We narrowed to 6,336 results for: WUS;

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Flag-ZDHHC11

Plasmid

#231659

Purpose

Expresses human ZDHHC11 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC11 (ZDHHC11 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-MtAcKRS (human-opti)

Plasmid

#164195

Purpose

To express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansion

Depositor

Tao Liu

Article

Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

Insert

MtAcKRS-478(human-opti)

Use

Synthetic Biology

Expression

Mammalian

Mutation

D76G; L325M; L329I; Y330F; L333A; C372F

Promoter

CMV

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42229

Purpose

This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCA14-pHR-SFFV-GFP-RPA43

Plasmid

#199445

Purpose

Expression of GFP-RPA43 in mammalian cells

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

RPA43 (POLR1F Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

SFFV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 human cofilin S3E

Plasmid

#50861

Purpose

expresses psuedo-phosporylated, non-activatable cofilin fused to EGFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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CMV-GEM-GECO1

Plasmid

#32442

Purpose

Mammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

GEM-GECO1

Expression

Mammalian

Mutation

GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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GFP-PASS

Plasmid

#193970

Purpose

Monitoring the production of phosphatidic acid with GFP-PASS (phosphatidic acid biosensor with superior sensitivity)

Depositor

Guangwei Du

Article

Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

Insert

PA biosenor

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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FSW-ALDH2-T2A-EGFP

Plasmid

#197036

Purpose

Express ALDH2 from hSyn1 promoter, co-expressed with EGFP

Depositor

Ronald Hart

Article

Sun et al Aging Cell. 2023 Feb;22(2):e13772. doi:

Insert

ALDH2 (ALDH2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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dTAG NLRP1

Plasmid

#166825

Purpose

dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Expression

Mammalian

Promoter

hPGK

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 human cofilin S3A

Plasmid

#50860

Purpose

expresses constituvely active cofilin fused to EGFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S3A constitutively active

Promoter

CMV

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-hsf-1-sgRNA-B

Plasmid

#177787

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hsf-1 (hsf-1 Nematode)

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a_NRL_P2A_Hygro_Barcode

Plasmid

#120469

Purpose

Barcoded lentiviral vector to express NRL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

NRL (NRL Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_MYC_P2A_Hygro_Barcode

Plasmid

#120461

Purpose

Barcoded lentiviral vector to express MYC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYC (MYC Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF17_P2A_Hygro_Barcode

Plasmid

#120503

Purpose

Barcoded lentiviral vector to express KLF17 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF17 (KLF17 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)

Plasmid

#101297

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Article

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Expression

Mammalian

Mutation

Contains amino acids 1-906 of alpha-Catenin

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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PL452-hGH-polyA-PGK-neoR

Plasmid

#191681

Purpose

Donor plasmid backbone PGK-neoR

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Type

Empty backbone

Expression

Mammalian

Mutation

WT

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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mEos3-Rac1

Plasmid

#92039

Purpose

Expresses mEos3-Rac1 fusion protein

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pDA077

Plasmid

#131388

Purpose

L1RP expression

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

L1RP

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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TJP1_PDZ_2

Plasmid

#103910

Purpose

Protein expression and purification of ZO1 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

TJP1 (TJP1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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TJP1_PDZ_1

Plasmid

#103909

Purpose

Protein expression and purification of ZO1 PDZ1 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

TJP1 (TJP1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.pU1a-SpCas9

Plasmid

#121507

Purpose

Expresses codon-optimized SpCas9 in mammalian cells.

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

SpCas9

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

U1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3A-BE3

Plasmid

#113410

Purpose

Expresses hA3A-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-BE3 (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CARD8-TEV-GFP-FLAG S297A

Plasmid

#169992

Purpose

Mammalian purification construct for CARD8 with an autoprocessing-deficient mutation

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

TEV-siriusGFP-FLAG

Expression

Mammalian

Mutation

S297A

Promoter

CMV

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-B1

Plasmid

#31705

Depositor

Duanqing Pei

Article

Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

Insert

miR-106a

Use

Retroviral

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pCIG-V5-Snail2-IRES-nls-GFP

Plasmid

#44282

Depositor

Martin Cheung

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

Insert

V5-Snail2 (SNAI2 Chicken)

Tags

V5

Expression

Mammalian

Promoter

CMV-IE-enhancer and chicken beta-actin promoter

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP hsp70 K71E

Plasmid

#15216

Depositor

Lois Greene

Article

Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

Insert

hsp70 K71E (HSPA1A Human)

Tags

EGFP

Expression

Mammalian

Mutation

K71E, ATPase-deficient mutant.

Available Since

June 15, 2007

Availability

Academic Institutions and Nonprofits only

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EF1a_LMX1A_P2A_Hygro_Barcode

Plasmid

#120457

Purpose

Barcoded lentiviral vector to express LMX1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

LMX1A (LMX1A Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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delCMV-mCherry-3x-p67phox-GBD

Plasmid

#214141

Purpose

Rac activity sensor

Depositor

Leif Dehmelt

Article

Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

Insert

3xp67phox-GBD (NCF2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Only the GTPase binding domain (GBD) is included

Promoter

delCMV

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSLIK-Rbfox2-rABE-Flag

Plasmid

#191384

Purpose

Doxycycline inducible expression of Rbfox2-rABE

Depositor

Stephen Floor

Article

Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

Insert

Rbfox2-rABE (RBFOX2 Human, Synthetic)

Use

Lentiviral

Tags

Flag

Promoter

tet-inducible promoter

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Trak1

Plasmid

#127621

Purpose

N-terminal GFP-tagged mouse Trak1

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Trak1 (Trak1 Mouse)

Tags

eGFP

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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