We narrowed to 10,757 results for: Kars

1. pCI-MS2V5-eRF3a F76a

   Plasmid

   #65811

   Purpose

   expression of eRF3a-MS2 fusion. F76A mutation abolishes binding to PABPC1

   Depositor

   Niels Gehring

   Article

   Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

   Insert

   eRF3a (GSPT1 Human)
   

   Use

   Tags

   MS2 stem loops and V5

   Expression

   Mammalian

   Mutation

   F76a

   Promoter

   CMV

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHAGE-FKBP-mEGFP-WIPI2d (R108E/R125E)

   Plasmid

   #223770

   Purpose

   Stable expression and inducible recruitment of protein in mammalian cell culture

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   WIPI2d (WIPI2 Human)
   

   Use

   Lentiviral

   Tags

   FKBP-mEGFP

   Expression

   Mutation

   R108E/R125E

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLX302_HOXB13

   Plasmid

   #70089

   Purpose

   Expression of human HOXB13 from CMV promoter, puromycin selection marker

   Depositor

   William Hahn

   Article

   Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

   Insert

   HOXB13 (HOXB13 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p413 TEF NAPstar3

   Plasmid

   #232740

   Purpose

   Expresses NADPH/NADP+ biosensor NAPstar3 in S. cerevisiae.

   Depositor

   Bruce Morgan

   Article

   Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

   Insert

   NAPstar3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EF1a_ESRRG_P2A_Hygro_Barcode

   Plasmid

   #120435

   Purpose

   Barcoded lentiviral vector to express ESRRG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   ESRRG (ESRRG Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EF1a_SOX2_P2A_Hygro_Barcode

   Plasmid

   #120480

   Purpose

   Barcoded lentiviral vector to express SOX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   SOX2 (SOX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEF1a-hMLH1dn (og codon)

   Plasmid

   #174823

   Purpose

   Mammalian expression of human MLH1dn (original codon usage)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   human MLH1dn (original codon usage) (MLH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deletion of residues 754-756

   Promoter

   EF1a

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Puro

   Plasmid

   #170317

   Purpose

   Expresses HA and EGFP tagged RPL22, puromycin resistance

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAR18_StrepI_mFASm_H8_pET22b

   Plasmid

   #122847

   Purpose

   Expresses murine type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

   Depositor

   Martin Grininger

   Article

   Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

   Insert

   mouse FASN/ mouse fatty acid synthase (Fasn Mouse)
   

   Use

   Tags

   His8 tag and Twin-Strep tag

   Expression

   Bacterial

   Mutation

   wildtype

   Promoter

   T7 promoter

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TetO-FUW-MEF2CA

    Plasmid

    #61538

    Purpose

    Expresses MEF2CA in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    MEF2CA (MEF2C Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pLV-EF1A-LivePAR(Y107A)-Hygro

    Plasmid

    #176073

    Purpose

    EGFP fused to the C-terminus of a WWE domain containing the mutation Tyr107Ala & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    LivePAR (Y107A)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Point mutation to convert Try107 to Ala

    Promoter

    EF1A

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJT306 Kp03 Lenti pEF-attB LSR GFP

    Plasmid

    #184949

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Patrick Hsu, Lacra Bintu, Ami Bhatt, Michael Bassik

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Kp03 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHAGE-ALDH5A1__MAP3K5

    Plasmid

    #116807

    Purpose

    Lentiviral expression of ALDH5A1__MAP3K5 fusion

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ALDH5A1__MAP3K5
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Puro

    Plasmid

    #170320

    Purpose

    Expresses HA and mCherry tagged RPL22, puromycin resistance

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCIneoGFP-BMI1

    Plasmid

    #128328

    Purpose

    Mammalian expression vector of GFP-tagged human BMI1

    Depositor

    Yutaka Hata

    Article

    Hossain et al Mol Cell Biol. 2018 Aug 15;38(17). pii:

    Insert

    BMI1 (BMI1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pD40-His/V5-c-MycT58A

    Plasmid

    #45598

    Depositor

    Rosalie Sears

    Article

    Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

    Insert

    c-Myc T58A (Myc Mouse)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    T58A

    Promoter

    CMV

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

17. Lenti-EFS-FLTID-GSQ-RBXN-P2A-BLAST

    Plasmid

    #208041

    Purpose

    Enables constitutive expression of TurboID alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; selection with blasticidin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    TurboID
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. BTG1

    Plasmid

    #155425

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    BTG1 (BTG1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EF1a_GATA4_P2A_Hygro_Barcode

    Plasmid

    #120444

    Purpose

    Barcoded lentiviral vector to express GATA4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    GATA4 (GATA4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Tet-O-FUW-EGFP-P2A-mCherry

    Plasmid

    #203868

    Purpose

    Tet-inducible lentiviral plasmid expressing EGFP-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Lenti-DIO-P-EF1a-RPL22-3xHA-IRES-YFP

    Plasmid

    #170327

    Purpose

    Double-floxed inverse Orientation HA and IRES-YFP tagged RPL22

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CPSF1

    Plasmid

    #155475

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CPSF1 (CPSF1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Neo

    Plasmid

    #170321

    Purpose

    Expresses HA and mCherry tagged RPL22, neomycin resistance

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEX Src-SH2

    Plasmid

    #46510

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Src (SRC Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 142-252

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EF1a_ETV2_P2A_Hygro_Barcode

    Plasmid

    #120436

    Purpose

    Barcoded lentiviral vector to express ETV2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    ETV2 (ETV2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBabe JUND-HA neo

    Plasmid

    #58489

    Purpose

    mammalian expression of human JUND

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    JUND (JUN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMSCV-LTR-dCas9-p65AD-BFP

    Plasmid

    #46913

    Purpose

    Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFP

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    dCas9-p65AD-BFP fusion
    Puromycin resistance
    

    Use

    CRISPR and Retroviral

    Tags

    2xNLS, BFP, and VP64 domain

    Expression

    Mammalian

    Mutation

    Promoter

    LTR and PGK

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CSTF1

    Plasmid

    #155485

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CSTF1 (CSTF1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. DDX24

    Plasmid

    #155512

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    DDX24 (DDX24 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. retro-puro vector

    Plasmid

    #58250

    Purpose

    Retroviral expression vector encoding an empty IRES-Puromycin cassette

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. EF1a_TFAP2C_P2A_Hygro_Barcode

    Plasmid

    #120487

    Purpose

    Barcoded lentiviral vector to express TFAP2C in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    TFAP2C (TFAP2C Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b

    Plasmid

    #122850

    Purpose

    Expresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coli

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    m(KS_MAT)_mouseL_ACP (Fasn Mouse)
    

    Use

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCIneoMyc UNC119Aa

    Plasmid

    #128331

    Purpose

    Mammalian expression vector of Myc-tagged human UNC119A isoform a

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

    Insert

    UNC119 (UNC119 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. EF1a_FOXA2_P2A_Hygro_Barcode

    Plasmid

    #120439

    Purpose

    Barcoded lentiviral vector to express FOXA2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    FOXA2 (FOXA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCIneoMyc UNC119Ab

    Plasmid

    #128332

    Purpose

    Mammalian expression vector of Myc-tagged human UNC119A isoform b

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

    Insert

    UNC119 (UNC119 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pPbcas13b

    Plasmid

    #89906

    Purpose

    Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    Cas13b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. EF1a_FOXA1_P2A_Hygro_Barcode

    Plasmid

    #120438

    Purpose

    Barcoded lentiviral vector to express FOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    FOXA1 (FOXA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. plenti-px330-CRBN-T1-pGK-Pur

    Plasmid

    #107382

    Purpose

    Mammalian expression CRISPR/Cas9

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    sgRNA targeting CRBN (CRBN Human)
    

    Use

    Lentiviral; Crispr

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1 EWS-myc-HIS

    Plasmid

    #46386

    Purpose

    expresses myc, HIS tagged EWSR1 in mammalian cells

    Depositor

    Heinz Gehring

    Article

    Pahlich et al Proteins. 2008 Sep;72(4):1125-37. doi: 1

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    His and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

40. FKBP-beta2(616-951)-GFP

    Plasmid

    #100726

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Long isoform

    Promoter

    CMV

    Available since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. ACE2-Fc

    Plasmid

    #164222

    Purpose

    Expresses soluble , dimeric Fc-His fusion protein for the human ACE2 extracellular domain, along with dTomato reporter

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

    Insert

    ACE2 extracellular domain (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pGLOW39

    Plasmid

    #173068

    Purpose

    mScarlet^SEC^3xMyc vector with ccdB sites for cloning homology arms

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    mScarlet-I-C1^SEC^3xMyc
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xMyc and C. elegans codon-optimized mScarlet

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. EF1a_ERG_P2A_Hygro_Barcode

    Plasmid

    #120434

    Purpose

    Barcoded lentiviral vector to express ERG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    ERG (ERG Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLV-CMV-XRCC1-EGFP-Hygro

    Plasmid

    #176062

    Purpose

    EGFP fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    X-ray repair cross complementing 1 (XRCC1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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45. EF1a_CDX2_P2A_Hygro_Barcode

    Plasmid

    #120432

    Purpose

    Barcoded lentiviral vector to express CDX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    CDX2 (CDX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. EF1a_HAND2_P2A_Hygro_Barcode

    Plasmid

    #120447

    Purpose

    Barcoded lentiviral vector to express HAND2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    HAND2 (HAND2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. EF1a_LMX1A_P2A_Hygro_Barcode

    Plasmid

    #120457

    Purpose

    Barcoded lentiviral vector to express LMX1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    LMX1A (LMX1A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pBK086

    Plasmid

    #183145

    Purpose

    pET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expression

    Depositor

    Daan Swarts

    Article

    Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

    Insert

    MapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. SFRS16

    Plasmid

    #156009

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SFRS16 (CLASRP Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pGK-HELLO-T2A-Cre LV

    Plasmid

    #202003

    Purpose

    Generates lentiviral vector for autochthonous KP-HELLO tumor initiation by intratracheal administration to KP mice

    Depositor

    Nikhil Joshi

    Article

    Cui et al Cell. 2021 Dec 9;184(25):6101-6118.e13.

    Insert

    HELLO-T2A-Cre
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    pGK

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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