Addgene

We narrowed to 16,061 results for: JUN

Showing: 4651 - 4700 of 16061 results

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E42_gControl_dTet_IRES_mTurquoise2

Plasmid

#189799

Purpose

Retroviral delivery of control guide RNA

Depositor

Ellen Rothenberg

Article

Hosokawa et al Immunity. 2018 Jun 19;48(6):1119-1134.e7

Insert

Luciferase gRNA

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPbaCas13b-EGFP-NLS

Plasmid

#191365

Purpose

Overexpression of NLS-dPbaCas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dPbaCas13b-EGFP-NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-IRES-puro-NLS-dPba5Cas13b-EGFP-NLS

Plasmid

#191364

Purpose

Overexpression of NLS-dPba5Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dPba5Cas13b-EGFP-NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPba4Cas13b-EGFP-NLS

Plasmid

#191363

Purpose

Overexpression of NLS-dPba4Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dPba4Cas13b-EGFP-NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-IRES-puro-NLS-dHgm6Cas13b-EGFP-NLS

Plasmid

#191366

Purpose

Overexpression of NLS-dHgm6Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dHgm6Cas13b-EGFP-NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-IRES-puro-NLS-dBba2Cas13b-EGFP-NLS

Plasmid

#191362

Purpose

Overexpression of NLS-dBba2Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dBba2Cas13b-EGFP-NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-IRES-puro-NLS-dPba3Cas13b-EGFP-NLS

Plasmid

#191361

Purpose

Overexpression of NLS-dPba3Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dPba3Cas13b-EGFP-NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_ABE‐NG‐loop

Plasmid

#187057

Purpose

ABEmax-SpCas9-NG-loop

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

ABE‐NG‐loop

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pElectra1-LaminA

Plasmid

#179482

Purpose

Nuclear envelope labeling with blue fluorescent protein Electra1

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra1-LaminA

Use

Tags

Electra1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)

Plasmid

#158771

Purpose

Expression of synaptotagmin 1 variant with self-cleaving C2 domain.

Depositor

Edwin Chapman

Article

Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

Synaptotagmin 1 1-NS3/4a (Syt1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Flexible linkers, ADVVCC/QMSY cleavage site

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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L0-AA008

Plasmid

#185754

Purpose

Level 0 loop assembly vector containing the Anthoceros agrestis EF1a promoter (pAaEF1a).

Depositor

Peter Szoevenyi

Article

Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

Insert

pAaEF1a (promoter)

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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L0-AA018

Plasmid

#185753

Purpose

Level 0 loop assembly vector containing the Anthoceros agrestis Tip1;1 promoter (pAaTip1;1).

Depositor

Peter Szoevenyi

Article

Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

Insert

pAaTip1;1 (promoter)

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET3a-FruK

Plasmid

#186256

Purpose

Overexpression of FruK for purification

Depositor

Liskin Swint-Kruse, Emile Van Schaftingen

Article

Veiga-da-Cunha et al Protein Expr Purif. 2000 Jun;19(1):48-52

Insert

1-phosphofructokinase (fruK E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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NK72

Plasmid

#176608

Purpose

To test macrophage engulfment initiation. Lacks the FcyR chain.

Depositor

Meghan Morrissey

Article

Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

CD86 (CD86 Human)

Use

Lentiviral

Tags

HA, SNAP-tag and mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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iLID-mRuby3-iTrkA

Plasmid

#136510

Purpose

Mammalian expression of iLID fused to mRuby3 and intracellular signaling domain of TrkA (aa 450-799)

Depositor

Bianxiao Cui

Article

Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

Insert

iLID mRuby3 iTrkA (Ntrk1 Avena sativa, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
iLID-mRuby3-iTrkB

Plasmid

#136511

Purpose

Mammalian expression of iLID fused to mRuby3 and intracellular signaling domain of TrkB (aa 455-822)

Depositor

Bianxiao Cui

Article

Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

Insert

iLID mRuby3 iTrkB

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsTNRC6A-delMI-MII_F

Plasmid

#146298

Purpose

Mammalian Expression of HsTNRC6A-delMI-MII

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A-delMI-MII (TNRC6A Human)

Use

Tags

Expression

Mammalian

Mutation

Five non silent mutations A339T, V417M, R934G, D1…

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK91 pHomL-TDH3p-Neon(gfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)

Plasmid

#179075

Purpose

Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp)-Link-SCS2tmh, an overexpressed (bright) marker for the endoplasmic reticulum

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TDH3p-Neon(gfp)-Link-SCS2tmh

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK02 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitKanR, AmpR)

Plasmid

#178986

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK66 pHomL-RPL18Bp-TOM70-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179050

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of TOM70-Link-Neon(gfp), a marker for the mitochondria

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-TOM70-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK65 pHomL-RPL18Bp-preCOX4-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179049

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of preCOX4-Link-Neon(gfp), a marker for the mitochondria

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-preCOX4-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK64 pHomL-RPL18Bp-PEX25-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179048

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PEX25-Link-Neon(gfp), a marker for the peroxisome

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PEX25-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK63 pHomL-RPL18Bp-PDR16-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179047

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PDR16-Link-Neon(gfp), a marker for the lipid droplet

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PDR16-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK62 pHomL-RPL18Bp-ERG6-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179046

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of ERG6-Link-Neon(gfp), a marker for the lipid droplet

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-ERG6-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK61 pHomL-RPL18Bp-Neon(gfp)-Link-ATG8-SSA1t-HomR (AmpR)

Plasmid

#179045

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-ATG8, a marker for the autophagosome

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-ATG8

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK60 pHomL-RPL18Bp-PRC1-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179044

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PRC1-Link-Neon(gfp), a marker for the vacuole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PRC1-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK59 pHomL-RPL18Bp-Neon(gfp)-Link-VAM3-SSA1t-HomR (AmpR)

Plasmid

#179043

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-VAM3, a marker for the vacuole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-VAM3

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK58 pHomL-RPL18Bp-Neon(gfp)-Link-YPT7-SSA1t-HomR (AmpR)

Plasmid

#179042

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-YPT7, a marker for the vacuole, late endosome

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-YPT7

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK57 pHomL-RPL18Bp-SNF7-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179041

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SNF7-Link-Neon(gfp), a marker for the endosome, class E compartment

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SNF7-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK56 pHomL-RPL18Bp-Neon(gfp)-Link-VPS21-SSA1t-HomR (AmpR)

Plasmid

#179040

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-VPS21, a marker for the endosomes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-VPS21

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK55 pHomL-RPL18Bp-PIL1-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179039

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PIL1-Link-Neon(gfp), a marker for the eisosomes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PIL1-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK53 pHomL-RPL18Bp-Neon(gfp)-Link-SFT2-SSA1t-HomR (AmpR)

Plasmid

#179037

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-SFT2, a marker for the late Golgi

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-SFT2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK52 pHomL-RPL18Bp-Neon(gfp)-Link-SED5-SSA1t-HomR (AmpR)

Plasmid

#179036

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-SED5, a marker for the early Golgi

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-SED5

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK50 pHomL-RPL18Bp-ARC35-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179034

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of ARC35-Link-Neon(gfp), a marker for the actin cytoskeleton

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-ARC35-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK48 pHomL-RPL18Bp-SPC42-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179032

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SPC42-Link-Neon(gfp), a marker for the spindle pole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SPC42-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK47 pHomL-RPL18Bp-SRM1-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179031

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SRM1-Link-Neon(gfp), a marker for the nucleus

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SRM1-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK46 pHomL-RPL18Bp-RPC19-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179030

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of RPC19-Link-Neon(gfp), a marker for the nucleolus

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-RPC19-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK14 pCAS-Tyr-[gRNA: 4=XII-5] (SplitKanR, AmpR)

Plasmid

#178998

Purpose

Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK13 pCAS-Tyr-[gRNA: 3=XII-2] (SplitKanR, AmpR)

Plasmid

#178997

Purpose

Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK10 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitURA3,AmpR)

Plasmid

#178994

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitURA3

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK09 pCAS-Tyr-[gRNA: BsaI GFP dropout] (BsmBI-flanked URA3, AmpR)

Plasmid

#178993

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = URA3

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK08 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitLEU2,AmpR)

Plasmid

#178992

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitLEU2

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK07 pCAS-Tyr-[gRNA: BsaI GFP dropout] (LEU2,AmpR)

Plasmid

#178991

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = LEU2

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK06 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitNatR,AmpR)

Plasmid

#178990

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitNourseothrycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-sg-tdTomato166/892

Plasmid

#179919

Purpose

Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 166 and 892.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

tdTomato sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-delMI-MII-AGOhook_F

Plasmid

#146299

Purpose

Mammalian Expression of HsTNRC6A-delMI-MII-AGOhook

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A-delMI-MII-AGOhook (TNRC6A Human)

Use

Tags

Expression

Mammalian

Mutation

Four non silent mutations A339T, R934G, D1289G, a…

Promoter

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-mScn8a-3xHA-Syn-smFP-flag

Plasmid

#182563

Purpose

For mouse Nav1.6 knockin with 3xHA at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a gRNA and 3xHA donor DNA
smFP-flag

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

EF1 and U6

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-mScn8a-HaloTag-V5-EF1-sfGFP

Plasmid

#182565

Purpose

For mouse Nav1.6 knockin with HaloTag-V5 at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a gRNA and HaloTag-V5 donor DNA
sfGFP

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

EF1 and U6

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-mScn8a-3xV5-EF1-smFP-flag

Plasmid

#182562

Purpose

For mouse Nav1.6 knockin with 3xV5 at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a gRNA and 3xV5 donor DNA
smFP-flag

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

EF1 and U6

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsAGO1_C

Plasmid

#146083

Purpose

Mammalian Expression of HsAGO1

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO1 (AGO1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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