We narrowed to 24,628 results for: Nes

1. pHLuc Control

   Plasmid

   #141091

   Purpose

   pH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEP

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   Antares-T2A-puromycin-IRESv24-eGFP-Nanoluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 3xDBE_RE::Renilla

   Plasmid

   #124535

   Purpose

   Foxo Renilla luciferase reporter

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   FOXO Renilla reporter
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGB 35S:DsRed:Tnos (GB0361)

   Plasmid

   #68220

   Purpose

   Transcriptional unit for DsRed plant expression driven by the 35S promoter

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   DsRed
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   35S

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSNAP-CLC-EYFP

   Plasmid

   #38011

   Depositor

   Xiaowei Zhuang

   Article

   Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

   Insert

   Clathrin, light polypeptide (LCa) (Clta Mouse)
   

   Use

   Tags

   EYFP and SNAP26m

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pETM6-G6-vioABECD

   Plasmid

   #66536

   Purpose

   pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with G6 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Inserts

   VioA
   VioB
   VioE
   VioC
   VioD
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Mutant T7 Promoter - G6

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGrRenilla

   Plasmid

   #118060

   Purpose

   GBPart - GreenRenilla Luciferase CDS

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   Green Renilla
   

   Use

   Luciferase and Synthetic Biology; Gbpart

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRB14

   Plasmid

   #52522

   Purpose

   expresses a myc-tagged version of hCas9 in Drosophila

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   S. pyogenes cas9 with humanized codon bias
   

   Use

   Tags

   myc

   Expression

   Insect

   Mutation

   Promoter

   tubulin

   Available Since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-erHRP(N175S mutant)

   Plasmid

   #79909

   Purpose

   erHRP(N175S mutant) pCMV backbone

   Depositor

   Joshua Sanes

   Article

   Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

   Insert

   erHRP(N175S mutant)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   N175S mutation

   Promoter

   CMV

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pbGH

   Plasmid

   #118061

   Purpose

   GBPart - Bovine growth hormone terminator

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   bgH_poly(A)
   

   Use

   Synthetic Biology; Gbpart

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pVAX_CBI_AcrIIA4

    Plasmid

    #136662

    Purpose

    Expresses human codon-optimized AcrIIA4 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

    Depositor

    Yannick Doyon

    Article

    Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

    Insert

    AcrIIA4
    

    Use

    Synthetic Biology

    Tags

    No tag, No NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Orange Nano-lantern(Ca2+)/pcDNA3

    Plasmid

    #71346

    Purpose

    Express Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cell

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern(Ca2+)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pENTR221-NFAT5

    Plasmid

    #79506

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NFAT5 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    NFAT5 (NFAT5 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDragon-empty

    Plasmid

    #155018

    Purpose

    Empty Tigre-Dragon vector. Genes of interest can be cloned using Mlu-I and SnaB-I. For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA.

    Depositor

    Alberto Rosello-Diez

    Article

    Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EE-BE4max

    Plasmid

    #138158

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(EE)-nSpCas9-UGI-UGI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 R126E + R132E; within Cas9 D10A

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEYFP-Pds1(434)

    Plasmid

    #39847

    Depositor

    Jonathon Pines

    Article

    Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

    Insert

    Pds1 (PTTG1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJSK418

    Plasmid

    #205131

    Purpose

    Expression of GFP-2xFYVE in Dictyostelium

    Depositor

    Jason King

    Article

    Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

    Insert

    2xFYVE (from human Hrs) (HGS Synthetic)
    

    Use

    Dictyostelium expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a8

    Plasmid

    #124861

    Purpose

    Mutagenesis of Slc17a8

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a8 (Slc17a8 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KAP3-FL

    Plasmid

    #51413

    Purpose

    expression of EGFP-tagged full length human KAP3B in mammalian cells

    Depositor

    Trina Schroer

    Article

    Brown et al Traffic. 2005 Dec;6(12):1114-24.

