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We narrowed to 1,100 results for: Ide

Showing: 1 - 40 of 1100 results

  1. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Xenopus. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Publication Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert...Publication C. elegans ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...Publication Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Xenopus ID Plasmid Gene/...Promoter Selectable Marker PI Publication Parasites ID Plasmid Gene/Insert Promoter Selectable Marker PI...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...efficiency than NHEJ. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...

  2. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI...diverse populations of mutants for directed evolution. ID Plasmid Gene/Insert Promoter Selectable Marker PI...specific edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker Publication...binding site and the desired edits on an RT template. ID Plasmid Gene/Insert Promoter Selectable Marker PI...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI...target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI... and are potentially heritable in dividing cells. ID Plasmid Gene/Insert Selectable Marker PI Publication...

  3. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter...Publication C. elegans ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...your specific locus. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna...

  4. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse... A->G (or T->C on the opposite strand) mutations. ID Plasmid Promoter PI Publication Nick CRISPR/Cas nickase...specific edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime...binding site and the desired edits on an RT template. ID Plasmid Gene/Insert Promoter PI Publication Activate...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Interfere...target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication RNA Targeting...

  5. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Publication Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Description Gene/Insert Promoter Selectable...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...homology-directed repair (HDR). Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna...

  6. Serotype Testing AAV

    Type
    Collection
    ...material page (linked for each catalog ID below). Serotype Catalog ID Volume Price* AAV1 37825-AAV1.T 20 ...material page (linked for each catalog ID below). Serotype Catalog ID Volume Price* AAV1 50465-AAV1.T 20 ...please see our Control AAV Inventory . The catalog ID for the 20 µL sized testing sample is denoted with...with a .T suffix (for example, AAV1.T). The catalog ID for the 100 µL sized sample does not have the suffix...

  7. CRISPR Plasmids - Yeast

    Type
    Collection
    ...protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI... A->G (or T->C on the opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI...specific edits via homology-directed repair (HDR). ID Plasmid Description Gene/Insert Promoter Selectable...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI...target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI...transcription factor and other protein binding sites. ID Plasmid Gene/Insert Promoter Selectable Marker PI...

  8. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog... or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering...your specific locus. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna...

  9. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...zebrafish. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...Promoter Selectable Marker PI Publication Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI...systems create a wide spectrum of single-nucleotide variants (somatic hypermutation) in a wider editing window...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have been made by...cytidine deaminase (AID). Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...

  10. CRISPR Plasmids - Plants

    Type
    Collection
    ...protein can be paired with a wide variety of Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication... A->G (or T->C on the opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI...specific edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker PI...binding site and the desired edits on an RT template. ID Plasmid Gene/Insert Promoter Selectable Marker PI...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI...target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI...

  11. Recombinases AAV Preps

    Type
    Collection
    ... information about these molecular tools. Cre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 105551...107787 AAV.TBG.PI.Cre.rBGe TBG none 8 Wilson Dre AAV ID Name Promoter Fluorophore Serotype(s) PI 50363 AAV...phSyn1(S)-DreO-bGHpA Syn none 5, rg* Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV... none 1, rg* Janelia Light-Inducible Recombinases ID Name Promoter Fluorophore Serotype(s) PI 140135 pAAV-EF1a-iCreV... pAAV-EF1a-iFlpV EF1a none 1, PHPeB Zeng VCre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 55638...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of...

  12. Institute for Protein Innovation

    Type
    Collection
    ...toolset for scientists worldwide. Figure courtesy of IPI Browse Glypican Antibodies ID Recombinant Antibody...Figure courtesy of IPI Browse Integrin Antibodies ID Recombinant Antibody Description Isotype Reactivity... epitope tags and other common cellular proteins. ID Recombinant Antibody Description Isotype Reactivity...used to create or express plasmid-based antibodies. ID Plasmid Description...the alpha subunit, outside the ligand-binding pocket Bind to the beta subunit, outside the ligand-binding...ligand-binding pocket Bind specific subunit pairs, outside the ligand-binding pocket Bind specific subunit pairs,...

  13. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Nick ...specific edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime...binding site and the desired edits on an RT template. ID Plasmid Gene/Insert Promoter PI Publication Activate...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog... base-to-base conversions using a prime editing guide RNA (pegRNA). The pegRNA directs the nickase to ...

  14. AAV Molecular Tools

    Type
    Collection
    ...tetracycline (tet)-inducible expression systems . ID Name Expression System Activity Serotype PI 99118... purification (which can be used for viral TRAP). ID Name Expression System Activity Serotype PI 98747...experiments, including neuronal connectivity labeling. ID Name Expression System Activity Serotype PI 51509... encode tools that can be used for cell ablation. ID Name Expression System Activity Serotype PI 124364...

  15. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Drosophila. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert ...Insert Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...

  16. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Drosophila. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene...whether the plasmid contains Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...deletions, and point mutations using a prime editing guide RNA (pegRNA). As with a typical gRNA, the pegRNA...

  17. Open Enzyme Collection

    Type
    Collection
    ...RNA Polymerases Other Enzymes DNA Ligases Addgene ID Plasmid Gene/insert 165503 pOpen-HSlig4 DNA Ligase... Ligase III Return to top DNA Polymerases Addgene ID Plasmid Gene/insert 165500 pOpen-9N7polA 9 degrees...Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid Gene/insert 165504 pOpen-EcoRIR EcoRI 165506...NcoI Return to top Reverse Transcriptases Addgene ID Plasmid Gene/insert 165546 pOpen-MMLV_RT (mut H) ...Transcriptase Return to top RNA Polymerases Addgene ID Plasmid Gene/insert 165505 pOpen-T7gene1 T7 RNA Polymerase...RNA Polymerase Return to top Other Enzymes Addgene ID Plasmid Gene/insert 165509 pOpen-TEV protease TEV...consists of more than 75 genes for enzymes that are considered "workhorses" of molecular biology, including ...

  18. Antibody Guide

    Type
    Collection
    ... Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection...This method uses peptide identification by proteomics (Capture MS) to validate the identity of proteins recognized...first identify your assay type and what species your protein of interest comes from. Then consider the ...In our Antibody Guide, we define key terms, summarize antibody production and storage techniques, and...Collection Jump To: Antibody Guide Production Structure Isotypes Antigen Specificity Other Affinity Reagents...Antibodies can be used in the lab to quantify, identify, separate, and locate proteins of interest. The... between antibodies, allows for the antibody’s identity to be easily confirmed via sequencing, and enables...

  19. CRISPR Guide

    Type
    Collection
    ...Science Guides CRISPR Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large...making CRISPR perfect for genome-wide screens. This guide will provide a basic understanding of CRISPR ...genomic target of the gRNA can be any ~20 nucleotide sequence, provided it meets two conditions: The sequence...discuss more Cas9 fusion tools later in the guide. Increasing fidelity of Cas9 Researchers have also enhanced...Cas9 into high fidelity Cas9s (hfCas9) . There is currently no defined measure of fidelity, so sometimes...with higher fidelity than SpCas9. FrCas9 (from Faecalibaculum rodentium ) has increased fidelity, and recognizes...some base editing systems create a wide spectrum of single-nucleotide variants (somatic hypermutation) ...

  20. TALEN Guide

    Type
    Collection
    ... TALENs TALEN Guide Addgene's Guide to TALEN Technologies...Scientists have identified a large number of zinc fingers that recognize various nucleotide triplets. With...Guide to TALEN technology and using TALEN plasmids TALENs...disciplines are coming closer to this DNA targeting ideal. Figure 1: (A) Representation of a zinc finger (...components of the array recognize individual nucleotides. For over ten years, zinc finger (ZF) arrays ...without their limitations, however. Not every nucleotide triplet has a corresponding zinc finger, and ...Bogdanove at Iowa State University published the nucleotide recognition code of the TAL effectors, which ...

  21. Lentiviral Prep Service

    Type
    Collection
    .... For more information, see our Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection...DSBs or cuts ) or single-stranded breaks ( nicks ). ID Name Insert Function Antibiotic Resistance Activity...transactivator and used as a transcriptional activator . ID Name Insert Antibiotic Resistance Activity PI 61422...VP64-2A-Blast-WPRE) Zhang Pooled Barcoding Libraries ID Name Description PI 115643 Morris CellTag Barcode...our repository that express fluorescent proteins. ID Name Insert Antibiotic Resistance Purpose PI 17446...sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting 19,114...sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637...

  22. Deisseroth INTRSECT Collection

    Type
    Collection
    ... and rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ...pAAV-CaMKIIa-Flpo None No Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ... Dual recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ... Cre Yes Dual recombinase-dependent: GECI Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ... recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ... recombinase-dependent: Inhibitory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ...Cre Yes Dual recombinase-dependent: DREADDs Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ...

  23. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying molecules associated... are more straightforward and enable direct identification of molecules associated with a genomic region...

  24. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    .... Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID Plasmid Gene...Gene/Insert PI Publication Plant ID Plasmid Gene/Insert PI Publication Do you have suggestions for other...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...regardless of homology to the crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require...require that each DNA target have high sequence identity to the spacer sequence and contain a PAM sequence...degradation seen in bacteria. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna...

  25. Fluorescent Protein Guide

    Type
    Collection
    ...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ... Special Collections Fluorescent Protein Guide Fluorescent Protein Plasmids & Resources You May... For Multi-Color Imaging Blog: Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Fluorescent...variety of fluorescent protein plasmids. Use this guide to learn more about the many applications of fluorescent...fluorescent biosensors. Optogenetics Plasmids | Guide Use light to detect, measure, and control molecular... actuators or sensors. Chemogenetics Plasmids | Guide Use small molecules to activate genetically engineered...Localization Determine where your protein of interest resides by using a well-characterized fluorescent fusion...

  26. Caltech Systemic Capsids

    Type
    Collection
    ...opens in a new window) . Browse Available PHP.eB AAV ID Name Promoter Description Category PI Controls 28306...plasmid (Addgene #103006) . Browse Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-...plasmid (Addgene #127847) . Browse Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP... (Addgene #185136) . Browse Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-... (Addgene #185137) . Browse Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-... (Addgene #175004) . Browse Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-... (Addgene #175005) . Browse Available CAP-B22 AAV ID Name Promoter Description Category PI 37825 pAAV-...

  27. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...and plants. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Bacteria ID Plasmid Gene/Insert Promoter... Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter PI Publication Last reviewed on: January...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...

  28. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...mTORC1 Activity in Living Cells", "mitochondria"). ID Plasmid Description Gene/Insert Vector Type PI Publication...Arosio Chloride (Cl-) Dual ClopHensorN chloride and proton sensor A genetically-encoded chloride and pH...1295-7. Samuel Miller Chloride (Cl-) ClopHensor biosensor for intracellular chloride and pH Arosio Lab ClopHensor...(6):e99095. Joseph Santos-Sacchi Chloride (Cl-) ClopHensorN chloride sensor (cytosolic) Multiplexed Optical...Cohen Chloride (Cl-) Membrane-targeted biosensor for monitoring changes in intracellular chloride A sensitive...Okumoto Hydrogen Peroxide (H 2 O 2 ) Fluorescent reporter of cytosolic hydrogen peroxide Monitoring the ...Hydrogen Peroxide (H 2 O 2 ) HyPer biosensor to measure changes in intracellular hydrogen peroxide concentration...

  29. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...glycine- and serine-containing peptide linker (GGSGGS) repeat pET28CLY2 Peptide linker standard consisting...Collections Fluorescent Proteins FRET Fluorescent Protein Guide: FRET More Fluorescent Protein Resources: Empty ...proteins connected via 5 amino acid linkers pET28CLY1 Peptide linker standard consisting of ECFP and EYFP connected...and EYFP connected by 2 GGSGGS repeats pET28CLY3 Peptide linker standard consisting of ECFP and EYFP connected...connected by 3 GGSGGS repeats pET28CLY4 Peptide linker standard consisting of ECFP and EYFP connected by... by 4 GGSGGS repeats pET28CLY5 Peptide linker standard consisting of ECFP and EYFP connected by 5 GGSGGS...GGSGGS repeats pET28CLY6 Peptide linker standard consisting of ECFP and EYFP connected by 6 GGSGGS repeats...

