We narrowed to 23,602 results for: CRISPR

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pAN-PTet-dCas9

Plasmid

#62244

Purpose

controls the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

dCas9

Use

CRISPR

Expression

Bacterial

Mutation

catalytically inactive, D10A, H840A

Promoter

Ptet

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pMCB320

Plasmid

#89359

Purpose

3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites

Depositor

Michael Bassik

Article

Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

Insert

puromycin resistance, mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pJAT30

Plasmid

#204294

Purpose

For marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-DsRed

Use

CRISPR

Promoter

MHC

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pKEW321-MbCas12a-RFP-GFP-array

Plasmid

#115661

Purpose

Encodes an CRISPR array for MbCas12a targeting superfolder GFP and mRFP1

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

GFP and RFP array for MbCas12

Use

CRISPR

Expression

Bacterial

Promoter

Plac

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pTarget12f1

Plasmid

#171183

Purpose

Constitutive expression of sgRNA without donor DNA

Depositor

Kohsuke Honda

Article

Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

Insert

sgRNA scaffold in the CRISPR/Cas12f1 system

Use

CRISPR

Expression

Bacterial

Promoter

J23119(SpeI) promoter

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-Cas9-gRNA-TagBFP2

Plasmid

#124774

Purpose

3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-SpG

Plasmid

#211549

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

Insert

PEmax-SpG

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_mcherry

Plasmid

#167862

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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JME4580

Plasmid

#129659

Purpose

HPHex_CrisprCas9-yl_RFP: CAS9 vector with Hygromycinex marker for gRNA cloning using GoldenGate

Depositor

Tristan Rossignol

Article

Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCI152-pLenti-SiT-Cas12a

Plasmid

#128405

Purpose

Express both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST

Plasmid

#49771

Purpose

Level 1 hCas9 module

Depositor

Sophien Kamoun

Article

Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

Insert

35Sp::hCas9

Use

CRISPR

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_Puro

Plasmid

#167863

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCE048-SiT-Cas12a

Plasmid

#128124

Purpose

Express both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGG>nls-hCas9-nls

Plasmid

#99138

Purpose

Expresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryos

Depositor

Marianne Bronner

Article

Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

Insert

humanized Cas9

Use

CRISPR

Promoter

Chicken beta actin (CAG)

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKm-HABE

Plasmid

#170332

Purpose

a modularized single-plasmid adenosine base editing (A-to-G) tool for Sinorhizobium meliloti

Depositor

Deqiang Duanmu

Article

Wang et al Front Microbiol. 2021 Jun 18;12:686008.

Insert

Rhizobium codon-optimized ABEs

Use

CRISPR

Mutation

Cas9n-D10A

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-dCas9-p300_Blast

Plasmid

#192653

Purpose

3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

dCas9-p300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A; H840A in Cas9

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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NM9-SpCas9-NLS3

Plasmid

#128177

Purpose

Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

Depositor

Huimin Zhao

Article

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

SpCas9-NLS3

Use

CRISPR

Expression

Yeast

Promoter

pPGK1

Available Since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

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p201G Cas9

Plasmid

#59178

Purpose

Cas9 driven by double 35S, GFP for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
sGFP

Use

CRISPR

Promoter

2x35S

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pEF_dCas9

Plasmid

#68416

Purpose

Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A/H841A

Promoter

human EF1[alpha]

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pxpr_502-wt-ltr

Plasmid

#182647

Purpose

for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF, contains intact LTR

Depositor

Jakob Reiser

Article

Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

Insert

None

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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