8,727 results

1. pJRH-1362 scFv entry plasmid

   Plasmid

   #201912

   Purpose

   Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUW-tetO-hOKMS

   Plasmid

   #51543

   Purpose

   Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2

   Depositor

   Tarjei Mikkelsen

   Article

   Cacchiarelli et al Cell. 2015 Jul 16;162(2):412-24. doi: 10

   Insert

   hOKMS (MYC, KLF4, SOX2, POU5F1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-mCherry-Mango II x 24

   Plasmid

   #127587

   Purpose

   Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter

   Depositor

   David Rueda

   Article

   Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi:

   Insert

   mCherry-Mango II x24
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE Tet ON

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABE HAII

   Plasmid

   #14738

   Depositor

   Adrienne Cox

   Article

   Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pdCas9-bacteria

   Plasmid

   #44249

   Purpose

   aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dCas9 (bacteria)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   pLtetO-1

   Available since

   April 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC1-SypHer3s-dmito

   Plasmid

   #108119

   Purpose

   Mitochondrial ratiometric pH probe with enhanced brightness for mammalian cells expression. Probe is localized in mitochondrial matrix.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

   Insert

   SypHer3s-dmito
   

   Use

   Tags

   ratiometric YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-EGFP-HaloTag2

   Plasmid

   #41742

   Depositor

   Craig Crews

   Article

   Neklesa et al Nat Chem Biol. 2011 Jul 3;7(8):538-43. d

   Insert

   Halotag 2
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPR

   Plasmid

   #42875

   Purpose

   A crRNA expression plasmid for targeting a specific sequence.

   Depositor

   Luciano Marraffini

   Article

   Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   CRISPR-BsaI
   

   Use

   CRISPR; E.coli

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-ERBB2

   Plasmid

   #116734

   Purpose

   Lentiviral expression of ERBB2

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. 7TFC

    Plasmid

    #24307

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    7xTcf-FFluc // SV40-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLIK-Neo

    Plasmid

    #25735

    Purpose

    3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Neomycin resistance gene coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL2-eNOSPromoter-Luciferase

    Plasmid

    #19297

    Depositor

    Charles Lowenstein

    Article

    Arnet et al J Biol Chem. 1996 Jun 21. 271(25):15069-

    Insert

    endothelial nitric oxide synthase promoter (NOS3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pSLCAR-CD19-CD3z

    Plasmid

    #135993

    Purpose

    Modular CD3z alone CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Scott McComb

    Article

    Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

    Insert

    FMC63-3z-P2A-EGFP (CD247, CD28 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-GFP-Gal8

    Plasmid

    #127191

    Purpose

    Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.

    Depositor

    Jordan Green

    Article

    Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

    Insert

    GFP-Gal8 (LGALS8 Human)
    

    Use

    Tags

    Gal8 is fused to the c-terminus of GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. JDS246

    Plasmid

    #43861

    Purpose

    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters

    Depositor

    Keith Joung

    Insert

    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    

    Use

    CRISPR

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSIH1-puro-STAT3 shRNA

    Plasmid

    #26596

    Depositor

    Frank Sinicrope

    Article

    Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

    Insert

    STAT3 shRNA (STAT3 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. mito-dendra2

    Plasmid

    #55796

    Purpose

    photo-activatable mitochondrial marker for expression in mammalian cells

    Depositor

    David Chan

    Article

    Pham et al Genesis. 2012 Nov;50(11):833-43. doi: 10

    Insert

    mito-Dendra2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3993 pET ubcH7

    Plasmid

    #8654

    Depositor

    Peter Howley

    Article

    Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

    Insert

    UbcH7 (UBE2L3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pLenti-HRE-dUnaG

    Plasmid

    #124372

    Purpose

    UnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signaling

    Depositor

    Roland Friedel

    Article

    Sattiraju et al Immunity. 2023 Jul 7:S1074-7613(23)00274

    Insert

    HRE-dUnaG
    

    Use

    Lentiviral

    Tags

    PEST-degron and Myc tag

    Expression

    Mutation

    Promoter

    HRE (HIF responsive element 5x + CMV minimal prom…

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLNCX2-mCherry-CHMP4B

    Plasmid

    #116923

    Purpose

    expresses mCherry-CHMP4B in mammalian cells

    Depositor

    Sanford Simon

    Article

    Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

    Insert

    CHMP4B (CHMP4B Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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