We narrowed to 14,145 results for: TIM
-
Plasmid#209447Purposeimproved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoterDepositorInsertCodon optimized APOBEC1-nCas9-UGI fusion protein
UseCRISPR and Synthetic BiologyPromoterPtipA; sgRNA expression from kasOP* promoterAvailable SinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro
Plasmid#189633PurposeLentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs librariesDepositorInserth7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette
UseCRISPR and LentiviralExpressionMammalianAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2
Plasmid#189632PurposeLentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs librariesDepositorInserth7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette
UseCRISPR and LentiviralExpressionMammalianAvailable SinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-6xHis-TEV-ORF9c
Plasmid#201023PurposeBacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.DepositorInsertopen reading frame 9c
Tags6xHis-TEVExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-HiBIT-WDR5
Plasmid#200883PurposeBacterial expression of N-terminal HiBIT-tagged human WDR5 proteinDepositorAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pShuttle.CC9
Plasmid#192931PurposeGateway compatible vector with BsaI cloning site and Cre-2A-Cas9 expressionDepositorTypeEmpty backboneUseGateway-compatible cloning vectorPromoterCMVAvailable SinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_R1CiM_4EGFP_R3EGFPi2
Plasmid#185968PurposeMolecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.DepositorInsertMolecular IMPLY gate
UseSynthetic BiologyAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-NLP-cMyc AspCas12a
Plasmid#182120PurposepET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteriaDepositorInsert2xNLS-NLP-cMyc AspCas12a
Tags6xHisExpressionBacterialPromoterT7Available SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ATG13 (1-197)
Plasmid#188074PurposeTo make ATG101/ATG13 HORMA complex for expression in mammalian cellsDepositorAvailable SinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-MMETn-del-gRNA2
Plasmid#185007Purposeexpression of gRNA and Cas9 from S. pyogenes with 2A-EGFPDepositorInsertMMETn-del-gRNA2
UseCRISPRAvailable SinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-IAP-del-gRNA1
Plasmid#185002Purposeexpression of gRNA and Cas9 from S. pyogenes with 2A-EGFPDepositorInsertIAP-del-gRNA1 (Akp3 Mouse)
UseCRISPRAvailable SinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-MMERVK9C-del-gRNA1
Plasmid#185004Purposeexpression of gRNA and Cas9 from S. pyogenes with 2A-EGFPDepositorInsertMMERVK9C-del-gRNA1
UseCRISPRAvailable SinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-MMETn-del-gRNA1
Plasmid#185006Purposeexpression of gRNA and Cas9 from S. pyogenes with 2A-EGFPDepositorInsertMMETn-del-gRNA1
UseCRISPRAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-MMERVK9C-del-gRNA2
Plasmid#185005Purposeexpression of gRNA and Cas9 from S. pyogenes with 2A-EGFPDepositorInsertMMERVK9C-del-gRNA2
UseCRISPRAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-IAP-del-gRNA2
Plasmid#185003Purposeexpression of gRNA and Cas9 from S. pyogenes with 2A-EGFPDepositorInsertIAP-del-gRNA2 (Akp3 Mouse)
UseCRISPRAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
TOMM20-mTurquoise-RhoA-G14V-deltaCaaX
Plasmid#176123PurposeConstitutively active, mitochondrial localized RhoADepositorAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS313_ATP3G919C
Plasmid#120255PurposeExpression of ATP3-7 in yeastDepositorInsertATP3 (ATP3 Budding Yeast)
ExpressionYeastMutationG919 changed to C; Alanine 307 changed to ProlinePromoterATP3Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS313_ATP3mut
Plasmid#120256PurposeExpression of ATP3-67 in yeastDepositorInsertATP3 (ATP3 Budding Yeast)
ExpressionYeastMutationIsoleucine 304 changed to Asparagine; Alanine 307…PromoterATP3Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBI-CMV1-mir144~451-CMV2-mir1
Plasmid#164664PurposeExpress miR-144~451 and miR-1-1 in mammalian cellsDepositorExpressionMammalianPromoterCMVAvailable SinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA™5/FRT/CMV_promoter-ciCas9-E2A-mRFP
Plasmid#164132PurposeExpresses ciCas9 and mRFP in mammalian cells (an insertion vector to generate ciCas9-knockin cell lines)DepositorInsertciCas9
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterCMVAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKM33
Plasmid#154167PurposeAedes aegypti Rel2 (long isoform) with C-terminal IκB domain deleted, expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.DepositorInsertRel2 (long isoform)
TagsFLAGExpressionInsectMutationC-terminal IκB domain deletedPromoterIE1 promoter and hr5 enhancer from Autographa cal…Available SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pUB-HspINT4
Plasmid#127513PurposePlasmid encodes H. sapiens codon optimized Integrase 4.DepositorInsertIntegrase 4 coding sequence codon optimized for H. sapiens expression.
ExpressionMammalianAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pUB-HspINT5
Plasmid#127514PurposePlasmid encodes H. sapiens codon optimized Integrase 5.DepositorInsertIntegrase 5 coding sequence codon optimized for H. sapiens expression.
ExpressionMammalianAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1(CTD)-NLS(SV40) (KAC438)
Plasmid#133796PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 (truncated C-terminal domain only) with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA1 (truncated C-terminal domain only)
TagsNLS(SV40)ExpressionMammalianMutationC-terminal domain onlyPromoterCMV and T7Available SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1(T114A/F115A)-NLS(SV40) (KAC688)
Plasmid#133798PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T114A/F115A) with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA1(T114A/F115A)
TagsNLS(SV40)ExpressionMammalianMutationT114A/F115APromoterCMV and T7Available SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRTTO-NHASt-codoptUL33
Plasmid#135103PurposeCodon-optimized HSV-1 UL33 with N-terminal HAStrep tagDepositorInsertHSV-1 UL33 codon optimized
TagsHA-Strep-StrepExpressionMammalianAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRTTO-codoptUL50-CStHA
Plasmid#135105PurposeCodon-optimized HSV-1 UL50 with C-terminal StrepHA tagDepositorInsertHSV-1 UL50 codon optimized
TagsStrep-Strep-HAExpressionMammalianAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRTTO-codoptUL33-CStHA
Plasmid#135106PurposeCodon-optimized HSV-1 UL33 with C-terminal StrepHA tagDepositorInsertHSV-1 UL33 codon optimized
TagsStrep-Strep-HAExpressionMammalianAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRTTO-codoptUS2-CStHA
Plasmid#135107PurposeCodon-optimized HSV-1 US2 with C-terminal StrepHA tagDepositorInsertHSV-1 US2 codon optimized
TagsStrep-Strep-HAExpressionMammalianAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAWp63-clone3-12 (OptiHF-SpCas9)
Plasmid#131737PurposeExpresses OptiHF-SpCas9 in mammalian cellsDepositorInsertOptiHF-SpCas9
UseLentiviralTagsFlagMutationQ695A+K848A+E923M+T924V+Q926APromoterEFSAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
GS2.1/EC
Plasmid#132568PurposesgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt).DepositorInsertegg cell promoter, Cas9-mApple, pds3 sgRNA
UseCRISPR and Synthetic BiologyExpressionPlantPromoterA. thaliana egg cell promoterAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met133I, M1381I, M186I ELL2
Plasmid#127263PurposeFor in vitro translation of human ELL2 with Met133I, M1381I, M186IDepositorInsertELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
UseIn vitro translationMutationThree Mets (133, 138, 186) to IleuPromoterT7Available SinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
563aa ELL2 pEF4B
Plasmid#127273PurposeExpresses 563aa human WT ELL2DepositorInsertELL2 truncated at SphI (ELL2 Human)
ExpressionMammalianMutationContains 564 N-terminal amino acids ELL2, termina…PromoterpEF1aAvailable SinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_Ab-PirA
Plasmid#128959Purposeexpresses Acinetobacter baumannii PirA in E. coliDepositorInsertferric enterobactin receptor PirA
TagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removedPromoterT7Available SinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Designed HEL-binding scFv D44.1des
Plasmid#111719PurposeYeast Surface Display of D44.1desDepositorInsertD44.1 des
Tagscmyc tagExpressionYeastMutationL Q89G, L S55V, L S43P, H V37M, H H43G, H W47Y, H…Available SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged Sph ELL2
Plasmid#127270PurposeFor in vitro translation of human WT ELL2 with internal HA tagDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationMutationWT Mets and HA tag at SphI site (8th Met)PromoterT7Available SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged mid ELL2
Plasmid#127274PurposeExpresses human ELL2 with HA tag in the middle that does not disrupt functionDepositorInsertELL2 with HA at XbaI (ELL2 Human)
ExpressionMammalianMutationHA inserted at XbaI after Met 186PromoterpEF1aAvailable SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met138ILeu ELL2
Plasmid#127260PurposeFor in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met138 to Ileu) (ELL2 Human)
UseIn vitro translationMutationMet138 to Ileu QuikchangePromoterT7Available SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met186ILeu ELL2
Plasmid#127261PurposeFor in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met186 to Ileu) (ELL2 Human)
UseIn vitro translationMutationMet186 to Ileu QuikchangePromoterT7Available SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met138ILeu, Met186ILeu ELL2
Plasmid#127265PurposeFor in vitro translation of human ELL2 with Met138ILeu, Met186ILeuDepositorInsertELL2 (Met138ILeu, Met186ILeu) (ELL2 Human)
UseIn vitro translationMutationTwo Mets (138,186) to ILeu QuikchangePromoterT7Available SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met133ILeu ELL2
Plasmid#127259PurposeFor in vitro translation of human ELL2 with Met133 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met133 to Ileu) (ELL2 Human)
UseIn vitro translationMutationMet133 to Ileu QuikchangePromoterT7Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met133ILeu, Met186ILeu ELL2
Plasmid#127264PurposeFor in vitro translation of human ELL2 with Met133ILeu, Met186ILeuDepositorInsertELL2 (Met133ILeu, Met186ILeu) (ELL2 Human)
UseIn vitro translationMutationTwo Mets (133,186 )to ILeu QuikchangePromoterT7Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met331Leu ELL2
Plasmid#127262PurposeFor in vitro translation of human ELL2 with Met331 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met331 to Ileu) (ELL2 Human)
UseIn vitro translationMutationMet331 to ILeu QuikchangePromoterT7Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
COOHend ELL2
Plasmid#127255PurposeExpresses the C terminal segment of human ELL2 in mammalian cellsDepositorInsertELL2 (C-terminal end) (ELL2 Human)
UseOtherExpressionMammalianMutationcontains only the last 389 aaPromoterpEF1aAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met1ILeu ELL2
Plasmid#127258PurposeFor in vitro translation of human ELL2 with Met1 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met1 to Ileu) (ELL2 Human)
UseIn vitro translationMutationMet1 to Ileu by QuikchangePromoterT7Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
ELL2mRNA+polyA
Plasmid#127256PurposeFor in vitro translation of human ELL2 with 30 nt pA tailDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationMutationshortens 3'UTR to 40 bp adds A30 tailPromoterT7Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
NH2end ELL2
Plasmid#127252PurposeExpresses the N terminal segment of human ELL2 in mammalian cellsDepositorInsertELL2 (1-287) (ELL2 Human)
UseOtherTagsHis6XExpressionMammalianMutationcontains only amino acids 1-289PromoterpEF1aAvailable SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged Xba ELL2
Plasmid#127268PurposeFor in vitro translation of WT ELL2 with HA tag inserted near N-termDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationMutationELL2mRNA+polyA with HA tag inserted at aa12 betwe…PromoterT7Available SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-mCherry-CHMP2B-siRNAres
Plasmid#115333Purposeexpresses siRNA-resistant mCherry-CHMP2B in mammalian cellsDepositorInsertCHMP2B (CHMP2B Human)
UseRetroviralTagsmCherryExpressionMammalianMutationmutated to be resistant to siRNA knockdownPromoterCMVAvailable SinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-B2KB46
Plasmid#103086PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertB2KB46(Cas9 coding gene from Elusimicrobium minutum (strain Pei191))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only