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We narrowed to 34,434 results for: CaS

Showing: 4351 - 4400 of 34434 results

  1. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-6

    Plasmid
    #129034
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA6 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA6 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129034-plasmid-map-sequence-id-256162

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  2. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-7

    Plasmid
    #129035
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA7 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA7 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129035-plasmid-map-sequence-id-256164

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  3. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-9

    Plasmid
    #129037
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA9 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA9 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129037-plasmid-map-sequence-id-256167

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  4. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-10

    Plasmid
    #129038
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA10 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA10 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129038-plasmid-map-sequence-id-256168

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  5. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-11

    Plasmid
    #129039
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA11 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA11 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129039-plasmid-map-sequence-id-256194

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  6. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-2

    Plasmid
    #129030
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA2 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA2 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129030-plasmid-map-sequence-id-256140

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  7. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-3

    Plasmid
    #129031
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA3 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA3 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129031-plasmid-map-sequence-id-256155

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  8. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-4

    Plasmid
    #129032
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA4 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA4 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129032-plasmid-map-sequence-id-256156

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  9. pDGB3 omega1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1657)

    Plasmid
    #160640
    Purpose
    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).
    Depositor
    Diego Orzaez
    Insert
    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    Use
    CRISPR
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, 35S
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    160640-plasmid-map-sequence-id-321670

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  16. IF405: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-p300core

    Plasmid
    #121825
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    Sa-dCas9/p300core (open)
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121825-plasmid-map-sequence-id-237377

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  17. IP803: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-Dnmt3a3L

    Plasmid
    #121826
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    Sa-dCas9/Dnmt3a-3L (open)
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121826-plasmid-map-sequence-id-237378

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  18. LF901: pMAGIC (L1-R5) mU6::SaCas9 gRNA scaffold

    Plasmid
    #121811
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121811-plasmid-map-sequence-id-237356

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  19. IA304: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-VPR

    Plasmid
    #121822
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    Sa-dCas9/VPR (open)
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121822-plasmid-map-sequence-id-237380

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  20. IB801: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-KRAB

    Plasmid
    #121823
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    Sa-dCas9/KRAB (open)
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121823-plasmid-map-sequence-id-237375

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  26. BPK4387 - human expression plasmid for SpCas9 Cluster 5

    Plasmid
    #101191
    Purpose
    Human expression plasmid for SpCas9 Cluster 5 variant: CMV-T7-hSpCas9-Cluster5(K918A, V922A, R925A)-NLS(SV40)-3xFLAG
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster5(K918A/V922A/R925A)
    Use
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    K918A/V922A/R925A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101191-plasmid-map-sequence-id-194254

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  27. MMW3645 - human expression plasmid for SpCas9 Cluster 3

    Plasmid
    #101187
    Purpose
    Human expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAG
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster3(T657A/G658A/W659A/R661A)
    Use
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    T657A/G658A/W659A/R661A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101187-plasmid-map-sequence-id-194250

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  28. MMW3770 - human expression plasmid for SpCas9 Cluster 4

    Plasmid
    #101189
    Purpose
    Human expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAG
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster4(F491A/M495A/T496A/N497A)
    Use
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    F491A/M495A/T496A/N497A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101189-plasmid-map-sequence-id-194252

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  29. MMW2993 - human expression plasmid for SpCas9 Cluster 2

    Plasmid
    #101185
    Purpose
    Human expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAG
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster2(G582A/V583A/E584A/D585A/N588A)
    Use
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    G582A/V583A/E584A/D585A/N588A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101185-plasmid-map-sequence-id-194249

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  33. pCASCADE-fabI-gltA1-gltA2-udhA-zwf

    Plasmid
    #87148
    Purpose
    fabI-gltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    fabI-gltA1-gltA2-udhA-zwf guide array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87148-plasmid-map-sequence-id-175306

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  34. pCASCADE-fabI-gltA1-gltA2-udhA-gapAP1

    Plasmid
    #87154
    Purpose
    fabI-gltA1-gltA2-udhA-gapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    fabI-gltA1-gltA2-udhA-GapAP1 guide array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87154-plasmid-map-sequence-id-174942

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  38. tFucci(CA)5

    Plasmid
    #153521
    Purpose
    New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.
    Depositor
    Atsushi Miyawaki
    Article
    Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.
    Insert
    tFucci(CA)5.
    Use
    Tags
    AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    153521-plasmid-map-sequence-id-308996

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  43. pCuo CA Rac1

    Plasmid
    #84643
    Purpose
    constitutively active Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.
    Depositor
    Sanjay Kumar
    Article
    MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94
    Insert
    Rac1 (RAC1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Constitutively Active, Q61L
    Promoter
    cumate-inducible promoter
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    84643-plasmid-map-sequence-id-175059

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  47. pSLIK CA Rac1

    Plasmid
    #84605
    Purpose
    Lentiviral expression of doxycycline-inducible constitutively active Rac1 and co-expression of Venus
    Depositor
    Sanjay Kumar
    Article
    MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94
    Insert
    Rac1 (RAC1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Constitutively Active, Q61L
    Promoter
    Available since
    Nov. 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  49. pENTR11 CA NFAT1

    Plasmid
    #11792
    Depositor
    Anjana Rao
    Insert
    NFAT1 (Nfatc2 Mouse)
    Use
    Tags
    HA
    Expression
    Bacterial
    Mutation
    Constitutively active: multiple serine to alanine…
    Promoter
    Available since
    Aug. 7, 2006
    Availability
    Academic Institutions and Nonprofits only
    11792-plasmid-map-sequence-id-229776

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  50. PB-CA sfGFP

    Plasmid
    #159094
    Purpose
    PB-CA sfGFP piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    sfGFP
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159094-plasmid-map-sequence-id-316224

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Showing: 4351 - 4400 of 34434 results