Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 849 results for: HA

Showing: 1 - 40 of 849 results

  1. Chemogenetics AAV Preps

    Type
    Collection
    ... pAAV SYN1 HA-hM4D(Gi) hM4D(Gi) - Inhibition HA fusion none 2, 5 Richie 121539 pAAV SYN1 HA-hM3D(Gq) hM3D... Roth 50454 pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine hM3D(Gq) - Activation HA fusion and IRES mCitrine ... Roth 50455 pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine ... hM3D(Gq) - Activation HA fusion none 2, 5 Richie 50467 pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi...* Fishell 50472 pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine rM3D(Gs) - Activation HA fusion and IRES mCitrine ...CAG E2 regulatory element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity Cre-dependent...1, 2, 5, 8, 9, rg*, PHPeB Roth 65417 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine kappa-opioid receptor (KOR) - ...

  2. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pCINeo-hFX-HA FXN HA, AU1 CMV Friedreich ataxia Michael Ristow 14977 pCINeo-hFX(I154F)-HA FXN HA, AU1 CMV...pBlue-hFX-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14988 pBlue-hFX(I154F)-HA FXN HA, AU1 T7 Friedreich...pBlue-hFX(T133A)-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14990 pBlue-hFX(T133E)-HA FXN HA, AU1 T7 Friedreich...-hFX-HA FXN HA, HIS, AU1 T7 Friedreich ataxia Michael Ristow 14992 pET28(a)-hFX(I154F)-HA FXN HA, HIS,...AA88)-HA FXN HA, HIS, AU1 T7 Friedreich ataxia Michael Ristow 15403 pBlue-hFX(I154F)(AA88)-HA FXN HA, HIS...pBlue-hFX-HA(AscI) FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 15405 pBlue-hFX-HA(NcoI/EcoRI) FXN HA, AU1...Camilli 22563 Flag-HA-UCHL1 UCHL1 HA, FLAG Parkinson's Wade Harper 22594 Flag-HA-OTUD4 OTUD4 HA, FLAG CMV Gordon...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...tag c-Flag pcDNA3 or FLAG-HA-pcDNA3.1 - C-terminal Flag or N-terminal FLAG-HA for mammalian expression ...systems, including Xenopus HA Epitope tag pcDNA3.1-HA or c-Flag pcDNA3 - Mammalian expression...expression vector with N- or C-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF...gene with cap-dependent 3Myc-EYFP-HA-His6 and IRES-dependent ECFP-HA-His6 translation for mammalian expression... your protein to an epitope tag, such as Flag or HA, will allow you to easily identify your protein...tag pE2c - Modified pENTR vector for C-terminal 3x HA tag fusion with your gene of interest in plants Myc...but do NOT integrate into the host genome Addgene has an extensive collection of plasmids for packaging...

  4. Distribution to Industry

    Type
    Collection
    ...Addgene has a growing collection of plasmids that are available to industry. Learn more about the available... it with your organization. If your organization has not ordered from Addgene in the past, we will need...regarding your account verification. Once your account has been verified, you will be able to place your order...our Help Center . The MTA Process: Once your order has been placed on our website, Addgene will email you...behalf of the recipient institution. Once the MTA has been signed, it can be submitted to Addgene through...

  5. TALEN Guide

    Type
    Collection
    ...fairly high specificity. Fifteen years of research has shown that when fused to a nuclease or activation...limitations, however. Not every nucleotide triplet has a corresponding zinc finger, and interactions between...with Adam Bogdanove ), progress on TALe research has been swift. The incentives are high. Since ZF targets... TALEN kit came online in the summer of 2011 and has already become the best-selling kit in Addgene’s ...co-founder of the Zinc Finger Consortium with Dr. Voytas, has considerable experience in the DNA targeting field...become a key tool in your plasmid toolbox. Addgene has rapidly become an important resource for this new...

  6. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...wildtype Cas9 with optimized chimeric gRNA has high efficiency but has been shown to have off-target effects...seminal CRISPR article from Dr. Feng Zhang's Lab, has assembled a list of FAQs about using the lab's CRISPR...Dr. Feng Zhang's Lab ( Science , February 2013 ), has assembled a list of FAQs about using the lab's CRISPR... a new system, developed by the Zhang lab, which has comparable efficiency to the optimized chimeric design...of references. For genome editing, the Zhang lab has published this Nature Protocol article . Should I...sequence is present in both alleles. The Zhang lab has found that if a CRISPR is used to engineer a locus...relative to where the homology ends? The Zhang lab has always used homology arms that are less than 15bp...

