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Plasmid#160883PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertrbcS-S2, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S2: T7, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL3664
Plasmid#162240PurposeTranscribes E. coli SRP RNA sequence for SHAPE-SeqDepositorInsertsE. coli SRP RNA
HDV
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
BB1_23_eGFP
Plasmid#98518PurposeBB1 with insert: CDS eGFPDepositorInserteGFP
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-2+t
Plasmid#48148PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
SMT205
Plasmid#134817PurposeSMT201 with mutated RBS for DHFR, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
UseTagsExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pAL950
Plasmid#119748Purposeprecursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to CysteineDepositorInsertprecusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
UseTagsnoneExpressionBacterialMutationInternal NdeI site removed and mutations Alanine …PromoterT7Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pJOE6089.4
Plasmid#135081PurposepBR322-based L-rhamnose-inducible vector.DepositorInsertP-rhaP-BAD-eGFP-Strep-tag II
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pLAT1
Plasmid#98509PurposeBB1 with insert: promoter PLAT1DepositorInsertLAT1 promoter
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pPFK300
Plasmid#98510PurposeBB1 with insert: promoter PPpPfkDepositorInsertPpPfk promoter
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pDAS2
Plasmid#98513PurposeBB1 with insert: promoter PDAS2DepositorInsertDAS2 promoter
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBEST_OR2-OR1-Pr_UTR1-deGFP-T500 BsaI, BbsI-safe (105p)
Plasmid#70194PurposeDerived from 21 with silent mutations to make BsaI, BbsI compatibleDepositorInsertdeGFP
UseTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046831
Plasmid#145671PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;MycExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET3a pro-hRegIIIa
Plasmid#64935Purposebacterial expression of full length human RegIIIalphaDepositorInsertRegIIIa (REG3A Human)
UseTagsExpressionBacterialMutationmutations made in 5' end to relax secondary …PromoterT7Available sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pHL 1405
Plasmid#53010PurposepLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
DsrA sRNA
hfq
sodB
UseSynthetic BiologyTagsgfpExpressionBacterialMutationcontains -56 to +141 relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAGM_Puro_shRNA n.2_mTet3
Plasmid#79625PurposeExpression of shRNA for mouse Tet3DepositorInsertTet3 (Tet3 Mouse)
UseLentiviralTagsnoneExpressionMutationPromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAGM_Puro_shRNA n.1_mTet3
Plasmid#79624PurposeExpression of shRNA for mouse Tet3DepositorInsertTet3 (Tet3 Mouse)
UseLentiviralTagsnoneExpressionMutationPromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT-S15
Plasmid#127822PurposeProduction of recombinant human ribosomal protein S15DepositorInsertRPS15 (RPS15 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pRPL2A
Plasmid#98508PurposeBB1 with insert: promoter PRPL2ADepositorInsertRPL2A promoter
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT-G-S15
Plasmid#127823PurposeProduction of recombinant human ribosomal protein S15DepositorInsertRPS15 (RPS15 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pDAS1
Plasmid#98515PurposeBB1 with insert: promoter PDAS1DepositorInsertDAS1 promoter
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pHL1855
Plasmid#53041PurposePLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st3):csrADepositorInsertsGFP
tetR
csrA
UseSynthetic BiologyTagsLVA, RBS(st3), and glgC leaderExpressionBacterialMutationPromoterpConNoHind and pLlac0-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
EC.SurA.pTYB1.a21-281&390-428.L239R
Plasmid#15959DepositorInsertEscherichia coli surA delta PPIase domain 2
UseTagsinteinExpressionBacterialMutationresidues 282-389 deleted; L239R mutationPromoterAvailable sinceNov. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAX1_HS∆P2
Plasmid#225197PurposeUsed to create a markerless deletion of the E. coli HS P2 phageDepositorInsertP2 phage homology arms
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only