We narrowed to 6,558 results for: siae
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Plasmid#200227Purposeuniversal template to build tRNA-gRNA array for multiplex editing CREEPYDepositorInserttRNA-gRNA scaffold
UseSynthetic BiologyAvailable SinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPM28 (eroGFP CEN/ARS URA3)
Plasmid#20131DepositorInserteroGFP (KAR2 Budding Yeast, jellyfish)
ExpressionYeastAvailable SinceFeb. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMLS260
Plasmid#73685PurposePunc-17::2xNLS-FLP-D5 Expression vectorDepositorInsertPunc-17::2xNLS-FLP-D5::let-858 3'-UTR
UseFlp/frtExpressionWormMutationG5DPromoterPunc-17Available SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMLS360
Plasmid#73687PurposePunc-47::2xNLS-FLP-D5 Expression vectorDepositorInsertPunc-47::2xNLS-FLP-D5::let-858 3'-UTR
UseFlp/frtExpressionWormPromoterPunc-47Available SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-L
Plasmid#51226Purposebi-directional expression vector for yeast, constitutively active, LEU2 selectionDepositorTypeEmpty backboneExpressionYeastPromoterGPD/ADH1Available SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::HIS3
Plasmid#227596PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::HIS3
Tags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL06
Plasmid#104430Purposesuper Ecliptic pHluorin with C terminal mRuby2 fusion tag in yeast expression vectorDepositorInsertSuper Ecliptic pHluorin-mRuby2
ExpressionYeastPromoterTef1Available SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH136
Plasmid#47554PurposepJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.DepositorInsertURA3
UsePcr templatePromoterNative URA3 promoter, -243 to -1.Available SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoTagRFP-T-Kan
Plasmid#44906PurposeYeast tagging vector to create gene fusion with yeast optimized TagRFP-T with Kan selectionDepositorInsertyoTagRFP-T
ExpressionYeastAvailable SinceOct. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYZ462
Plasmid#187970PurposeTEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4tDepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyExpressionYeastPromoterTEF1Available SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDest17_FL Gcn4
Plasmid#231855PurposeBacterial expression of N-terminally 6His tagged FL Gcn4DepositorInsertGcn4
Tags6xHis, TEV cleavage siteExpressionBacterialPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pYZ960
Plasmid#187972PurposeTEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2µ-URA3DepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyTagsSV40ExpressionYeastPromoterTEF1Available SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-U
Plasmid#51230Purposebi-directional expression vector for yeast, constitutively active, URA3 selectionDepositorTypeEmpty backboneExpressionYeastPromoterGPD/ADH1Available SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
FRP718_PACT1(-1-520)-LexA-ER-haB42-TCYC1
Plasmid#58431PurposeExpresses LexA-ER-B42 in yeast cellsDepositorInsertLexA-ER-B42
ExpressionYeastPromoterAct1 promoter (-520;1)Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-HALO-Phleomycin
Plasmid#188930PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-HALO-Phleomycin
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
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CF-Inp(D281A)
Plasmid#20156DepositorInsertCFP-FKBP-Inp54p (D281A) (INP54 Budding Yeast)
ExpressionMammalianMutationStop codon at position 331. D281A mutation.Available SinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
LT1_36_QUEEN-2m
Plasmid#177708PurposePlasmid for genome integration in X2 site expressing QUEEN-2m (ATP biosensor)DepositorInsertpTEF1-QUEEN2m
UseUsed as donor dna in genome integration after lin…ExpressionYeastAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHLUK
Plasmid#64181Purposeharbours marker genes HIS3, LEU2, URA3 and LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYES2-Ub-R-GFP
Plasmid#11953DepositorInsertUb-R-GFP
ExpressionYeastMutationUbiquitin fused to N-terminus of GFP. Arginine at…Available SinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHLUM (version 2)