    Insert

    Kinesin-associated protein 3 (KIFAP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pNLuc

    Plasmid

    #118058

    Purpose

    GBPart - NLuc Luciferase CDS

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    Nano Luciferase
    

    Use

    Luciferase and Synthetic Biology; Gbpart

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVAX_CBI_AcrIIA2

    Plasmid

    #136661

    Purpose

    Expresses human codon-optimized AcrIIA2 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

    Depositor

    Yannick Doyon

    Article

    Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

    Insert

    AcrIIA2
    

    Use

    Synthetic Biology

    Tags

    No tag, No NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHygromycin (GB0211)

    Plasmid

    #68204

    Purpose

    Provides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Hygromycin resistance gene coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGE-attBTT-loxP-DsRed (pTL780)

    Plasmid

    #115160

    Purpose

    Integration vector for phiC31-mediated transgenesis using a genomic standard attP-TT site. Contains an attB-TT site, the visible 3xP3-DsRed marker flanked by a loxP site for Cre-mediated removal.

    Depositor

    Tobias Langenhan

    Article

    Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

    Insert

    attB-TT loxP 3xP3-DsRed
    

    Use

    Cre/Lox

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. hCMV-IE1:GLuc:bgH

    Plasmid

    #135294

    Purpose

    Transcriptional unit for constitutive expression of GLuc in mammalian cells

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    CMV:GLuc:bGH
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLuciferase (GB0096)

    Plasmid

    #68201

    Purpose

    Provides the CDS of the firefly luciferase protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Luciferase coding region
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Orange Nano-lantern/pcDNA3

    Plasmid

    #67661

    Purpose

    orange luminescent protein for high-speed single-cell and whole body imaging

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

26. c3GIC9n

    Plasmid

    #62192

    Purpose

    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

    Depositor

    Lukas Dow

    Article

    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

    Insert

    humanized S. Pyogenes Cas9 (D10A)
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mutation

    D10A substitution (Cas9n)

    Promoter

    TRE3G

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Cyan Nano-lantern/pcDNA3

    Plasmid

    #67660

    Purpose

    cyan luminescent protein for high-speed single-cell and whole body imaging

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Cyan Nano-lantern
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSLIK 3xFLAG-TWIST2 neo

    Plasmid

    #197936

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-tagged TWIST2

    Depositor

    Kevin Janes

    Article

    Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

    Insert

    TWIST2 (TWIST2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mCerulean3-H2B-6

    Plasmid

    #55421

    Purpose

    Localization: Nucleus/Histones, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pJG85

    Plasmid

    #89281

    Purpose

    Entry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sites

    Depositor

    Daniel Voytas

    Article

    Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TB:7xSMAD_RE:MiniP

    Plasmid

    #133879

    Purpose

    7 copies the SMAD DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    7 copies the SMAD DNA binding motif
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLKO.1 shTGFBR3 puro

    Plasmid

    #58696

    Purpose

    Lentiviral shRNA vector for knockdown of human TGFBR3

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    shTGFBR3 (TGFBR3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

33. p5xE-Box

    Plasmid

    #118052

    Purpose

    GBPart - Operator/DNA Response element - 5 copies of the E-box motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    5 copies of the E-box motif
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pKMD106h - intein-Cas9(C574)

    Plasmid

    #64191

    Purpose

    Expresses intein-Cas9(C574) in mammalian cells

    Depositor

    David Liu

    Article

    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

    Insert

    mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG
    

    Use

    CRISPR

    Tags

    NLS - 3x FLAG (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

    Plasmid

    #87350

    Purpose

    S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/S355C/C574S/S867C

    Promoter

    T7

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pENTR221-SMAD3

    Plasmid

    #79507

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human SMAD3 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    SMAD3 (SMAD3 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    no Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TET-pLKO.1 PURO shSMAD4 #2

    Plasmid

    #83091

    Purpose

    Lentiviral shRNA vector for inducible knockdown of human SMAD4

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shSMAD4 (SMAD4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

38. pSLIK_hygro_3xflag_Luciferase

    Plasmid

    #136528

    Purpose

    Lentiviral expression vector for an inducible 3xflag-tagged Luciferase

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    Luciferase
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCfB3035(X-4 MarkerFree)

    Plasmid

    #73273

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. 35S::Cel1SP-GeNL/pCAMBIA1301

    Plasmid

    #190069

    Purpose

    Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplast

    Depositor

    Takeharu Nagai

    Article

    Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

    Insert

    Cel1 signal peptide-Green-enhanced Nano-lantern
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge