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We narrowed to 24,790 results for: Spr

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Showing: 1901 - 1920 of 24790 results

  2. pHSN6A01

    Plasmid
    #50586
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    2×35Sp and AtU6-26p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50586-plasmid-map-sequence-id-258465

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  3. pIA33

    Plasmid
    #120803
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Inserts
    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Base-pairing region targets red fluorescent prote…
    Promoter
    Pgdh and Pxyl
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120803-plasmid-map-sequence-id-235316

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  4. iCas

    Plasmid
    #84232
    Purpose
    Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)
    Depositor
    Meng How Tan
    Article
    Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038
    Insert
    Cas9
    Tags
    2A-OFP co-expression and ERT2-ERT2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    84232-plasmid-map-sequence-id-323254

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  5. pHSN6I01

    Plasmid
    #50587
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-KRAB
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 that is defective in DNA cleavage; the maiz…
    Promoter
    2×35Sp and AtU6-26p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50587-plasmid-map-sequence-id-75659

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  6. dCas9-ddMSK1

    Plasmid
    #165603
    Purpose
    Expresses Sp dCas9 fused to truncated inactive human MSK1 (42-802, D195A, D565A)
    Depositor
    Isaac Hilton
    Article
    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1
    Insert
    S. Pyogenes dCas9 with c-terminal truncated inactive human Mitogen- and stress-activated protein kinase-1 (42-802, D195A, D565A) (RPS6KA5 Human, Synthetic, S. Pyogenes)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    165603-plasmid-map-sequence-id-327022

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  8. lenti SYN-SVI-DIO-dCas9-VPR

    Plasmid
    #164576
    Purpose
    Expresses a SYN-driven, intron-containing dCas9-VPR fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.
    Depositor
    Jeremy Day
    Article
    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21
    Insert
    A DIO FLAG-dCas9-VPR cassette containing an SV40 intron
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Available Since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    164576-plasmid-map-sequence-id-323657

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  12. pBUN6A11

    Plasmid
    #50579
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    OsU3p and Ubi1p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50579-plasmid-map-sequence-id-75626

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  13. pBUN6I11

    Plasmid
    #50580
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-KRAB
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 that is defective in DNA cleavage; the maiz…
    Promoter
    OsU3p and Ubi1p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50580-plasmid-map-sequence-id-75637

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  15. U6_sgRNA_CAG_hSt1Cas9_LMD9

    Plasmid
    #110626
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and hU6
    Available Since
    May 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    110626-plasmid-map-sequence-id-208002

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  16. pY036_ATP1A1_G5_Array

    Plasmid
    #86620
    Purpose
    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G5 crRNA + user-specified crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86620-plasmid-map-sequence-id-168857

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  17. pY036_ATP1A1_G3_Array

    Plasmid
    #86619
    Purpose
    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via nhej using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86619-plasmid-map-sequence-id-168855

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Showing: 1901 - 1920 of 24790 results