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We narrowed to 68 results for: Toxoplasma gondii

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Showing: 1 - 20 of 68 results

  1. pET-15b(+) Toxoplasma gondii (delta prenylation motif)

    Plasmid
    #170789
    Purpose
    Expresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)
    Depositor
    Kalle Gehring
    Insert
    Toxoplasma gondii PRL
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lacI
    Available Since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    170789-plasmid-map-sequence-id-337771

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  3. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    3X FLAG and Nuclear localization signal
    Promoter
    TgTUB1 and Toxoplasma U6
    Available Since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52694-plasmid-map-sequence-id-82051

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  6. pTUB1:OsTIR1-3FLAG, SAG1:CAT

    Plasmid
    #87258
    Purpose
    TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.
    Depositor
    David Sibley
    Article
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    TIR1
    CAT
    Use
    Toxoplasma expression
    Tags
    3FLAG
    Mutation
    Codon optimized for Toxoplasma gondii expression
    Promoter
    TgSAG1 361 bp and TgTUB1 2717 bp
    Available Since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87258-plasmid-map-sequence-id-176556

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  8. pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT

    Plasmid
    #87259
    Purpose
    YFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133
    Depositor
    David Sibley
    Article
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    YFP-mAID-3HA
    HXGPRT
    Use
    Toxoplasma expression
    Tags
    3HA and mAID
    Mutation
    Codon optimized for Toxoplasma gondii expression
    Promoter
    TgDHFR-TS 463 bp and TgTUB1 378 bp
    Available Since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87259-plasmid-map-sequence-id-176555

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  9. pBM019

    Plasmid
    #128179
    Purpose
    Construct for generating stable Cas9-expressing Toxoplasma gondii
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003
    Inserts
    sgRNA#1
    SpCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Promoter
    TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…
    Available Since
    July 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    128179-plasmid-map-sequence-id-249095

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  11. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid
    #171089
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Mutation
    D10A, H840A
    Promoter
    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171089-plasmid-map-sequence-id-339962

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  12. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid
    #171090
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…
    Mutation
    D9A, H599A
    Promoter
    TUB1 (Toxoplasma gondii), transcriptionally linke…
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171090-plasmid-map-sequence-id-335786

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  15. pCvGAPDH-NanoLuc^opt

    Plasmid
    #255781
    Purpose
    Expresses NanoLuc luciferase (codon optimized for Chromera) in Chromera velia and Toxoplasma gondii
    Depositor
    Ke Hu
    Article
    Tengganu et al J Cell Sci. 2026 Jan 1;139(1):jcs.264400
    Insert
    Cv β-tubulin intron 1 + GAPDH Kozak + NanoLuc
    Use
    Chromera velia, toxoplasma gondii
    Expression
    Bacterial
    Mutation
    codon optimized for Chromera velia
    Promoter
    putative C. velia GAPDH promoter (1090 bp)
    Available Since
    June 10, 2026
    Availability
    Academic Institutions and Nonprofits only
    255781-plasmid-map-sequence-id-511276

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  16. pCvGAPDH-CvH2B-mStayGold

    Plasmid
    #255783
    Purpose
    Expresses mStayGold-tagged Chromera histone 2b protein (VEupathDB: Cvel_19200) in Chromera velia and Toxoplasma gondii
    Depositor
    Ke Hu
    Article
    Tengganu et al J Cell Sci. 2026 Jan 1;139(1):jcs.264400
    Insert
    Chromera velia histone H2B (VEupathDB: Cvel_19200)
    Use
    Chromera velia, toxoplasma gondii
    Tags
    mStayGold (codon-optimized for Chromera)
    Expression
    Bacterial
    Promoter
    putative C. velia GAPDH promoter (1090 bp)
    Available Since
    June 10, 2026
    Availability
    Academic Institutions and Nonprofits only
    255783-plasmid-map-sequence-id-511279

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  17. pCvGAPDH-CvACP-mStayGold

    Plasmid
    #255784
    Purpose
    Expresses mStayGold-tagged Chromera acyl carrier protein (VEupathDB: Cvel_32060) in Chromera velia and Toxoplasma gondii
    Depositor
    Ke Hu
    Article
    Tengganu et al J Cell Sci. 2026 Jan 1;139(1):jcs.264400
    Insert
    Chromera velia acyl carrier protein (VEupathDB: Cvel_32060)
    Use
    Chromera velia, toxoplasma gondii
    Tags
    PGTGSGGTGSGRS linker and mStayGold (codon-optimiz…
    Expression
    Bacterial
    Promoter
    putative C. velia GAPDH promoter (1090 bp)
    Available Since
    June 10, 2026
    Availability
    Academic Institutions and Nonprofits only
    255784-plasmid-map-sequence-id-511277

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  18. pCvGAPDH-CvIMC13-mStayGold

    Plasmid
    #255785
    Purpose
    Expresses mStayGold-tagged Chromera IMC13 protein (VEupathDB: Cvel_9514) in Chromera velia and Toxoplasma gondii
    Depositor
    Ke Hu
    Article
    Tengganu et al J Cell Sci. 2026 Jan 1;139(1):jcs.264400
    Insert
    Chromera velia inner membrane complex 13 protein (VEupathDB: Cvel_9514)
    Use
    Chromera velia, toxoplasma gondii
    Tags
    PGTGSGGTGSGRS linker and mStayGold (codon-optimiz…
    Expression
    Bacterial
    Promoter
    putative C. velia GAPDH promoter (1090 bp)
    Available Since
    June 10, 2026
    Availability
    Academic Institutions and Nonprofits only
    255785-plasmid-map-sequence-id-511278

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  19. pU6-Universal.Sth.dCas9

    Plasmid
    #171088
    Purpose
    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    Use
    Toxoplasma gondii expression
    Mutation
    Note: A S245F mutation is present but did not app…
    Promoter
    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171088-plasmid-map-sequence-id-335785

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Showing: 1 - 20 of 68 results