Skip to main content
Addgene
Showing: 1941 - 1960 of 15153 results

  1. DHFR-GFP-th-NL1

    Plasmid
    #173007
    Purpose
    For light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    DHFR-GFP-NL1
    Use
    Tags
    DHFR and EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    173007-plasmid-map-sequence-id-339111

    Enlarge

  2. DHFR-GFP-th-GluA1

    Plasmid
    #173008
    Purpose
    For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    DHFR-GFP-th-GluA1
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    173008-plasmid-map-sequence-id-339527

    Enlarge

  3. pcDNA3-mCherry-TSC2(AA)

    Plasmid
    #192442
    Purpose
    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.
    Depositor
    Jin Zhang
    Article
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Insert
    mCherry-TSC2(S939A,T1439A) (TSC2 Human)
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …
    Promoter
    CMV
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    192442-plasmid-map-sequence-id-379128

    Enlarge

  5. P9_TEVs_cLuc

    Plasmid
    #119303
    Purpose
    Expresses C-terminus of split luciferase connected to P9 coil via TEVs cleavable linker/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split cLuc connected to P9 coil via TEVs cleavable linker
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    119303-plasmid-map-sequence-id-231805

    Enlarge

  10. pLV-REPORT(EF1a)-TTN

    Plasmid
    #172327
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Article
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    monomeric nuclear Tomato
    d2eGFP
    HygR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3x SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-alpha
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    172327-plasmid-map-sequence-id-354684

    Enlarge

  16. H2B-mScarlet- hRec8(297-506)-hMeikin(1-332, E151R, R154E)- mNeonGreen

    Plasmid
    #174716
    Purpose
    Stable expression of fluorescent Separase cleavage sensor with human Rec8 fused to human Meikin
    Depositor
    Iain Cheeseman
    Article
    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8
    Insert
    Rec8 (REC8 Human)
    Use
    Retroviral
    Tags
    H2B-mScarlet and mNeonGreen
    Expression
    Mammalian
    Mutation
    hRec8: 297-506; hMeikin: 1-332, E151R, R154E
    Promoter
    Available since
    Oct. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    174716-plasmid-map-sequence-id-343358

    Enlarge

Showing: 1941 - 1960 of 15153 results