  7. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Design Tools CRISPR Blog Posts The CRISPR system has been adapted to allow tagging of proteins expressed...Yamamoto PITCh Tagging System The Takashi Yamamoto lab has deposited the PITCh system plasmids for CRISPR-based...Auxin-Inducible Degron Tagging Masato Kanemaki's lab has developed a simple and scalable CRISPR/Cas-based ...Drosophila Cell Tagging System The Förstemann lab has developed a CRISPR tagging technique for use in Drosophila...et al. Nucleic Acids Res. 2014 The Förstemann lab has provided detailed protocols for N- or C-terminal .... elegans Tagging System Geraldine Seydoux's lab has developed a systematic and scalable method to create... Collection The Allen Institute for Cell Science has produced the first publicly available collection ...

  8. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ... visualize targeted genomic loci. CRISPR imaging has numerous advantages over other imaging techniques...dynamics in living cells. The fluorescent CRISPR system has been used for dynamic tracking of repetitive and ...visualized in living cells using a single gRNA that has multiple targeted sequences in close proximity. A...

  9. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...sequence has been validated by next generation sequencing. The DNA concentration and 260/280 ratio has been...See What You’re Looking For? At this time, Addgene has made a limited number of plasmids available as cloning...

  10. Tetracycline Inducible Expression

    Type
    Collection
    ...in both tetracycline on and off systems. Dox also has good tissue distribution, low toxicity, a known half-life...expression of shRNA; neomycin selection; plasmid 21915 has puromycin selection TetR On Wiederschain 41393 pCW57.1...that is silent in absence of binding tTA or rTA; has high copy E. coli origin None Either Ralser 64238...60495 pSBtet-GP Sleeping Beauty transposon system; has luciferase in cloning site; see article for additional...to make cell lines Tet-On Advanced; Plasmid 26730 has hygro resistance 3rd generation rtTA On Campeau 25434...linked via IRES to EGFP-Blasticidin fusion; pMA2641 has rtTA driven by retroviral LTR promoters ; see article...

  11. COVID-19 Resources

    Type
    Collection
    ...plasmids. Ginkgo Bioworks Plasmid Collection - Addgene has partnered with Ginkgo Bioworks to distribute SARS-CoV...DETECTR . Feng Zhang's lab at the Broad Institute has utilized the natural RNAse activity of the Cas13 ...UnLOCKING (SHERLOCK and SHERLOCKv2). The Broad Institute has published information and protocols for utilizing...bioRxiv 2020.04.20.052159 . Jennifer Doudna's lab has utilized Cas12a’s non-specific ssDNA degradation ...CRISPR Trans Reporter (DETECTR). Mammoth Biosciences has published information and protocols for utilizing... out this Suggest a Plasmid form. If the plasmid has been described previously we will reach out to the...

  12. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...of Michael Davidson from Florida State University has contributed their comprehensive collection of ORFs... data found below. In addition, the Davidson lab has contributed the corresponding empty backbone with...

  13. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ... proteins in several expression systems. Addgene has partnered with Ginkgo to make these plasmids available...yeast, or mammalian) Tags (e.g. 6xHis, Flag, StrepII, HA, Myc, SUMO, GST, MBP, CBP, S-tag, TAP tag, TRX, or...

  14. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...The Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins...Plasmids Protocols The Allen Institute for Cell Science has produced the first publicly available collection .... Protocols The Allen Institute for Cell Science has several protocol videos available that may be helpful...

  15. TALEN Engineering

    Type
    Collection
    ...Voytas Lab plasmids CRISPR plasmids The Joung Lab has developed three platforms for engineering TAL effector...effector repeat arrays produced on this framework has yielded TALENs which function efficiently in: C. ...

  16. Luciferase Plasmid Collection

    Type
    Collection
    ... dynamic range, and resistance to photobleaching has made luciferase a common choice in assays ranging...second-generation version of Firefly luciferase that has been codon optimized for expression in mammalian ... Firefly luciferase, Click-beetle red luciferase has also been codon optimized ( CBR2opt ) for mammalian...mammalian expression systems. Each type of luciferase has advantages and disadvantages depending on the application...before or after luciferase . In addition, Addgene has many luciferase expression constructs available that...expression renilla luciferase mutant Rluc8, which has increased stability and light output Sanjiv Sam Gambhir...

  17. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ... for Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs that function in zebrafish... The Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs that function in zebrafish...

  18. Zebrafish Plasmid Collection

    Type
    Collection
    ...tropical freshwater fish in the minnow family. It has long been established as a powerful vertebrate model...genes having an ortholog in zebrafish, this species has more recently emerged as a model system for human... amino- or carboxyl-terminal tags, including Myc, HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible...