  30. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...cis -plasmid or transfer plasmid). Helper Plasmid ID Plasmid Serotype Description PI 112867 pAdDeltaF6...Available as a Viral Vector Packaging Service at Addgene ID Plasmid Capsid Description PI 112862 pAAV2/1 AAV1...this table based on plasmid name, capsid, and more. ID Plasmid Gene/Insert Mutations PI AAV Plasmids This...based on plasmid name, species, gene name, and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations...AAV) Plasmids Additional Resources: AAV Guide Biosafety Guide All Viral Plasmids Blog: Plasmids 101-Viral... virus. Instead, specific gene products can be provided by helper plasmids (e.g., pHelper) and specific...HEK293 cells) during AAV production. Read our AAV Guide for more information about: AAV components Common...

  32. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...Collections Fluorescent Proteins In Vivo Imaging Guide Fluorescent Proteins: In Vivo Imaging More Fluorescent...photoactivatable fluorescent empty backbone plasmids are ideal for single or multicolor in vivo imaging. Plasmids...

  33. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...markers of general cellular organelles and structures. ID Plasmid Structure Targeting Gene/Signal Fluorescent...table below to find markers of cytoskeletal element. ID Plasmid Structure Targeting Gene/Signal Fluorescent...table below to find markers for cellular junctions. ID Plasmid Structure Targeting Gene/Signal Fluorescent... to find markers of secretory pathway components. ID Plasmid Structure Targeting Gene/Signal Fluorescent...encoding fluorescent proteins tagged with genes or peptides with known subcellular localization patterns. ...important to determine where your protein of interest resides. There are a variety of methods for determining...red fluorescent protein (see this fusion protein guide to find the appropriate backbone). Then, by examining...

  34. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Collections Fluorescent Protein Guide Empty Backbones Fluorescent Protein Guide: Empty Backbones More Fluorescent... For Multi-Color Imaging Blog: Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Blog...product of exctinction coefficient and quantum yield divided by 1000. pKa is the pH at which fluorescence intensity...Yeast Thorn Lab Vectors - Includes tagging with a wide variety of colors, some specifically optimized for...

  35. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ... Synthetic Biology SynBio Guide Synthetic Biology: Assembly Standards Guide SynBio Resources SynBio Home...after the promoter, and so on. The following table provides information for the assembly standards most commonly...standards, there are additional sites which should be avoided whenever possible. Other popular cloning strategies...MoClo . Check out our Molecular Cloning Techniques Guide for an overview of the most common methods. BioBrick...

  36. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Activate...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...

  37. Michael J Fox Foundation (MJFF) Plasmid Collection

    Type
    Collection
    ...proteins of interest. Browse or search the table below. ID Plasmid Gene/Insert Mutations Industry Return to ...deposited with Addgene. Browse or search the table below. ID Plasmid Description Gene/Insert Industry PI Return...

  38. Adenovirus Plasmids

    Type
    Collection
    ...Strains Bacterial strains for generating adenovirus. ID Strain Description PI 16398 BJ5183 Strain for recombination...for adenoviral transfer plasmids that have inserts. ID Plasmid Type Description PI 16400 pAdEasy-1 Adenoviral...genome that can be assembled in a one-tube reaction. ID Kit Description PI 1000000176 AdenoBuilder toolkit...Adenovirus Plasmids Additional Resources: Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids...generate adenovirus particles. Read our Adenoviral Guide for more information about: Adenoviral components...

  39. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...systems. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...local chromatin state. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna...

  40. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ... 24 bp sequence, to simplify identification during screening. Library ID PI Description MORF library 137000...out PCR for 10X scRNA-seq (PDF, 59.2 KB) Plasmids ID Plasmid Gene/Insert Pooled Library The MORF pooled... has a unique 24 bp barcode that simplifies identification of the transcription factor isoform in pooled...
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