We narrowed to 8,907 results for: Pol

1. pcDNA5-Flag-BRD4-7D

   Plasmid

   #90007

   Purpose

   expresses 7D mutant BRD4

   Depositor

   Kornelia Polyak

   Article

   Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed 484S to 484D, 488S to 488D, 492S to 492D,…

   Promoter

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5-Flag-BRD4-7A

   Plasmid

   #90006

   Purpose

   expresses 7A mutant BRD4

   Depositor

   Kornelia Polyak

   Article

   Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed 484S to 484A, 488S to 488A, 492S to 492A,…

   Promoter

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II

   Plasmid

   #52093

   Purpose

   Retroviral expression of all four WT CD3 subunits, co-expressed with mAmetrine

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

   Inserts

   CD3 delta (Cd3d Mouse)
   CDC gamma (Cd3g Mouse)
   CD3 epsilon (Cd3e Mouse)
   CD3 zeta (Cd247 Mouse)
   

   Use

   Retroviral

   Tags

   F2A, IRES-EGFP, P2A, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II

   Plasmid

   #52092

   Purpose

   Retroviral expression of all four WT CD3 subunits, co-expressed with EGFP

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

   Inserts

   CD3 delta (Cd3d Mouse)
   CDC gamma (Cd3g Mouse)
   CD3 epsilon (Cd3e Mouse)
   CD3 zeta (Cd247 Mouse)
   

   Use

   Retroviral

   Tags

   F2A, IRES-EGFP, P2A, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGLU_82

   Plasmid

   #225783

   Purpose

   RS421 library variant 13550

   Depositor

   Nili Ostrov

   Insert

   13550_[abr]
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGLU_100

   Plasmid

   #225813

   Purpose

   RS421 library variant 13550

   Depositor

   Nili Ostrov

   Insert

   13550_[Himar]
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGLU_118

   Plasmid

   #225798

   Purpose

   RS421 library variant 13550

   Depositor

   Nili Ostrov

   Insert

   13550_[Tn5]
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSLfa-PUb-MCS

   Plasmid

   #52908

   Purpose

   Aedes aegypti polyubiquitin promoter followed by MCS

   Depositor

   Zach Adelman

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Ae aegptyi polyubiquitin

   Available since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOpen-T4gene43

   Plasmid

   #165524

   Purpose

   Polymerase that replicates viral genomic DNA.

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   T4 DNA Polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pOpen_taq

    Plasmid

    #153315

    Purpose

    Bacterial expression of Taq polymerase

    Depositor

    Drew Endy

    Insert

    Taq polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pOpen-T7gene1

    Plasmid

    #165505

    Purpose

    Highly processive DNA-dependent RNA polymerase derived from T7 bacteriophage

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    T7 RNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pBS-3xpA

    Plasmid

    #176505

    Purpose

    3x SV40 polyA sequences

    Depositor

    Mario Capecchi

    Insert

    3x SV40 polyA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBS-2xpA

    Plasmid

    #176504

    Purpose

    2x SV40 polyA sequences

    Depositor

    Mario Capecchi

    Insert

    2x SV40 polyA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBS-4xpA

    Plasmid

    #176506

    Purpose

    4x SV40 polyA sequences

    Depositor

    Mario Capecchi

    Insert

    4x SV40 polyA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. L4437

    Plasmid

    #1652

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    RNAi

    Tags

    pes10UAS-270, pes10Tsa, IVSA, SV40NLS, T7polN, T7…

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-VSVL

    Plasmid

    #64085

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain polymerase gene

    Depositor

    Ian Wickersham

    Insert

    VSV polymerase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJY4092

    Plasmid

    #113334

    Purpose

    Vector for Hermes transposition; Hermes TIR-flanked AvrII cut site; recoded transposase driven by TEF1 promoter

    Depositor

    Suzanne Sandmeyer

    Insert

    codon-optimized Hermes transposase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. C95m

    Plasmid

    #121001

    Purpose

    MoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    T7 RNA polymerase (Andy please check!)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 371-A

    Plasmid

    #97026

    Purpose

    yORF

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOpen-T5gene122

    Plasmid

    #165544

    Purpose

    Possesses two enzymatic activities: DNA synthesis (polymerase) and exonucleolytic activity that degrades ssDNA in the 3'-5' direction for proofreading purposes.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    T5 DNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. 371-U

    Plasmid

    #97036

    Purpose

    MYFQSNA-yORF

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    MYFQSNA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAG-B19L

    Plasmid

    #59922

    Purpose

    Expression vector for Rabies virus SAD B19 RNA dependent RNA polymerase gene

    Depositor

    Ian Wickersham

    Insert

    Rabies virus RNA dependent RNA polymerase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEIAV-SIN6.1 CBGFPW

    Plasmid

    #44173

    Depositor

    John Olsen

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    chromophore mutations

    Promoter

    CB, CMV enhancer/chicken beta-actin promoter

    Available since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pIU211

    Plasmid

    #44036

    Depositor

    Dane Wittrup

    Article

    Boder et al Nat Biotechnol. 1997 Jun;15(6):553-7.

    Insert

    AGA1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pOpen-T3gene1

    Plasmid

    #165576

    Purpose

    DNA-dependent RNA polymerase derived from T3 bacteriophage that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    T3 RNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pOpen-dbh

    Plasmid

    #165513

    Purpose

    Thermostable gamma-family lesion-bypass DNA Polymerase that efficiently synthesizes DNA across a variety of DNA template lesions.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    Sulfolobus DNA Polymerase IV
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. CRY2olig-PR

    Plasmid

    #218917

    Purpose

    Expression of CRY2olig-mCherry fused to a codon-optimised poly-PR sequence

    Depositor

    Tatyana Shelkovnikova

    Insert

    CRY2olig-mCherry tagged poly-PR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CMV

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CRY2olig-GP

    Plasmid

    #218918

    Purpose

    Expression of CRY2olig-mCherry fused to a codon-optimised poly-GP sequence

    Depositor

    Tatyana Shelkovnikova

    Insert

    CRY2olig-mCherry tagged poly-GP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CMV

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CRY2olig-GR

    Plasmid

    #218919

    Purpose

    Expression of CRY2olig-mCherry fused to a codon-optimised poly-GR sequence

    Depositor

    Tatyana Shelkovnikova

    Insert

    CRY2olig-mCherry tagged poly-GR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CMV

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pIC195

    Plasmid

    #69730

    Purpose

    Retroviral expression of Polo-Like Kinase 1 (Plk1)

    Depositor

    Iain Cheeseman

    Insert

    Polo-Like Kinase 1 (Plk1) (PLK1 Human)
    

    Use

    Retroviral

    Tags

    eYFP-TEV-S

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge