161,641 results

1. SnapTag-Binder

   Plasmid

   #179142

   Purpose

   Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).

   Depositor

   Klaus Hahn

   Article

   Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

   Insert

   SspB
   

   Use

   Tags

   SNAP-tag

   Expression

   Mammalian

   Mutation

   Y11K/A15E, residue numbering from pdb: 1ou9

   Promoter

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pH6HTC_ENO1-Halo

   Plasmid

   #175330

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   ENO1 (ENO1 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. DHB-mVenus

   Plasmid

   #136461

   Purpose

   Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2

   Depositor

   Tobias Meyer, Sabrina Spencer

   Article

   Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10

   Insert

   DNA Helicase B (HELB Human)
   

   Use

   Lentiviral

   Tags

   mVenus

   Expression

   Mutation

   Promoter

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR_EF1a_flagLaM4-Notch-Gal4VP64

   Plasmid

   #216669

   Purpose

   Expresses anti-mCherry synNotch Gal4VP64

   Depositor

   Leonardo Morsut

   Insert

   anti-mCherry synNotch Gal4VP64
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGT-Kh2

   Plasmid

   #122587

   Purpose

   MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   beta-lactamase/sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDEST40-2XHA-Src-K298M

   Plasmid

   #140314

   Purpose

   Expresses HA-tagged human Src-K298M in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Use

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   K298M

   Promoter

   CMV

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ddo_piwi-1

   Plasmid

   #107628

   Purpose

   In situ hybridization and RNAi studies in planarians

   Depositor

   Alejandro Sánchez

   Article

   Accorsi et al The American Biology Teacher 79(3):208-2

   Insert

   piwi-1
   

   Use

   RNAi; In situ hybridization

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-NLS-CBRed-d2-hygro

   Plasmid

   #139196

   Purpose

   non-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puro

   Depositor

   Matthew Lazzara

   Article

   Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

   Insert

   CBRed
   

   Use

   Luciferase and Retroviral

   Tags

   d2 PEST domain and nuclear localization signal (N…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PIA586

   Plasmid

   #104399

   Purpose

   vector for expression of single E.coli RNAP subunit

   Depositor

   Irina Artsimovitch

   Article

   Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

   Insert

   rpoD
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFUW-TetO-Fos

    Plasmid

    #131593

    Purpose

    doxycycline-inducible expression of mouse c-Fos in mammalian cells

    Depositor

    Filipe Pereira, Kateri Moore

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    FBJ osteosarcoma oncogene (Fos Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDmDERusk

    Plasmid

    #213702

    Purpose

    Vector for red-light-induced bacterial gene expression

    Depositor

    Andreas Moeglich

    Article

    Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDmREDusk

    Plasmid

    #213701

    Purpose

    Vector for red-light-repressed bacterial gene expression

    Depositor

    Andreas Moeglich

    Article

    Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pACYC-TtCas6-4xcrRNA4.5

    Plasmid

    #127764

    Purpose

    For expression of T. thermophilus Csm complex in E. coli (crRNA)

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

    Inserts

    Synthetic CRISPR array containing four identical T. thermophilus spacers
    Cas6A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E. coli

    Promoter

    T7

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag-mCherry-Binder

    Plasmid

    #179139

    Purpose

    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    SspB
    

    Use

    Tags

    FLAG and mCherry

    Expression

    Mammalian

    Mutation

    Y11K/A15E, residue numbering from pdb: 1ou9

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40

    Plasmid

    #124603

    Purpose

    Cre-dependent soma-localized ChrimsonR for optogenetic stimulation

    Depositor

    Christopher Harvey

    Article

    Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

    Has Service

    AAV9

    Insert

    Soma-localized ChrimsonR
    

    Use

    AAV

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST40-2XFL-Src-K298M

    Plasmid

    #140315

    Purpose

    Expresses Flag-tagged human Src-K298M in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    K298M

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQCascade

    Plasmid

    #140622

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and CRISPR RNA

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pREDawn-StrR-MCS

    Plasmid

    #188979

    Purpose

    For red light-induced bacterial expression of gene of interest (StrR)

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pICH41421

    Plasmid

    #50339

    Depositor

    Nicola Patron, Sylvestre Marillonnet

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. C098

    Plasmid

    #174345

    Purpose

    Template for making Nluc mRNA reporter with pA139.

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    NanoLuc luciferase
    

    Use

    In vitro transcription template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge