9,002 results

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pCMV4-3 HA/IkB-alpha

Plasmid

#21985

Depositor

Warner Greene

Article

Sun et al Mol Cell Biol. 1996 Mar . 16(3):1058-65.

Insert

I-kappa B alpha (NFKBIA Human)

Tags

3x HA

Expression

Mammalian

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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p45sheLL

Plasmid

#37323

Depositor

John Schiller

Article

Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

Insert

HPV45L1+L2

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pSpCas9 (PX165)

Plasmid

#48137

Purpose

Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-mGFP-Actin

Plasmid

#21948

Depositor

Ryohei Yasuda

Article

Murakoshi et al Brain Cell Biol. 2008 Aug . 36(1-4):31-4

Insert

betaActin (ACTB Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau

Plasmid

#46904

Purpose

expresses EGFP tagged Tau in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

MAPT (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

This WT htau construct contains human four-repeat…

Promoter

CMV

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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SaABE8e

Plasmid

#138500

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSaCas9

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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CNR1-Tango

Plasmid

#66254

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CNR1 (CNR1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pCXN2-HA-AT1R-YFP

Plasmid

#101659

Purpose

Expresses AT1R with HA tag and YFP in mammalian cells.

Depositor

Yusuke Ohba

Article

Inuzuka et al Sci Rep. 2016 Feb 9;6:21613. doi: 10.103

Insert

Angiotensin II type 1 receptor (AGTR1 Human)

Tags

HA and YFP (Venus)

Expression

Mammalian

Promoter

CAG promoter

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBMN FKBP(DD)-YFP

Plasmid

#31763

Depositor

Thomas Wandless

Article

Banaszynski et al Cell. 2006 Sep 8;126(5):995-1004.

Insert

FKBP(DD)-YFP-HA (FKBP1A Human)

Use

Retroviral

Tags

HA and YFP

Mutation

F36V, L106P on FKBP12

Promoter

LTR

Available Since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2+HyPer7-NES

Plasmid

#136467

Purpose

Mammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Nuclear export signal from the HIV Rev protein

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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GLP1R-Tango

Plasmid

#66295

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GLP1R (GLP1R Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-Eco

Plasmid

#35617

Depositor

Arthur Nienhuis, Patrick Salmon

Insert

Ecotropic MLV envelope

Expression

Mammalian

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only

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mScarlet-I-LaminB

Plasmid

#98831

Purpose

In vivo visualization of the nuclear envelope (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

CytERM

Tags

mScarlet-I

Expression

Mammalian

Mutation

*(see below)

Promoter

CMV

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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GPD-roGFP

Plasmid

#49436

Purpose

Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cells

Depositor

Paul Schumacker

Article

Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

Insert

GPD-roGFP2

Use

Adenoviral

Tags

Glycerol phosphate dehydrogenase, 5' to roGF…

Expression

Mammalian

Mutation

C48S, Q80R, S147C, and Q204C in Clontech's G…

Promoter

CMV

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_AGIA-AirID

Plasmid

#182210

Purpose

Express AirID in mammalian cells.

Depositor

Sohei Ito, Shogo Nakano, Akira Nozawa, Tatsuya Sawasaki

Article

Kido et al Elife. 2020 May 11;9. pii: 54983. doi: 1

Insert

AGIA-AirID

Tags

AGIA-tag

Expression

Mammalian

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pPAmCherry-a-tubulin

Plasmid

#31930

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Tags

a-tubulin

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-beta-3

Plasmid

#27289

Depositor

Timothy Springer

Article

Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

Insert

integrin beta 3 (ITGB3 Human)

Tags

His and Myc

Expression

Mammalian

Mutation

n/a

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pTriEx4-Rac1-2G

Plasmid

#66110

Purpose

Second generation Rac1 FRET biosensor

Depositor

Olivier Pertz

Article

Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

Insert

Second generation Rac1 FRET biosensor

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPar2

Plasmid

#53228

Purpose

mammalian expression of human Par2

Depositor

Shaun Coughlin

Article

Bohm et al Biochem J. 1996 Mar 15;314 ( Pt 3)(Pt 3)

Insert

Par2 (F2RL1 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

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anxA2-GFP

Plasmid

#107196

Purpose

Expresses human annexin A2-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

annexin A2 (ANXA2 Human)

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

Ala residue 65 in human sequence replaced by Glu …

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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