  19. TALEN Plasmids and Kits

    Type
    Collection
    ...respectfully). This next generation architecture has been shown to increase mutation induction when using...after the repeat domain, and C-terminal SV40 NLS and HA tags. Plasmid 47389 also contains the VP64 domain...double-selection of transfected cells, the system has successfully been employed for deletion mutagenesis...

  20. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...New England Biolabs has deposited plasmids at Addgene to generate SNAP, CLIP, ACP or MCP tagged plasmids...PDF, 46 KB) Please note: New England Biolabs (NEB) has discontinued the ACP-tag/MCP-tag product line from...

  21. CRISPR Guide

    Type
    Collection
    .... Cas9 nickase (Cas9n), a D10A mutant of SpCas9, has one active nuclease domain and one inactivated one... available. SaCas9 (from Staphylococcus aureus ) has a coding sequence ~1 kb shorter than SpCas9 while... SpCas9. FrCas9 (from Faecalibaculum rodentium ) has increased fidelity, and recognizes NNTA, a PAM sequence... create the adenine base editor. The TadA domain has also been further evolved to catalyze cytidine deamination...editing requires more optimization and typically has lower efficiency (< 50%) than base editing (> 90%...modifications to eliminate target gene function. It has also been used extensively to screen for novel genes... those for yeast or drosophila . Although CRISPR has been less widely used in bacteria due to technical...

  22. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Technological Advances CRISPR technology development has occurred at a truly astonishing pace, with work directed...Directions It’s amazing to see how much progress has been made in just five years, with the skyrocketing...see results! Getting Started with CRISPR Addgene has empowered researchers to further develop the CRISPR... find CRISPR plasmids for your research. Addgene has CRISPR plasmids for the following models: Mammalian...

  24. Antibody Guide

    Type
    Collection
    ... immune response to it. Once the immune response has developed, the antibodies are collected, tested against...epitopes on constant regions of other antibodies. This has useful research applications, and is covered in the...exist as monomers. Each antibody class therefore has a valency, or number of binding sites available per...of antibodies multiplied by two, as each antibody has two binding sites. Isotype Description Configuration... must be able to detect if and where an antibody has bound. Signaling molecules , proteins that emit a...to avoid confusing data. Check that your machine has appropriate lasers and filters for each fluorophore...

  25. Viral Vectors

    Type
    Collection
    ...plasmid DNA for use with virus-mediated delivery has many advantages for research. Viruses facilitate ...Kb 80-130 Dividing Yes Moderate * Recombinant AAV has a low frequency of target cell genome integration...

  26. Viral Production

    Type
    Collection
    ...below. AAV Production AAV distributed by Addgene has been produced either in-house by Addgene scientists...tested for mycoplasma contamination. To date, Addgene has never had a case of mycoplasma contamination. In ...

  28. MAPK Plasmids

    Type
    Collection
    ...numerous research labs from around the globe, Addgene has assembled a MAPK plasmid collection for community...

  30. Deisseroth INTRSECT Collection

    Type
    Collection
    ...these tools in well-defined cellular sub-populations has generally been limited to single-component cellular...Flp, Cre NOT Flp, or Flp NOT Cre. B) This strategy has been expanded to incorporate VCre in order to enable...using EYFP and ChR2-EYFP as payloads). This approach has been broadly applied using multiple recombinase-expression...

  33. CRISPR Plasmids - Parasites

    Type
    Collection
    ...parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for some of these parasites...

  34. AAV Viral Preps

    Type
    Collection
    ...range of experiments, both in vitro and in vivo. It has low immunogenicity, is easy to use, and can be safely...

  35. Institute for Protein Innovation

    Type
    Collection
    ...Recommended Applications Browse Epitope Tag Antibodies IPI has developed recombinant versions of antibodies that...

  36. Plasmid Collections

    Type
    Collection
    ...Vectors Communities Find a scientific community that has established a common place for deposited plasmids...

  38. Neurodegeneration Research Collection

    Type
    Collection
    ... Neurodegeneration Research Collection Addgene has assembled the following to help you find information...Parkinson's today. The Michael J. Fox Foundation has made a number of tools (Link opens in a new window...

  39. Bacterial Expression Systems

    Type
    Collection
    ... your gene of interest once its appropriate cDNA has been fused to the MS2 binding sites within this plasmid...expression only at a specific time. This collection has a variety of plasmids whose expression levels have...

  40. Zhang Lab CRISPR Page

    Type
    Collection
    ...to top AAV - In vivo Genome Editing The Zhang Lab has both single- and dual-vector systems that allow the...uses a smaller Cas9 from S. aureus (SaCas9) that has been human codon-optimized, and packages both the... : PX552; U6 promoter-driven; for sgRNA cloning; has GFP-KASH for FACS sorting SaCas9 + single guide RNA...
Showing: 1 - 40 of 849 results