Plasmid#64166Purposeharbours marker genes HIS3, LEU2, URA3 and MET17DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-yoHaloTag-TLG1
Plasmid#105277PurposePlasmid for pop-in/pop-out replacement of Tlg1 with yoHaloTag-Tlg1.DepositorInsertTlg1 (TLG1 Budding Yeast)
TagsyoHaloTagExpressionYeastMutationHaloTag has yeast optimized codonsAvailable SinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-GL
Plasmid#51225Purposebi-directional expression vector for yeast, galactose inducible, LEU2 selectionDepositorTypeEmpty backboneExpressionYeastPromoterGAL1,10Available SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
FRP795_insul-(lexA-box)8-PminCYC1-Citrine-TCYC1
Plasmid#58435PurposeInduces Citrine expression from a promoter containing 8 lexA boxes in yeast cellsDepositorInsertCitrineA206K
ExpressionYeastPromoterinsul-(lexA-box)8-PminCYC1Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::TRP1
Plasmid#227593PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::TRP1
Tags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKate2-SpHis5
Plasmid#44852PurposeYeast tagging vector to create gene fusion with yeast optimized mKate2 with SpHis5 selectionDepositorInsertyomKate2
ExpressionYeastAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDHP_YEAST
Plasmid#204233PurposeBacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHP_YEAST (MDH3 Budding Yeast)
Tags6X HisExpressionBacterialMutationThe last 3 C-terminal residues (SKL) are the pero…PromoterLacAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCCL-GKL1RC-Short-LEUg1mKateg2GKL2ORF10UCS
Plasmid#52345PurposeIntegration cassette to create p1-Short-Leu-mKate with p2 ORF10 UCS>mKateDepositorInsertmKate2
UseP1 integration vectorExpressionBacterialMutationchanged V2HPromoterpGKL2 ORF10 UCSAvailable SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHES553 [prGal1-RAS2(KAN) pnh604]
Plasmid#103846PurposeYeast Expression PlasmidDepositorInsertpGal1-Ras2(KAN)
ExpressionYeastAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomCherry-SpHis5
Plasmid#44841PurposeYeast tagging vector to create gene fusion with yeast optimized mCherry with SpHis5 selectionDepositorInsertyomCherry
ExpressionYeastAvailable SinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoEGFP-SpHis5
Plasmid#44836PurposeYeast tagging vector to create gene fusion with yeast optimized EGFP with SpHis5 selectionDepositorInsertyoEGFP
ExpressionYeastAvailable SinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA-GFP-Ubc9ts
Plasmid#20369DepositorInsertSUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)
UseYeast/bacteria shuttleTagsGFPExpressionYeastMutationY68L This mutation renders Ubc9 temperature-sens…Available SinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pESC-MET
Plasmid#222482PurposeCo-expression of two genes in yeast with FLAG and MYC tags and MET15 selectionDepositorTypeEmpty backboneTagsFLAG and MycExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-LYS
Plasmid#222483PurposeCo-expression of two genes in yeast with FLAG and MYC tags and LYS2 selectionDepositorTypeEmpty backboneTagsFLAG and MycExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dhh1
Plasmid#201412PurposeBacterial protein expression of Dhh1DepositorAvailable SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFM689
Plasmid#74635Purposealias pFA6a-mCherry-hphNT1 - vector for C-terminal tagging of yeast genes with mCherry and hygromycin selection markerDepositorInsertsExpressionYeastPromoternoneAvailable SinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-ABP1-iGFPx6
Plasmid#177099PurposeExpresses Abp1-iGFPx6 in yeast cellsDepositorInsertABP1 (ABP1 Budding Yeast)
ExpressionYeastAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PTEF1-MCP-no_intron
Plasmid#127597Purposelow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoterDepositorInsert1xFLAG-MCP expression cassette
ExpressionYeastAvailable SinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomEos2-Kan
Plasmid#44944PurposeYeast tagging vector to create gene fusion with yeast optimized mEos2 with Kan selectionDepositorInsertyomEos2
ExpressionYeastAvailable SinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only