We narrowed to 14,145 results for: TIM
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Plasmid#20943Purpose3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsmCherryExpressionMammalianMutationH134RPromoterCaMKIIaAvailable SinceJuly 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
RT3GEPIR
Plasmid#111169PurposeTet-regulated miR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseRetroviralMutationWTPromoterT3GAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
Myristoylated optoDroplet
Plasmid#111507PurposeFor membrane localized optoDropletsDepositorInsertCry2 (CRY2 Mustard Weed)
UseLentiviralTagsFUS, Fusion Red, and Myristoylation Sequence (MYR…PromoterSFFVAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
Intron-Tagging-EGFP-Donor
Plasmid#159740PurposeContains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.DepositorInsertEGFP
UseIntron tagging donorAvailable SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOS015E: Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)
Plasmid#163076PurposeEntry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)DepositorInsertmito-roGFP2-Orp1
UseGateway entry vectorAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRS-LacI-GFP
Plasmid#220198Purposemini-Tn7 GFP expression vector - LacI/pLac promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterLacI/pLacAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-YeNL
Plasmid#208849PurposeExpresses FLAG epitope-tagged YeNL "Yellow" BRET reporter in mammalian cells.DepositorInsertYeNL Nanolantern
UseLentiviralTags1xFLAGExpressionMammalianPromoterCMVAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-OeNL
Plasmid#208850PurposeExpresses FLAG epitope-tagged OeNL "Orange" BRET reporter in mammalian cells.DepositorInsertOeNL Nanolantern
UseLentiviralTags1xFLAGExpressionMammalianPromoterCMVAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-BG42-GFP
Plasmid#220202Purposemini-Tn7 GFP expression vector - pBG42 promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG42Available SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMCSF-R-luc
Plasmid#12420DepositorInsertM-CSF receptor promoter fragment (CSF1R Human)
UseLuciferaseAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJH4783
Plasmid#200785PurposePnpr-4 snb-1::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of snb pHluorinDepositorAvailable SinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
Stx4-pmCitrine
Plasmid#92422PurposeExpression of syntaxin-4 C-terminally conjugated to mCitrine.DepositorAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP1
Plasmid#71465PurposeEncodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 1 (SENP1 Human)
TagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-CeNL
Plasmid#208839PurposeExpresses FLAG epitope-tagged CeNL "Blue" BRET reporter in mammalian cells.DepositorInsertCeNL Nanolantern
UseLentiviralTags1xFLAGExpressionMammalianPromoterCMVAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTP1B human
Plasmid#102719Purposehuman PTP1B catalytic domain (residues 1-301) in RP1B vectorDepositorInsertProtein Tyrosine Phosphatase 1B (PTP1B) (PTPN1 Human)
Tags6xHisExpressionBacterialPromoterT7Available SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1deltaH4-H6
Plasmid#71717PurposeMembrane scaffold protein (MSP), helix 4 to 6 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
ExpressionBacterialAvailable SinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSPAsp-hp
Plasmid#48122PurposeShuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoterDepositorInsertpredicted optimal signal peptide for Bacillus megaterium protein secretion
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pN256
Plasmid#188472PurposescDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFPDepositorInserteGFP-WPRE-DAISY-4T
Available SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_H2B_HaloTag9_T2A_EGFP
Plasmid#169332PurposeNuclear expression of HaloTag9 in mammalian cellsDepositorInsertH2B-HaloTag9 (H2BC21 Human)
TagsHaloTagExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIVT/Smarcc1
Plasmid#122145PurposeFor making Smarcc1 RNADepositorAvailable SinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mBlueberry2-P2A-EGFP
Plasmid#184937PurposeExpresses blue fluorescent protein mBlueberry2 and EGFP in mammalian cellsDepositorInsertmBlueberry2-P2A-EGFP
