We narrowed to 25,909 results for: Nes
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Plasmid#184920PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX304-CAR-V5
Plasmid#82723PurposeExpresses V5 tagged coxsackievirus and adenovirus receptor (CAR).DepositorInsertcoxsackievirus and adenovirus receptor (CXADR Human)
UseLentiviralTagsV5ExpressionMammalianAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMax_GFP_C289T
Plasmid#177826PurposeSite-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).DepositorInsertGFP_C289T
ExpressionMammalianMutationC289TPromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-p38alpha
Plasmid#47575PurposeBacterial expression plasmid for GST-3xFlag-tagged p38alphaDepositorAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)
Plasmid#218513Purposereplicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferaseDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of the Plac_mrfp cassettePromoterPgyrA_ermBL-ermB'Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-DEST-MatryoshCaMP6s
Plasmid#100025PurposeMammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
TagsV5 Epitope TagExpressionMammalianPromoterCMVAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:GrRenilla
Plasmid#118067PurposeTranscriptional unit for constitutive expression of GrRenilla in mammalian cellsDepositorInsertCMV:GreenRenilla:bGHF
UseLuciferase and Synthetic Biology; Assembly of gb2…ExpressionMammalianPromoterCMVAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
tet_pLKO.1_puro_shLuc
Plasmid#136587PurposeExpresses an inducible short hairpin targeting firefly luciferase sequenceDepositorInsertshLuc
UseLentiviralExpressionMammalianAvailable SinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYES260-sc.eEF2
Plasmid#85119Purposeyeast expression of EEF2DepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:ELuc
Plasmid#118062PurposeTranscriptional unit for constitutive expression of ELuc in mammalian cellsDepositorInsertCMV:ELuc:bGH
UseLuciferase and Synthetic Biology; Assembly of gb2…ExpressionMammalianAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMax_BFP_A191G
Plasmid#177823PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).DepositorInserttagBFP_A191G
ExpressionMammalianMutationA191GPromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
G-GECO1-Orai3
Plasmid#73563PurposeGreen fluorescent reporter of Orai3-associated calcium influxDepositorInsertG-GECO1-Orai3 (ORAI3 Synthetic, Human)
TagsG-GECO1ExpressionMammalianPromoterCMV Immdeiate EarlyAvailable SinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDule-Acd82
Plasmid#174099PurposeAcridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.DepositorInsertAcridone 82 tRNA synthetase and tRNA
ExpressionBacterialMutationN311S, C313G, V366A, W382TAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5xNF-kb_RE
Plasmid#118050PurposeGBPart - Operator/DNA Response element - 5 copies of the NF-kb DNA binding motifDepositorInsert5 copies of the NF-kb DNA binding motif
UseSynthetic Biology; Domestication of dna parts for…Available SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_mic
Plasmid#184916PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX302-DUSP19-V5 puro
Plasmid#87772PurposeExpresses V5 tagged human DUSP19DepositorAvailable SinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pB-Ric8b-Integration
Plasmid#129457PurposepiggyBac transposon vector that Inducibly (Tet-On) expresses Ric8bDepositorAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-CSE1L zeo
Plasmid#192320PurposeLentiviral expression vector for an inducible 3xFLAG-CSE1LDepositorAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-CSE1L
Plasmid#192295PurposeGateway entry vector for an inducible 3xFLAG-CSE1LDepositorAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBK94 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (KanR)
Plasmid#179078PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK03 pCAS-Tyr-[gRNA: BsaI GFP dropout] (HygR,AmpR)
Plasmid#178987PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKMD111e - intein-Cas9(S219-G521R)
Plasmid#64192PurposeExpresses intein-Cas9(S219-G521R) in mammalian cellsDepositorInsertmammalian codon-optimized S. pyogenes intein-Cas9(S219-G521R) - NLS - 3x FLAG
UseCRISPRTagsNLS - 3x FLAG (C terminal on insert)ExpressionMammalianPromoterCMVAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBBK04 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitHygR,AmpR)
Plasmid#178988PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-KPNB1
Plasmid#192301PurposeGateway entry vector for an inducible 3xFLAG-KPNB1DepositorAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBK07 pCAS-Tyr-[gRNA: BsaI GFP dropout] (LEU2,AmpR)
Plasmid#178991PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = LEU2DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
YE1-BE4max-CP1028
Plasmid#138160PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE1)-nSpCas9 (CP1028) -UGI-UGI
TagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E; within Cas9 (CP1028…PromoterCMVAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB2909(XII-5 MarkerFree)
Plasmid#73281PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-ERK2
Plasmid#47573PurposeBacterial expression plasmid for GST-3xFlag-tagged ERK2DepositorAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
KIF5C(1-560)-2xmCherry-EF(C)
Plasmid#61664PurposeTandem mCherry-labeled, truncated kinesin-1 fused to EF Hand linkerDepositorInsertKIF5C(1-560)-2xmCherry-EF(C) (Kif5c Rat)
ExpressionMammalianAvailable SinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-KPNA1 zeo
Plasmid#192329PurposeLentiviral expression vector for an inducible 3xFLAG-KPNA1DepositorAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAEL006511-Cas9
Plasmid#100580PurposeThe promoter of AAEL006511 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL006511-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL006511 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-T7-hOsa1
Plasmid#17986DepositorAvailable SinceAug. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
MSCV P2Gm p53
Plasmid#19751DepositorAvailable SinceDec. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET15b-sfGO-Matryoshka-LE-LP
Plasmid#100018PurposeBacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkersDepositorInsertcpsfGFP-LSSmOrange
Tags6x HIS tagExpressionBacterialMutationLeu and Glu N-term linker and Leu and Pro C-term …PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
UAS-u(S)Cas9
Plasmid#127383PurposeExpresses Cas9 at high levelDepositorInsertuORF(S)-Cas9
UseCRISPRExpressionInsectPromoterUASTAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
PatoL-OG241
Plasmid#72841PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.DepositorInsertPatoL
ExpressionBacterialPromoterPatoLAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
Orange Nano-lantern(Ca2+)/pcDNA3
Plasmid#71346PurposeExpress Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cellDepositorInsertOrange Nano-lantern(Ca2+)
ExpressionMammalianAvailable SinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBBK95 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (HygR)
Plasmid#179079PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIMVAd40R
Plasmid#98309PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of GAL promoters; integrated into ERG9 locus;DepositorInsertERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1ExpressionYeastAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRE-iRFP670
Plasmid#82696PurposeExpresses CRE and iRFP670 under two separate PGK promoters.DepositorInsertCRE, iRFP670
UseCre/LoxAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV EFIa DIO LMO11 (GeNL_SS-ChRmine)
Plasmid#219426PurposeCre-dependent expression of luciferase opsin fusion protein (luminopsin) for bioluminescent optogeneticsDepositorInsertmNeonGreen, ISN-IE, ChRmine
UseAAVTagsGaussia luciferase signal sequencePromoterEF-1alphaAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB-RTP2-Integration
Plasmid#129459PurposepiggyBac transposon vector that Inducibly (Tet-On) expresses RTP2DepositorAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin
Plasmid#199585PurposeExpress tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vectorDepositorInsertsERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
AkaLuc
Blasticidin
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM6-RgTALsyn-HpaBC
Plasmid#100949PurposeExpression of RgTALsyn, HpaB, and HpaC from inducible T7 promoterDepositorInsertsRgTALsyn
HpaB
HpaC
UseSynthetic BiologyExpressionBacterialMutationSilent mutation to remove internal NdeI site on T…PromoterT7Available SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV FLIPi P2G_Thy1.1 PTEN
Plasmid#19749DepositorAvailable SinceDec. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-BCAS2 zeo
Plasmid#192319PurposeLentiviral expression vector for an inducible 3xFLAG-BCAS2DepositorAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET a MatryoshCaMP6s
Plasmid#100019PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
Tags6x HIS tag and Xpress_EK tagExpressionBacterialPromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pgRGFP
Plasmid#82695PurposeExpresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterPGK, U6Available SinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX302 IL10RA-V5 puro
Plasmid#47552PurposeLentiviral expression of V5-tagged IL10RA in mammalian cellsDepositorAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b-eGO-Matryoshka-LE-LP
Plasmid#100017PurposeBacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkersDepositorInsertcpEGFP-LSSmOrange
Tags6x HIS tagExpressionBacterialMutationLeu and Glu N-term linker and Leu and Pro C-term …PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only