et al PLoS Pathog. 2006 Mar . 2(3):e25.

Insert

XMRV VP62 (gag Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2010

Availability

Academic Institutions and Nonprofits only
pLVX-EF1a-PTDSS1-IRES-Puromycin

Plasmid

#134667

Purpose

Expresses EGFP-tagged Mitochondria-associated ER membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

PTDSS1 (PTDSS1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFU-MVIIA-PC

Plasmid

#24148

Depositor

Ines Ibanez-Tallon

Article

Auer et al Nat Methods. 2010 Feb 7. ():.

Insert

omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-mCherry (PDGFRB Conus magus, Aequorea victoria, Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2010

Availability

Academic Institutions and Nonprofits only

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VV229: GCaMP6f in fck

Plasmid

#58514

Purpose

Expresses GCaMP6f under the a-CamKII promoter

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

GCaMP6f

Use

Lentiviral

Tags

C-terminal FCYENEV (ER export motif); this was no…

Expression

Mammalian

Mutation

Promoter

a-CamKII

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pFH2.107_PB-EPN24-MCP_p6-T2A-eGFP-PGK-Blast

Plasmid

#205552

Purpose

Express EPN24-MCP-p6-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN24-MCP-p6

Use

Piggybac

Tags

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-mNINJ1-GFP

Plasmid

#208776

Purpose

MSCV vector expressing WT mouse NINJ1

Depositor

Petr Broz

Article

Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

Insert

NINJ1 (Ninj1 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mutation

Promoter

Available since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mClover3-10aa-H2B

Plasmid

#74257

Purpose

Mammalian expression of N-terminal fusion of mClover3 to H2B

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

H2B

Use

Tags

mClover3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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PL-SIN-EOS-C(3+)-EGFP

Plasmid

#21318

Purpose

Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EOS-C(3+) promoter region

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pmScarlet_NES_C1

Plasmid

#85060

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

NES

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pmScarlet-I_peroxisome_C1

Plasmid

#85065

Purpose

In vivo visualization of the peroxisome (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Peroxisomal targeting sequence

Use

Tags

mScarlet-i

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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dClover2-C1

Plasmid

#54577

Purpose

Localization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.

Depositor

Michael Davidson

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Type

Empty backbone

Use

Tags

dClover2

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV.TBG mCherry-LDTS-LIRmut

Plasmid

#189003

Purpose

lipophagy-inducing adaptor protein, AAV, negative control

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

LDTS-LIRmut, mCherry

Use

AAV

Tags

HA

Expression

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Prab-3::NLS::GCaMP6s

Plasmid

#68119

Purpose

Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

rab-3::NLS::GCaMP6s

Use

Tags

Expression

Worm

Mutation

Promoter

rab-3

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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mEmerald-MAP4-C-10

Plasmid

#54152

Purpose

Localization: Microtubules, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

MAP4

Use

Tags

mEmerald

Expression

Mammalian

Mutation

aa 640-1045 of MAP4

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pAG1280 NLS-mCherry-FIREtag-NES

Plasmid

#205172

Purpose

expresses NLS-mCherry-FIREtag-NES in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

NLS-mCherry-FIREtag-NES

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-YFP-SLBP(18-126)-IRES-H1-Skylan62A

Plasmid

#205760

Purpose

Single-color FUCCI4 sensor for S/G2 cell cycle transition and G2/M transition

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

YFP-SLBP(18-126)-IRES-H1-Skylan62A

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGEM-sgMUC1_dual-MTS-3x-Set I

Plasmid

#162763

Purpose

Separately expressing 3 unique sgMUC1_dual-MTS (SetI)

Depositor

Antony K Chen

Article

Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

Insert

sgMUC1_dual-MTS_1; sgMUC1_dual-MTS_2; sgMUC1_dual-MTS_3

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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BRAC/pcDNA3

Plasmid

#51967

Purpose

mammalian expression of BRAC, a BRET-based autofluorescent Ca2+ indicator

Depositor

Takeharu Nagai

Article

Saito et al PLoS One. 2010 Apr 1;5(4):e9935. doi: 10

Insert

BRAC

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only
AAV-hSyn-jGCaMP7b-XC

Plasmid

#182221

Purpose

Improved genetic encoded calcium sensor based on GCaMP7b.

Depositor

Xiaodong Liu

Article

Geng et al eLife. 2022 Oct 5;11:e76691. doi: 10.755

Insert

jGCaMP7b-XC

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::PuroR-bGHpA

Plasmid

#62439

Purpose

MXS_chaining vector with CMV::PuroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Puromycin with CMV Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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LAMP-CeNL_pcDNA3

Plasmid

#89514

Purpose

Cyan color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Use

Tags

LAMP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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Halo-OPTN

Plasmid

#187900

Purpose

Mammalian cell expression of Halo-tagged OPTN

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

OPTN (OPTN Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-mWasabi-G

Plasmid

#164161

Purpose

Expresses mWasabi under the control of CAG promoter, basic vector, multiclonig site at C terminal

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mWasabi

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR1A-3XFlag-WT-MEFV

Plasmid

#167018

Purpose

Gateway entry plasmid with 3xflag-pyrin (Wild-type)

Depositor

Thomas Henry

Article

Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

Insert

MEFV (MEFV Human)

Use

Gateway entry vector

Tags

3xFlag

Expression

Mutation

Promoter

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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GreenT-EC - GPI

Plasmid

#193946

Purpose

Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC

Use

Tags

GPI-anchor and Igk_secretion - HA-Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLifeAct-miRFP713

Plasmid

#136562

Purpose

F-actin labeling with a near-infrared fluorescent protein miRFP713

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

LifeAct-miRFP713

Use

Tags

miRFP713

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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1/8-NORAD-(A)4x

Plasmid

#199209

Purpose

1/8 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)

Depositor

Esther Braselmann

Article

Sarfraz et al Nat Commun. 2023 Feb 16;14(1):867. doi:

Insert

1/8-NORAD

Use

Tags

Riboglow RNA tag

Expression

Mammalian

Mutation

4 copies of Riboglow tag after stop codon

Promoter

CMV

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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nls-Flamindo2

Plasmid

#73939

Purpose

Expresses biosensor for cAMP in mammalian cells with nuclear localization signal

Depositor

Tetsuya Kitaguchi

Article

Odaka et al PLoS One. 2014 Jun 24;9(6):e100252. doi:

Insert

nls-Flamindo2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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