We narrowed to 9,416 results for: Coli

1. pBsacA

   Plasmid

   #158650

   Purpose

   Constitutive transcription of sacA crRNA

   Depositor

   Wenjing Cui

   Insert

   sacA crRNA
   

   Use

   CRISPR and Synthetic Biology; Shuttle vector baci…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pveg

   Available Since

   Oct. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pExp-GST

   Plasmid

   #112566

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8xHis-GST and Strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHTganA-Cpf1

   Plasmid

   #158647

   Purpose

   Constitutive transcription of FnCpf1 and ganA crRNA

   Depositor

   Wenjing Cui

   Insert

   Cpf1, ganA crRNA
   

   Use

   CRISPR and Synthetic Biology; Shuttle vector baci…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P43

   Available Since

   Oct. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pACYC-LIC+

   Plasmid

   #62312

   Purpose

   E. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage site

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   6xHis and thrombin protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pADsacA

   Plasmid

   #158649

   Purpose

   Constitutive transcription of sacA crRNA and GFP

   Depositor

   Wenjing Cui

   Insert

   sacA crRNA
   

   Use

   CRISPR and Synthetic Biology; Shuttle vector baci…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pveg

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pACYC-GST

   Plasmid

   #62329

   Purpose

   E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   Glutathione S-transferase (GST) tag and TEV prote…

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lac0

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pExp-NHis

   Plasmid

   #112558

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8xHis and Strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOPINEneo-3C-GFP

   Plasmid

   #53534

   Purpose

   pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system

   Depositor

   Ray Owens

   Insert

   GFP
   

   Use

   Tags

   GFP-His8

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available Since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNIC28-Bsa4-iLOVf

   Plasmid

   #63722

   Purpose

   LIC E coli expression plasmid to produce TEV cleavable His tagged iLOV fusion proteins

   Depositor

   Brian Smith

   Type

   Empty backbone

   Use

   Tags

   TEE, iLOV, 6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Sc FLAG-S6-rad24-pRSFDuet

    Plasmid

    #240734

    Purpose

    Over-express FLAG-S6-rad24 in E. coli

    Depositor

    Michael O'Donnell

    Insert

    FLAG-S6-rad24 (RAD24 Budding Yeast)
    

    Use

    Tags

    3X FLAG followed by S6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. Sc [ddc1 + rad17]-pET

    Plasmid

    #240740

    Purpose

    Over-express Sc ddc1 & Sc rad17 in E. coli

    Depositor

    Michael O'Donnell

    Inserts

    ddc1 (DDC1 Budding Yeast)
    rad17 (RAD17 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Sc Rad27-S6- pET24

    Plasmid

    #239205

    Purpose

    Overexpress Sc Rad27 (FEN1) (C-terminal S6 tag) in E. coli

    Depositor

    Michael O'Donnell

    Insert

    Rad27 (RAD27 Budding Yeast)
    

    Use

    Tags

    S6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Sc FLAG-RFC1-pRSFDuet

    Plasmid

    #239201

    Purpose

    Overexpress Sc RFC1 (N-terminal 3X FLAG tag) in E. coli

    Depositor

    Michael O'Donnell

    Insert

    RFC1 (RFC1 Budding Yeast)
    

    Use

    Tags

    3X FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAIP4_1YW5

    Plasmid

    #234235

    Purpose

    Heterologous protein expression of peptidyl-prolyl cis-trans isomerase in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1YW5 (ESS1 Candida albicans)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pAIP4_4HS5

    Plasmid

    #234223

    Purpose

    Heterologous protein expression of iron-sulfur cluster assembly protein CyaY in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    4HS5
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pAIP4_1UKF

    Plasmid

    #234214

    Purpose

    Heterologous protein expression of cysteine protease avirulence protein AvrPphB in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1UKF
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pAIP4_1LU4

    Plasmid

    #234207

    Purpose

    Heterologous protein expression of soluble secreted antigen MPT53 in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1LU4 (mpt53 Mycobacterium tuberculosis)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pAIP4_1KAF

    Plasmid

    #234209

    Purpose

    Heterologous protein expression of middle transcription regulatory protein motA in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1KAF (motA Tequatrovirus T4)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pAIP4_1TP9

    Plasmid

    #234200

    Purpose

    Heterologous protein expression of peroxiredoxin-2 (Prx) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1TP9 (LOC7476296 Populus trichocarpa)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pAIP4_5H28

    Plasmid

    #234184

    Purpose

    Heterologous protein expression of oxysterol-binding protein homolog 1 in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    5H28 (SWH1 Budding Yeast)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pAIP4_4RZ9

    Plasmid

    #234164

    Purpose

    Heterologous protein expression of pre-mRNA-splicing factor 38A in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    4RZ9 (PRPF38A Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pAIP4_5YPV

    Plasmid

    #234150

    Purpose

    Heterologous protein expression of malonyl CoA-acyl carrier protein transacylase in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    5YPV
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pAIP4_1H6T

    Plasmid

    #234146

    Purpose

    Heterologous protein expression of internalin B (InlB) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1H6T (inlB Listeria monocytogenes)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pHL 1904

    Plasmid

    #53021

    Purpose

    AmpR + fimS::gfpLVA::Asp terminator + CamR + ColE1

    Depositor

    Han Lim

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Asp terminator, LVA, and fimS

    Expression

    Bacterial

    Mutation

    Promoter

    fimS

    Available Since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pHL 1977

    Plasmid

    #53025

    Purpose

    AmpR + PLtetO-1::RBS (st3) fimB::Asp terminator + ColE1

    Depositor

    Han Lim

    Insert

    fimB
    

    Use

    Synthetic Biology

    Tags

    RBS(st3)

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO-1

    Available Since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

26. StarShip01

    Plasmid

    #164505

    Purpose

    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains T7-HaloTag-PDGFR_TM-T2A-Puro.

    Depositor

    Bo Huang

    Type

    Empty backbone

    Use

    Bxbi site specific integration

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. StarShip02

    Plasmid

    #164506

    Purpose

    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES(Myb)-T7-Halo-PDGFR_TM-T2A-Puro.

    Depositor

    Bo Huang

    Type

    Empty backbone

    Use

    Bxbi site specific integration

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. StarShip03

    Plasmid

    #164507

    Purpose

    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES2-T7-Halo-PDGFR_TM-T2A-Puro.

    Depositor

    Bo Huang

    Type

    Empty backbone

    Use

    Bxbi site specific integration

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG-All-in-one-hCascade

    Plasmid

    #134919

    Purpose

    Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pInducer10-mir-RUP-PheS

    Plasmid

    #44011

    Purpose

    Lentiviral shRNA vector (pGIPz based) for inducible knockdown

    Depositor

    Stephen Elledge

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Insert

    PheS Gly294
    

    Use

    Lentiviral

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE2

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAD100-CHIP-myc-His

    Plasmid

    #236054

    Purpose

    Mammalian expression of human CHIP with a C-terminal myc-His tag

    Depositor

    Jonathan Schisler

    Article

    Stewart et al bioRxiv 2025.02.17.637997

    Insert

    CHIP (STUB1 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPV-Dual_promoter-EF1α-2xNLS-Cascade+Cas3-P (RD)

    Plasmid

    #204619

    Purpose

    Expression vector of Cascade and Cas3. Puromycin selectable.

    Depositor

    Akitsu Hotta

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cas7, Cas5, Cas8, Cas11, Cas6, and Cas3
    

    Use

    CRISPR

    Tags

    Each protein has C- and N-terminal SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pC-G418-YR

    Plasmid

    #61767

    Purpose

    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).

    Depositor

    Ken Haynes

    Article

    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

    Inserts

    nptII gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    

    Use

    Ternary vector for agrobacterium mediated transfo…

    Tags

    Expression

    Mutation

    Promoter

    trpC promoter

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHE111-3xFlag-HSP70

    Plasmid

    #236055

    Purpose

    Mammalian expression of human HSP70 with a N-terminal 3x-FLAG tag

    Depositor

    Jonathan Schisler

    Article

    Stewart et al bioRxiv 2025.02.17.637997

    Insert

    HSP70 (HSPA1A Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pETM6-G6-vioABE-4A6-vioC

    Plasmid

    #73439

    Purpose

    Deoxyviolacein pathway (vioABEC) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE) and 4A6 (vioC) for orthogonal flux redirection.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    PG6-vioABE-P4A6-vioC
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PG6 and P4A6 (orthogonal T7-lac variants)

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pICE-RNaseHI-D10R-E48R-NLS-mCherry

    Plasmid

    #60367

    Purpose

    Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Insert

    RNase HI-D10R-E48R
    

    Use

    Tags

    NLS from SV40 T antigen and mCherry

    Expression

    Mammalian

    Mutation

    D10R+E48R; catalyticaly inactive

    Promoter

    CMV-tet

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)

    Plasmid

    #169186

    Purpose

    To express His-SUMO-nsp7 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp7 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDRM18 LTN (Luciferase tdTomato Neo)

    Plasmid

    #174721

    Purpose

    Expresses the firefly luciferase gene, E2A, tdTomato, P2A, and the neomycin resistance gene.

    Depositor

    Wayne Tilley

    Article

    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

    Inserts

    Firefly Luciferase
    tdTomato
    aph
    

    Use

    Lentiviral and Luciferase

    Tags

    E2A linker and P2A linker

    Expression

    Mammalian

    Mutation

    Promoter

    MND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. lentiCRISPR-v2-HygR-EGFP

    Plasmid

    #167188

    Purpose

    Lentiviral expression of sgRNA with hygromycin resistance gene and EGFP

    Depositor

    Yin Shen

    Article

    Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

    Insert

    Hygromycin B phosphotransferase and EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-Etanercept

    Plasmid

    #182424

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-Etanercept fusion proteins

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-LZ-mNeongreen-Etanercept (TNFRSF1B Human)
    VNp-LZ
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp), and Vesicle Nuc…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)

    Plasmid

    #100597

    Purpose

    piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)

    Depositor

    Akitsu Hotta

    Article

    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

    Inserts

    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for human codon usage

    Promoter

    Dox-inducible TetO promoter and Human EEF1A1 prom…

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. SPACE (pRZ1813)

    Plasmid

    #140242

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V82G in TadA*, D10A in Cas9, R187W in pmCDA1

    Promoter

    CMV

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. SPACE-NG (pRZ5150)

    Plasmid

    #140244

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE_NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V82G in TadA*, NG mutations in SpCas9, D10A in Sp…

    Promoter

    CMV

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA

    Plasmid

    #134925

    Purpose

    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA

    Plasmid

    #134922

    Purpose

    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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46. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA

    Plasmid

    #134924

    Purpose

    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA

    Plasmid

    #134923

    Purpose

    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)

    Plasmid

    #100596

    Purpose

    piggyBac vector expressing Dual-regulated Cas9 (Puro resistance)

    Depositor

    Akitsu Hotta

    Article

    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

    Inserts

    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for human codon usage

    Promoter

    Dox-inducible TetO promoter and Human EEF1A1 prom…

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

    Plasmid

    #176534

    Purpose

    Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    CRISPR-associated IscB (Delaware Bay metagenome)
    

    Use

    Tags

    14xHis-MBP-bdSUMO and TwinStrep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. pRSFDuet-1_VNp-LZ_VNp-LZ-hGH

    Plasmid

    #182412

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine zipper-human growth hormone fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-LZ-hGH (GH1 Human)
    VNp-LZ
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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