UseAAVExpressionMammalianPromoterCAGAvailable SinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSMOX-mNG::LacI-V3
Plasmid#210224PurposeIntegrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.DepositorInsertmNeongreen Lac repressor fusion protein
UseTrypanosoma brucei expressionPromoterPol II readthrough transcription after integrationAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pssAAV-2-hGFAP-GeNL-WPRE-bGHp(A)
Plasmid#221455PurposeAAV vector expressing GFAP-GeNLDepositorInsertGreen enhanced Nano-lantern
UseAAVPromoterhGFAPAvailable SinceAug. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZR043_Lenti-U6-sgEGFR-MS2-PP7-hPGK-PCP-p65-HSF1-Puro
Plasmid#180267PurposeLentiviral expression vector for CRISPRa-SAM with EGFR targeting sgRNADepositorInsertEGFR sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-CLIP-PD-L2
Plasmid#223587PurposeLentiviral expression of human PD-L2 with CLIP tag in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mTagBFP2-P2A-EGFP
Plasmid#184943PurposeExpresses blue fluorescent protein mTagBFP2 and EGFP in mammalian cellsDepositorInsertmTagBFP2-P2A-EGFP
UseAAVExpressionMammalianPromoterCAGAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP2
Plasmid#71466PurposeEncodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 2 (SENP2 Human)
TagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGF-mSox2-ONL
Plasmid#65719PurposemSox2 reporter plasmid (expresses ONL)DepositorInsertmurine Sox2 promoter and ONL
UseLentiviral and LuciferaseAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cas9 MDC123
Plasmid#59184Purpose2x35S promoting Glycing Max codon optimized Cas9 with bar plant selectionDepositorInsertGlycine Max codon optimized Cas9
UseCRISPRExpressionPlantPromoter2X35SAvailable SinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRA-33-MC_hammer
Plasmid#138989PurposeIVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38DepositorAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-MXT GIRK2
Plasmid#20675DepositorInsertMus musculus G protien coupled inward rectifier K+ channel 2 (Kcnj6 Mouse)
UseXenopus expressionTags3' UTR Xenopus B- globin and 5' UTR Xen…MutationA369VAvailable SinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEB1-moxCerulean3
Plasmid#103975PurposePlasmid encoding moxCerulean3DepositorInsertmoxCerulean3
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F64L, Y66W, …PromoterproCAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)
Plasmid#185918PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337RPromoterCMV and T7Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM6-vioABECD
Plasmid#66535PurposepETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with consensus promoterDepositorInsertsVioA
VioB
VioE
VioC
VioD
UseSynthetic BiologyExpressionBacterialPromoterConsensus T7Available SinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-oG-WPRE-SV40pA
Plasmid#74292PurposepAAV vector to express oG (optimized Glycoprotein) in a Cre-dependent mannerDepositorInsertoG (optimized Glycoprotein)
UseAAVExpressionMammalianMutationchimeric glycoproteinPromoterCAGAvailable SinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS-H2B-mRFP1
Plasmid#53745PurposeContains a fusion of human histone H2B to monomeric red fluorescent protein 1 in the pCS2+ expression vectorDepositorAvailable SinceJuly 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PCNA-IRES-puro2b
Plasmid#26461DepositorAvailable SinceDec. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
G10 Cas9 MDC123
Plasmid#59187PurposeG10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selectionDepositorInsertGlycine Max codon optimized Cas9
UseCRISPRExpressionPlantPromoterG10Available SinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF1alpha-rtTA-Antares
Plasmid#197587PurposeExpresses TET-On reverse transactivator labeled with AntaresDepositorInsertrtTA-P2A-Antares
TagsAntaresExpressionMammalianPromoterEF-1alphaAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBITE-EGFRvIII-F265-OKT3
Plasmid#190679PurposeEncodes an EGFRvIII-CD3 BITE protein (F265 clone)DepositorInsertAnti-EGFRvIII-scFv-F265-modularized-OKT3-HisTag
UseLentiviralTagsmNeonGreenExpressionMammalianPromoterEFSAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only