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1781_pAAV-U6-Ai9-Sa-gRNA1-CB-SACas9-HA-OLLAS-spA_C

Plasmid

#135979

Purpose

gRNA against the left loxP site of the Ai9 allele

Depositor

William Lagor

Insert

Ai9 gRNA1

Use

AAV, CRISPR, and Mouse Targeting

Tags

HA

Expression

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pX601-mCherry

Plasmid

#84039

Purpose

Staphylococcus aureus (SaCas9) conjugated with mCherry

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

Insert

SaCas9

Use

AAV and CRISPR

Tags

NLS and T2A-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pX601-GFP

Plasmid

#84040

Purpose

Staphylococcus aureus (SaCas9) conjugated with GFP

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

Insert

SaCas9

Use

AAV and CRISPR

Tags

NLS and T2A-EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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MSP2262

Plasmid

#70703

Purpose

Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Mutation

D10A & H557A in SaCas9

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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MSP2279

Plasmid

#70706

Purpose

Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Mutation

D10A and H557A in SaCas9

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pNS38-SadCas9-SNAP

Plasmid

#113718

Purpose

Bacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9

Depositor

Gerald Schwank

Article

Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

Insert

Cas9

Use

CRISPR

Tags

HA-2xNLS-SNAP-NLS and His6-MBP-TEV

Expression

Bacterial

Mutation

D10A, N580A

Promoter

Availability

Academic Institutions and Nonprofits only

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pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA

Plasmid

#61592

Purpose

A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Insert

hSaCas9

Use

AAV and CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pET28-SaCas9

Plasmid

#178901

Purpose

SaCas9 (Cas9 from Staphylococcus aureus)

Depositor

Hendrik Milting

Article

Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

Insert

SaCas9

Use

Tags

His6-Tag, bipartite nuclear localization signal f…

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA4_gb39_lenti

Plasmid

#138305

Purpose

Lenti expression of AcrIIA4

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA4

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF525-AcrIIA5_gb36_lenti

Plasmid

#138306

Purpose

Lenti expression of AcrIIA5

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA5

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF525-AcrIIA13b_gb76_GF31-2-hom_lenti

Plasmid

#138310

Purpose

Lenti expression of AcrIIA13b

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA13b

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF525-AcrIIA14_gb77_Cand9_lenti

Plasmid

#138311

Purpose

Lenti expression of AcrIIA14

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA14

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF525-AcrIIA15_gb79_Cand27_lenti

Plasmid

#138313

Purpose

Lenti expression of AcrIIA15

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA15

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF823_EFS-Cas9-PuroR_lenti

Plasmid

#138317

Purpose

Lenti expression of SpyCas9 with Puro selection

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

SpyCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLPhygSaCas9

Plasmid

#123262

Purpose

Expresses Staphylococcus aureus Cas9 (SaCas9) in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Insert

Staphylococcus aureus Cas9 (NEWENTRY )

Use

CRISPR; Leishmania

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0020

Plasmid

#117531

Purpose

Level0 Golden Gate part: CDS, SaCas9

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (with stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0021

Plasmid

#117532

Purpose

Level0 Golden Gate part: CDS, SaCas9 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPOR0SP0012

Plasmid

#117533

Purpose

Level0 Golden Gate part: CDS, eSaCas9 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

eSaCas9 (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

R499A,Q500K,R654A,G655R

Promoter

Availability

Academic Institutions and Nonprofits only

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pCAG-CFP-SaCas9-HF (without sgRNA)

Plasmid

#134470

Purpose

Human expression vector for SaCas9-HF variant.

Depositor

Zongli Zheng

Article

Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

Insert

Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity

Use

CRISPR

Tags

SV40NLS-3xFLAG

Expression

Mammalian

Mutation

R245A, N413A, N419A, R654A

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA14-truncated_gb85_Cand9-trunc_lenti

Plasmid

#138312

Purpose

Lenti expression of AcrIIA14, C-terminus

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA14, C terminus

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge