We narrowed to 13,583 results for: TIM

1. pCAG-C1V1-TT-mRuby2-ST

   Plasmid

   #109124

   Purpose

   Cation channelrhodopsin C1V1 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   C1V1-TT-mRuby2-ST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDM4-GLY

   Plasmid

   #49808

   Purpose

   Encodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration.

   Depositor

   Mattheos Koffas

   Article

   Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

   Inserts

   phosphoglycerate kinase
   glyceraldehyde-3-phosphate dehydrogenase
   pyruvate dehydrogenase subunit E1
   dihydrolipoamide acetyltransferase
   dihydrolipoamide dehydrogenase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. YTIP-GFP

   Plasmid

   #42238

   Depositor

   William Pu

   Article

   von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

   Insert

   YTIP-GFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tom20-CIB-stop

   Plasmid

   #117243

   Purpose

   Light activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switch

   Depositor

   Robert Hughes

   Article

   Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

   Insert

   CIBN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. NLS-GI-VP16-IRES-NLS-GAL4DBD-FKBP-SMBiT-FKF1

   Plasmid

   #183402

   Purpose

   Dual expression of GI-VP16 and GAL4DBD-FKBP-SmBiT-FKF1 in the nucleus

   Depositor

   Alice Ting

   Article

   Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

   Insert

   GI-VP16-IRES-GAL4DBD-FKBP-SMBiT-FKF1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. KA2628_pGL4-UBCmin-luc2/Tbx3-B'

   Plasmid

   #124201

   Purpose

   Luciferase reporter driven by the Tbx3 enhancer

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Insert

   Tbx3 enhancer
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   UBCmin

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMOS009E: Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

   Plasmid

   #163062

   Purpose

   Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   QuasAr2
   

   Use

   Gateway entry vector

   Tags

   mOrange2

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-CMV-GFP-2A-oG-WPRE

   Plasmid

   #102984

   Purpose

   Lentivirus expressing eGFP and optimised Rabies Glycoprotein (oG)

   Depositor

   Marco Tripodi

   Article

   Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

   Inserts

   Enhanced Green Fluorescent Protein (eGFP)
   Optimised Rabies glycoprotein (oG)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXNubWtgate

   Plasmid

   #105080

   Purpose

   Yeast mating-based Split Ubiquitin Assay, positive control

   Depositor

   Wolf Frommer

   Article

   Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

   Type

   Empty backbone

   Use

   Tags

   NubI

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pErbB2-ECFP

    Plasmid

    #66945

    Purpose

    Mammalian expression of rat ErbB2 tagged with ECFP

    Depositor

    Bo van_Deurs, Mads Lerdrup

    Article

    Lerdrup et al J Cell Sci. 2006 Jan 1;119(Pt 1):85-95.

    Insert

    ErbB2 (Erbb2 Rat)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pMOS010: CheRiff-eGFP channelrhodopsin (plasma membrane)

    Plasmid

    #163044

    Purpose

    CheRiff-eGFP channelrhodopsin (plasma membrane)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    CheRiff
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDFBB-eGFP

    Plasmid

    #32550

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    egfp (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Mutation

    Promoter

    Consitutive lac

    Available since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLPCX roGFP2-Prx2(C172A)

    Plasmid

    #158228

    Purpose

    Genetically encoded fluorescent H2O2 sensor roGFP2-Prx2(C172A) for cytosolic expression in mammalian cells (for stable cell line generation)

    Depositor

    Tobias Dick

    Article

    Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

    Insert

    roGFP2-Prx2(C172A)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. NLS-GI-VP64-IRES-NLS-GAL4DBD-M13-SmBiT-FKF1

    Plasmid

    #183400

    Purpose

    Dual expression of GI-VP64 and GAL4DBD-M13-SmBiT-FKF1 in the nucleus

    Depositor

    Alice Ting

    Article

    Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

    Insert

    GI-VP64-IRES-GAL4DBD-M13-SmBiT-FKF1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFC331

    Plasmid

    #87843

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and argB selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    argB
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and native pro…

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. PE-P3-maize

    Plasmid

    #185611

    Purpose

    For plant prime editing in maize plants

    Depositor

    Jinxiao Yang

    Article

    Xu et al Nat Plants. 2022 Jan;8(1):45-52. doi: 10

    Insert

    3XFLAG-4XNLS-M-MLV-nCas9(H840A)-4XNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ZmUbi

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-ChETA-mRuby2-ST

    Plasmid

    #109126

    Purpose

    Cation channelrhodopsin ChETA fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ChETA-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA6a-kanMX6-Purg1

    Plasmid

    #39349

    Depositor

    Jurg Bahler

    Article

    Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

    Insert

    urg1 promoter (urg1, urg1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    kanMX6 module - reverse orientation

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pFA6a-natMX6-Purg1

    Plasmid

    #39350

    Depositor

    Jurg Bahler

    Article

    Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

    Insert

    urg1 promoter (urg1, urg1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    natMX6 module - reverse orientation

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

20. CMV-H3.3-YFP

    Plasmid

    #8693

    Depositor

    Kami Ahmad

    Article

    Janicki et al Cell 2004 Mar 5;116(5):683-98.

    Insert

    H3.3A (His3.3A Fly)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAG-eGtACR1-mRuby2-ST

    Plasmid

    #109136

    Purpose

    Anion channelrhodopsin GtACR1 fused to ER export signal, mRuby2 and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    eGtACR1-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. H2A-mTurquoise-RHOG-QL-deltaCAAX

    Plasmid

    #176096

    Purpose

    Constitutively active, nuclear localized RhoG

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    RHOG (RHOG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    QL

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA5/FRT/TO_LAMP1_STf

    Plasmid

    #182006

    Purpose

    CMV overexpression of SNAP-tag fast in the lysosome of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    LAMP1_STf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMET7-GAG-GW

    Plasmid

    #194634

    Purpose

    Gateway destination vector for mammalian expression of Virotrap bait constructs

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    p55 GAG
    

    Use

    Tags

    PQS1

    Expression

    Mammalian

    Mutation

    Promoter

    SRalpha

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1-RaACR_687-EYFP

    Plasmid

    #111077

    Purpose

    Anion channelrhodopsin from Rhodomonas abbreviata for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin RaACR_687
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-PxCas12a-2AeGFP

    Plasmid

    #123637

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    PxCas12a
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMET7-GAG-HRAS

    Plasmid

    #80604

    Purpose

    Mammalian expression vector that expresses the HIV-1 GAG protein with HRAS fused to it

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    HIV-1 p55 GAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SRalpha

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEB2-TagRFP

    Plasmid

    #103997

    Purpose

    Plasmid encoding TagRFP

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    TagRFP
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (R32G, K42R, K67R, …

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mNG-sfTq2

    Plasmid

    #124218

    Purpose

    Cytoplasmic mNeongreen superfolder mTurquoise2 tandem, ~60 % energy transfer. pNM044 - pSAV057-mNG-sfTq2

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

    Insert

    mNG-sfTq2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV MN (eGFP)

    Plasmid

    #89932

    Purpose

    Expresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

    Depositor

    Carl Novina

    Article

    Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

    Insert

    MN
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    M.SssI residues 2-272 fragment

    Promoter

    CMV

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge