We narrowed to 14,145 results for: TIM
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Plasmid#76714Purpose3rd generation lentiviral gRNA plasmid targeting human PIK3C2BDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only
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pCAG-hCjeCas9-NLS(SV40)-3xFLAG (KAC579)
Plasmid#133789PurposeCAG promoter expression plasmid for human codon optimized CjeCas9 nuclease with C-terminal NLS (SV40)DepositorInserthuman codon optimized CjeCas9
TagsNLS(SV40)-3xFLAGExpressionMammalianMutationn/aPromoterCAGAvailable SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-EGFP_opt
Plasmid#106334PurposeGateway Entry vector for EGFP (codon optimized for efficient gene synthesis)DepositorInsertEGFP
UseGateway entry vectorExpressionBacterial and MammalianMutationcodon optimized for gene synthesis without affect…Available SinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
TK1 gRNA (BRDN0001145069)
Plasmid#76424Purpose3rd generation lentiviral gRNA plasmid targeting human TK1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
NUAK1 gRNA (BRDN0001162598)
Plasmid#75577Purpose3rd generation lentiviral gRNA plasmid targeting human NUAK1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001146004)
Plasmid#80174Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a(ICOS)
Plasmid#177903PurposeBacterial expression of his10-tagged human ICOS Intracellular DomainDepositorInsertICOS (aa 162-199 only) (ICOS Human)
Tags10xHis-Thrombin cut site-T7 tagExpressionBacterialPromoterT7Available SinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shJUND puro
Plasmid#58698PurposeLentiviral shRNA vector for knockdown of human JUNDDepositorAvailable SinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-mKate2
Plasmid#199249PurposeFluorescent labelling of bacterial cells with mKate2DepositorInsertCodon-optimized mKate2 gene
ExpressionBacterialMutationCodon optimization for XanthomonadaceaePromoterPc promoter from class III integron of Delftia a…Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
PKN3 gRNA (BRDN0001146586)
Plasmid#77947Purpose3rd generation lentiviral gRNA plasmid targeting human PKN3DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
NUAK1 gRNA (BRDN0001147733)
Plasmid#75576Purpose3rd generation lentiviral gRNA plasmid targeting human NUAK1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
NUAK1 gRNA (BRDN0001162525)
Plasmid#75578Purpose3rd generation lentiviral gRNA plasmid targeting human NUAK1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001149423)
Plasmid#80255Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-CD80-mGFP
Plasmid#223591PurposeLentiviral expression of human CD80 with mGFP tag in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
PHKG1 gRNA (BRDN0001144981)
Plasmid#76356Purpose3rd generation lentiviral gRNA plasmid targeting human PHKG1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PFKFB4 gRNA (BRDN0001149495)
Plasmid#77123Purpose3rd generation lentiviral gRNA plasmid targeting human PFKFB4DepositorAvailable SinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
STK17A gRNA (BRDN0001149480)
Plasmid#77916Purpose3rd generation lentiviral gRNA plasmid targeting human STK17ADepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
STK17B gRNA (BRDN0001146764)
Plasmid#76798Purpose3rd generation lentiviral gRNA plasmid targeting human STK17BDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PKN3 gRNA (BRDN0001146792)
Plasmid#77948Purpose3rd generation lentiviral gRNA plasmid targeting human PKN3DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PKN3 gRNA (BRDN0001146747)
Plasmid#77949Purpose3rd generation lentiviral gRNA plasmid targeting human PKN3DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PKN3 gRNA (BRDN0001487150)
Plasmid#77950Purpose3rd generation lentiviral gRNA plasmid targeting human PKN3DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMMDS DEST
Plasmid#206264PurposeDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. After LR propagate in Pir+. Works as a CRE-donor in the MultiBac system.DepositorTypeEmpty backboneUseCre-donor, multimate/gateway dest vector (multiba…Available SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV CAG ChR2 E123T T159C 2A tDimer
Plasmid#85399PurposeHigh efficiency channelrhodopsin with cytomegalovirus enhancer fused to chicken β-actin (CAG) promoterDepositorInsertsChannelrhodopsin-2
red fluorescent protein
UseAAVExpressionMammalianMutationE123T , T159CPromoterCAG and ribosomal skip sequence 2AAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSUC2::GNSI (NrsR)
Plasmid#121537PurposeThis plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.DepositorInsertsCYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator
NrsR
TagsmGFP5ExpressionYeastMutationM109I (Naturally occurring SNP) and The first 21 …Available SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-deSpCas9-VP64-6xHis
Plasmid#92117PurposeExpression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive eSpCas9-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, H840A, K848A, K1003A, R1060APromoterT7Available SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)
Plasmid#134331PurposeCAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tagDepositorInserthuman codon optimized Nme2Cas9
TagsNLS(SV40)-3xFLAGExpressionMammalianPromoterCAGAvailable SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.iGluSnFr.WPRE.SV40
Plasmid#98932PurposeCre dependent AAV expression of glutamate sensor from GFAP promoter ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVExpressionMammalianPromoterCAGAvailable SinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)
Plasmid#115142PurposeCAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tagDepositorInserthuman codon optimized Mb3Cas12a
TagsNLS(nucleoplasmin) and 3xHAExpressionMammalianAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE-LynG
Plasmid#206867PurposeExpression of LynG probe - LynAnchor-eGFPDepositorInsertLynG
UseRetroviralTagseGFPExpressionMammalianMutationContains Lyn amino acids 1-16Available SinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
Full human ELL2
Plasmid#127253PurposeExpresses full-length human ELL2 in mammalian cellsDepositorAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pAct-AtINTphiC31
Plasmid#127535PurposePlasmid encodes A. thaliana codon optimized Integrase phiC31.DepositorInsertIntegrase phiC31 coding sequence codon optimized for A. thaliana expression.
ExpressionPlantAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-shNCLX-2
Plasmid#181871PurposeExpresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoterDepositorInsertsolute carrier 8 family member B1 (Slc8b1 Mouse)
UseAAV, Adenoviral, and Mouse TargetingTagsmCherryExpressionMammalianPromoterU6Available SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-shNCLX-1
Plasmid#181870PurposeExpresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoterDepositorInsertsolute carrier 8 family member B1 (Slc8b1 Mouse)
UseAAV, Adenoviral, and Mouse TargetingTagsmCherryExpressionMammalianPromoterU6Available SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MCU-S259A
Plasmid#31728DepositorAvailable SinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-TetR
Plasmid#103813PurposeBLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorExpressionMammalianMutationTetR: A4G (M2V)PromoterCMVAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
ROCK2 gRNA (BRDN0001147800)
Plasmid#76349Purpose3rd generation lentiviral gRNA plasmid targeting human ROCK2DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TRIM27 gRNA (BRDN0001145893)
Plasmid#77516Purpose3rd generation lentiviral gRNA plasmid targeting human TRIM27DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pAct-AtINT13
Plasmid#127534PurposePlasmid encodes A. thaliana codon optimized Integrase 13.DepositorInsertIntegrase 13 coding sequence codon optimized for A. thaliana expression.
ExpressionPlantAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40
Plasmid#98931PurposeCre dependent AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVExpressionMammalianPromoterhSynAvailable SinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pROF141
Plasmid#155329PurposeBinary vector for expression of the PULSE system under the control of PAtUbi10 promoterDepositorInsertLB_PAtUbi10-SRDX-NLS-EL222-Tnos_SF_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB
UseSynthetic BiologyExpressionPlantAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E6MBW6
Plasmid#103102PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE6MBW6(Cas9 coding gene from Staphylococcus lugdunensis M23590)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
SEPHS2 gRNA (BRDN0001148921)
Plasmid#77661Purpose3rd generation lentiviral gRNA plasmid targeting human SEPHS2DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E3CY56
Plasmid#103075PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS313_ATP3T911A
Plasmid#120254PurposeExpression of ATP3-6 in yeastDepositorInsertATP3 (ATP3 Budding Yeast)
ExpressionYeastMutationT911 changed to A; Isoleucine 304 changed to Aspa…PromoterATP3Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001162235)
Plasmid#80261Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001149469)
Plasmid#80257Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
NEK10 gRNA (BRDN0001162335)
Plasmid#75636Purpose3rd generation lentiviral gRNA plasmid targeting human NEK10DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PHKA1 gRNA (BRDN0001144977)
Plasmid#77838Purpose3rd generation lentiviral gRNA plasmid targeting human PHKA1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pAct-AtINTBxb1
Plasmid#127536PurposePlasmid encodes A. thaliana codon optimized Integrase Bxb1.DepositorInsertIntegrase Bxb1 coding sequence codon optimized for A. thaliana expression.
ExpressionPlantAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
FKBP-Stx3-mCitrine
Plasmid#92428PurposeExpression of syntaxin-3 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.DepositorInsertStx3 (Stx3 Rat)
TagsFKBP (FK506 binding protein 12); allows for heter…ExpressionMammalianPromoterCMVAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only