4,132 results

1. GSK3 beta pGEX

   Plasmid

   #15898

   Depositor

   Jim Woodgett

   Insert

   GSK3 beta (GSK3B Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 1, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. CMV500 A-FOS

   Plasmid

   #33353

   Depositor

   Charles Vinson

   Article

   Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

   Insert

   FOS (Fos Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Dominant Negative of c-Jun; heterodimerizes and i…

   Promoter

   CMV

   Available since

   Dec. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-DN-hCUL3-FLAG

   Plasmid

   #15820

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005;399:287-309. doi:

   Insert

   Cullin3 (CUL3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N418

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET23b-Casp3-His

   Plasmid

   #11821

   Depositor

   Guy Salvesen

   Article

   Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

   Insert

   Caspase 3 (CASP3 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCSII-EF/mt-(n1)StayGold

   Plasmid

   #185823

   Purpose

   A soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.

   Depositor

   Atsushi Miyawaki

   Article

   Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

   Insert

   mt-(n1)StayGold
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PLKO.1-Scrambled

   Plasmid

   #136035

   Purpose

   Scrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   None (Scrambled)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MIGR1

   Plasmid

   #27490

   Depositor

   Warren Pear

   Article

   Pear et al Blood. 1998 Nov 15. 92(10):3780-92.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   GFP and IRES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mCh-alpha-tubulin

   Plasmid

   #49149

   Purpose

   expression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.

   Depositor

   Gia Voeltz

   Article

   Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

   Insert

   alpha tubulin (TUBA1B Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. T7-VEE-OKS-iM

   Plasmid

   #58972

   Purpose

   RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Inserts

   Oct4 (POU5F1 Human)
   Klf4 (KLF4 Human)
   Sox2 (SOX2 Human)
   cMyc (MYC Human)
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   26S subgenomic promoter and IRES

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-H2B-AID-EYFP

    Plasmid

    #47329

    Purpose

    for plasmid integration using the Flp-In system. AID at C-terminal

    Depositor

    Don Cleveland

    Article

    Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

    Insert

    histone H2B (H2BC21 Human)
    

    Use

    Tags

    AID and EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLifeAct_mScarlet_N1

    Plasmid

    #85054

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    LifeAct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-Xiap-Myc

    Plasmid

    #11833

    Depositor

    Guy Salvesen

    Article

    Takahashi et al J Biol Chem. 1998 Apr 3. 273(14):7787-90

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLentiCMV Puro DEST ERKKTRClover

    Plasmid

    #59150

    Purpose

    Lentiviral vector to express ERK KTR mClover under CMV promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    ERK Kinase Translocation Reporter
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENTR5'_ubi

    Plasmid

    #27320

    Depositor

    Leonard Zon

    Article

    Mosimann et al Development. 2011 Jan . 138(1):169-77.

    Insert

    zebrafish ubiquitin promoter (ubb Zebrafish)
    

    Use

    Multisite gateway 5' entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-hLbCpf1(TYCV) (pY230)

    Plasmid

    #89355

    Purpose

    Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1, RR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    G532R/K595R

    Promoter

    CMV

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLacO-ISce1

    Plasmid

    #58505

    Purpose

    provides 256 Lac operator repeats adjacent to the ISce1 restriction site

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    Lac repeats adjacent to 6 copies of the ISce1 restriction site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    no promoter

    Available since

    Oct. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBPGAL4.2Uw-2

    Plasmid

    #26227

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    GAL4 (GAL4 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. perbB2-EGFP

    Plasmid

    #39321

    Depositor

    Martin Offterdinger

    Article

    Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

    Insert

    erbB2 (ERBB2 Human)
    

    Use

    Tags

    egfp

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3/Flag-METTL14

    Plasmid

    #53740

    Purpose

    For expression of METTL14 (methyltransferase-like 14) in human cells

    Depositor

    Chuan He

    Article

    Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

    Insert

    Methyltransferase-like 14 (METTL14 Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SpyTag003-sfGFP

    Plasmid

    #133454

    Purpose

    Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-sfGFP
    

    Use

    Tags

    GSGSGS linker and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge