163,584 results

1. pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2

   Plasmid

   #205161

   Purpose

   express β-ACTIN-3′UTR-24xMS2

   Depositor

   Weirui Ma

   Article

   Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

   Insert

   β-ACTIN-3′UTR-24xMS2
   

   Use

   Tags

   tagBFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

   Plasmid

   #103866

   Purpose

   dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Inserts

   PspCas13b
   huADAR2DD (ADARB1 Human)
   

   Use

   CRISPR

   Tags

   GSGGGGS and HIV NES

   Expression

   Mammalian

   Mutation

   Changed histidne 133 to alanine and histidine 105…

   Promoter

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hsyn-NE2m

   Plasmid

   #208686

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m in neurons

   Depositor

   Yulong Li

   Insert

   GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. T444T

   Plasmid

   #113081

   Purpose

   Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.

   Depositor

   Tibor Vellai

   Article

   Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

   Type

   Empty backbone

   Use

   RNAi

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRH030

   Plasmid

   #159633

   Purpose

   CRISPR‐Cas9 tool for the genetic engineering of Bacillus phages

   Depositor

   Robert Hertel

   Article

   Otte et al Lett Appl Microbiol. 2020 Jul 2. doi: 10

   Type

   Empty backbone

   Use

   CRISPR; Shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Constitutive tracrRNA

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

   Plasmid

   #83946

   Purpose

   Cas9-carrying vector with kanMX marker

   Depositor

   Irina Borodina

   Article

   Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-6xHis-SUMO-MarathonRT

   Plasmid

   #109029

   Purpose

   Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

   Depositor

   Anna Pyle

   Article

   Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

   Insert

   ERE_01490
   

   Use

   Tags

   6xHis-SUMO tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRRL_SRSF2_WT_mCherry

   Plasmid

   #84020

   Purpose

   expression of SRSF2 in mammalian cells

   Depositor

   Robert Bradley

   Article

   Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

   Insert

   SRSF2 (SRSF2 Human)
   

   Use

   Lentiviral

   Tags

   2TA-mCherry and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. phCMV-GALV-MTR

   Plasmid

   #163612

   Purpose

   Retro and Lentiviral transduction of primary human germinal center B cells

   Depositor

   Daniel Hodson

   Article

   Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

   Insert

   GaLV MTR envelope
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. anti-FLAG M2 light chain

    Plasmid

    #175358

    Purpose

    Mammalian expression vector for over-expression of anti-FLAG M2 light chain

    Depositor

    Joost Snijder

    Article

    Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

    Insert

    anti-FLAG M2 light chain
    

    Use

    Tags

    N-terminal secretion signal peptide (MEAPAQLLFLLL…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTW1169

    Plasmid

    #236583

    Purpose

    TRV1

    Depositor

    Steven Jacobsen

    Article

    Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

    Insert

    TRV1
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEMS-II-dCas13-APO1-YTH

    Plasmid

    #204767

    Purpose

    GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    dCas13-NLS-APO1-YTH-HA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1alpha

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBZS-YP_009702000

    Plasmid

    #198379

    Purpose

    Express YP_009702000 in bacterial cells.

    Depositor

    Takashi Ito

    Article

    Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

    Insert

    YP_009702000 (CVA-1_913R )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7-PEmax for IVT

    Plasmid

    #178113

    Purpose

    Template for in vitro transcription of PEmax

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax
    

    Use

    Template for in vitro transcription

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mutation

    Detailed in manuscript

    Promoter

    T7 (inactivated)

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-HIF1alpha-pcDNA3

    Plasmid

    #18949

    Depositor

    William Kaelin

    Article

    Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

    Insert

    Hypoxia inducible factor 1 alpha (HIF1A Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCW57.1_blasti

    Plasmid

    #194067

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

    Plasmid

    #103865

    Purpose

    Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    PspCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and HIV NES

    Expression

    Mammalian

    Mutation

    Contains the H133A and H1058A mutations which ina…

    Promoter

    EF1a

    Available Since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-PspCas13b-gRNA-Actb1216

    Plasmid

    #155368

    Purpose

    PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    PspCas13b gRNA targeting Actb 1216
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-nlsABACUS2-400n

    Plasmid

    #203726

    Purpose

    Gateway entry vector for the the nlsABACUS2-400n biosensor for (+)-Abscisic acid

    Depositor

    Alexander Jones

    Article

    Rowe et al Nat Plants. 2023 Jun 26. doi: 10.1038/s4

    Insert

    nlsABACUS2-400n
    

    Use

    Synthetic Biology

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. KW54_HART_ODC1

    Plasmid

    #207398

    Purpose

    Lentiviral plasmid containing DDX3X reporter HART for ODC1 5UTR

    Depositor

    Stephen Floor

    Article

    Wilkins et al RNA. 2024 Jul 16;30(8):1041-1057. doi: 1

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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