We narrowed to 59,899 results for: %s

1. Epac-S-H151

   Plasmid

   #170346

   Purpose

   6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Insert

   6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   SP6

   Available Since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJ414 - NERD-S

   Plasmid

   #136388

   Purpose

   Expresses NERD-S static protein

   Depositor

   Roberto Chica

   Article

   Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

   Insert

   NERD-S
   

   Tags

   6x-His tag (N-term of insert)

   Expression

   Bacterial

   Mutation

   Mutation from GB1 WT: Y3F-L7I-A34F-V39I-V54I

   Promoter

   T7 promoter

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-S*HiGlpG

   Plasmid

   #158534

   Purpose

   Bacterial expression of rhomboid protease mutant S*HiGlpG

   Depositor

   Sinisa Urban

   Article

   Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

   Insert

   S*HiGlpG
   

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Sector 1 and 2 mutations

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. U6-Mos-s

   Plasmid

   #120533

   Purpose

   ssRNA expression for dsRNA production

   Depositor

   Petr Svoboda

   Insert

   Mos (Mos Mouse)
   

   Use

   RNAi

   Mutation

   A>G (see depositor comments)

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-Mos-s

   Plasmid

   #120525

   Purpose

   ssRNA expression for dsRNA production

   Depositor

   Petr Svoboda

   Insert

   Mos (Mos Mouse)
   

   Use

   RNAi

   Mutation

   A>G (see comments)

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. U6-Lin28a-s

   Plasmid

   #120535

   Purpose

   ssRNA expression for dsRNA production

   Depositor

   Petr Svoboda

   Insert

   Lin28 (Lin28a Mouse)
   

   Use

   RNAi

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-Lin28a-s

   Plasmid

   #120527

   Purpose

   ssRNA expression for dsRNA production

   Depositor

   Petr Svoboda

   Insert

   Lin28 (Lin28a Mouse)
   

   Use

   RNAi

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. S. aureus DHPS

   Plasmid

   #64079

   Purpose

   Expression of Staphylococcus aureus dihydropteroate synthase

   Depositor

   Vern Schramm

   Article

   Stratton et al ACS Chem Biol. 2015 Oct 16;10(10):2182-6

   Insert

   dihydropteroate synthase
   

   Tags

   Histidine tag

   Expression

   Bacterial

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

9. YA1627: pAAV_hSyn-Dio-paQuasAr3-s-P2A-CheRiff-s

   Plasmid

   #107704

   Purpose

   in vivo voltage imaging

   Depositor

   Adam Cohen

   Article

   Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

   Insert

   paQuasAr3-P2A-CheRiff
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3 Ras-LOCKR-S

    Plasmid

    #216496

    Purpose

    FRET-based reporter for Ras activity, untargeted

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    Ras-LOCKR-S
    

    Expression

    Mammalian

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pL0M-S-mKOκ-EC18154

    Plasmid

    #137077

    Purpose

    Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

    Depositor

    Julian Hibberd

    Article

    Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

    Insert

    mKOκ DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pL0M-S-mTurquoise2-EC18152

    Plasmid

    #137074

    Purpose

    Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

    Depositor

    Julian Hibberd

    Article

    Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

    Insert

    mTurquoise2 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pαH-S-GSAS/D614G

    Plasmid

    #164566

    Purpose

    SARS-CoV-2 Spike protein with D614G mutation (S-GSAS-D614G variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-GSAS-D614G variant)
    

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACE-GIRK4-mC-S

    Plasmid

    #172427

    Purpose

    Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

    Insert

    GIRK4 (KCNJ5 Human)
    

    Tags

    mCherry Streptag II

    Expression

    Insect

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIRESh-GFPII-S-Dlk1

    Plasmid

    #108931

    Purpose

    GFP-tagged vector expressing secreted isoform of Dlk1

    Depositor

    Anne Ferguson-Smith

    Article

    Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

    Insert

    Dlk1 (Dlk1 Mouse)
    

    Tags

    GFPII

    Expression

    Mammalian

    Mutation

    contains sequences from full length Dlk1A up to t…

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pL0M-S-mNeonGreen-EC18153

    Plasmid

    #137075

    Purpose

    Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

    Depositor

    Julian Hibberd

    Article

    Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

    Insert

    mNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Sema6b(S)-AP-His

    Plasmid

    #72040

    Purpose

    Expresses the extracellular region of the Sema6B protein (truncated at extracellular domain cleavage site; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6b (Sema6b Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pαH-S-GSAS-OMICRON

    Plasmid

    #180423

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

    Insert

    Spike (S-GSAS-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pαH-S-RRAR/D614G

    Plasmid

    #164570

    Purpose

    SARS-CoV-2 Spike protein with D614G mutation and Furin site restored (S-RRAR-D614G variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-RRAR-D614G variant)
    

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    D614G

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pαH-S-RRAR-OMICRON

    Plasmid

    #180424

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side intact

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

    Insert

    Spike (S-RRAR-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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