12,610 results

1. pMK291 (mAID-mClover-Bsr)

   Plasmid

   #72829

   Purpose

   C-terminal tagging with mAID-mClover using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-mClover-Bsr
   

   Use

   CRISPR

   Tags

   mAID-mClover

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-Ran-Q69L-mRFP1-polyA

   Plasmid

   #104560

   Purpose

   Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Ran (Ran Mouse)
   

   Use

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   Q69L

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMiniT:SEP-GluN2B TKIT donor

   Plasmid

   #169431

   Purpose

   Donor DNA for TKIT knockin of SEP to NR2B in mouse

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. scFv-GCN4-DNMT3a(R887E)-DNMT3l

   Plasmid

   #154141

   Purpose

   Expresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.

   Depositor

   Albert Jeltsch

   Article

   Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

   Insert

   scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
   

   Use

   Tags

   HA and sfGFP

   Expression

   Mammalian

   Mutation

   R887E

   Promoter

   SFFV

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUSEss-CHIg-mG2a_M18

   Plasmid

   #105930

   Purpose

   Expression plasmid coding for heavy chain of mouse IgG2a antibody specific to antigen B of the ABO blood group system, clone M18.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

   Insert

   IgG2b heavy chain (Ighg2b Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hEF1-HTLV prom

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLAG-Foxo1ADA(pCMV5)

   Plasmid

   #12149

   Depositor

   Domenico Accili

   Article

   Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

   Insert

   Foxo1 ADA (Foxo1 Mouse)
   

   Use

   Tags

   Flag and Myc

   Expression

   Mammalian

   Mutation

   Threonine 24 to Alanine (A) and Serine 253 to Asp…

   Promoter

   CMV

   Available since

   June 13, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pH2B-miRFP720-N1

   Plasmid

   #197231

   Purpose

   Expresses the fusion protein of H2B-miRFP720 in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   H2B-miRFP720
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFLAG-GATA3

   Plasmid

   #1419

   Depositor

   Gokhan Hotamisligil

   Article

   Tong et al Mol Cell Biol 2005 Jan;25(2):706-15.

   Insert

   GATA3 (Gata3 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. mPlum-MyosinIIA-C-18

   Plasmid

   #55989

   Purpose

   Localization: Cytoskeleton, Excitation: 590, Emission: 649

   Depositor

   Michael Davidson

   Insert

   MyosinIIA (Myh9 Mouse)
   

   Use

   Tags

   mPlum

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Banshee Runx1FL mCherry

    Plasmid

    #80157

    Purpose

    retroviral expression of Runx1Fl and mCherry

    Depositor

    Ellen Rothenberg

    Insert

    Runx1 (Runx1 Mouse)
    

    Use

    Retroviral

    Tags

    HA and IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA

    Plasmid

    #156445

    Purpose

    For transient expression of mouse RAD21 (SCC1) tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse RAD21 (Rad21 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. psiCHECK2-2A-3xFLAG-XBP1u_pause-2A

    Plasmid

    #159583

    Purpose

    Luciferase reporter for ribosome pausing

    Depositor

    Shintaro Iwasaki

    Article

    Han et al Cell Rep. 2020 May 5;31(5):107610. doi:

    Inserts

    XBP1u pause site
    EIF2S3 5UTR
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLHCX-FLAG-mPKM2

    Plasmid

    #44239

    Depositor

    Lewis Cantley, Matthew Vander Heiden

    Article

    Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

    Insert

    Pyruvate Kinase M2 (Pkm Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2013

    Availability

    Academic Institutions and Nonprofits only

14. Anti-GABA(A)R, Epsilon [N413/67R]

    Plasmid

    #114478

    Purpose

    Mammalian Expression Plasmid of anti-GABA(A)R, Epsilon (Human). Derived from hybridoma N413/67.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-GABA(A)R, Epsilon (Homo sapiens) recombinant mouse monoclonal antibody (GABRE Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ad-FOXA2

    Plasmid

    #131581

    Purpose

    Expresses FOXA2 in mammalian cells

    Depositor

    Haiyan Xu

    Insert

    Forkhead box A2 (Foxa2 Mouse)
    

    Use

    Adenoviral

    Tags

    No

    Expression

    Mutation

    No

    Promoter

    CMV

    Available since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5FRT-EF-Pdgfrbeta-EGFPN

    Plasmid

    #66790

    Purpose

    Expression of murine PDGFRbeta tagged with GFP under the control of EF1a promoter

    Depositor

    Lotte Pedersen

    Article

    Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

    Insert

    PDGFRbeta (Pdgfrb Mouse)
    

    Use

    Tags

    C-term EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAVS1-TetOn-3XFLAG-firefly-beta-globin-control-AAVS1

    Plasmid

    #184395

    Purpose

    Donor plasmid for stable integration of firefly luciferase control reporter at AAVS1

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Firefly luciferase beta-globin
    

    Use

    CRISPR; Donor plasmid for hdr

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-On 3G

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.CMV.SV40.THBS4-HA.SV40(polyA)

    Plasmid

    #155193

    Purpose

    Expresses murine Thrombospondin-4 (THBS4) protein with HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-4 (Thbs4 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCW57-mcGAS-LL/RK-NLS/D307A

    Plasmid

    #185459

    Purpose

    Inducible nuclear cGAS with D307A mutation

    Depositor

    Shitao Li

    Article

    Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

    Insert

    cGAS
    

    Use

    Lentiviral

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    L157R, L159K, D307A

    Promoter

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. 8061-E33

    Plasmid

    #234270

    Purpose

    Gateway ORF Entry clone of mouse Smad3 with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    Smad3 (Smad3 Mouse)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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