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  1. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...plants. Further opportunities with pSiM24 While these properties of pSiM24 are suitable for many applications... of DNA that will be transferred to the plant, similar to viral vectors’ terminal repeats. It’s a lot ...larger cloning capacity would be very useful: enter pSIM24! To build a better binary vector, Sahoo et al. ... Sahoo et al. demonstrate that this new vector, pSiM24, can also be used with biolistic methods, making...Agrobacterium infection. At approximately 7 kb, pSiM24 is 2 and 6 kb smaller than commercially available...blotting. Given its versatility and customizability, pSiM24 can be used not only for plant engineering and ... Kumar, Nrisingha Dey, and Indu Bhushan Maiti. "pSiM24 is a novel versatile gene expression vector for...

  2. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...can be used in combination to simultaneously perform modifications, simply by adding all the necessary ...allow any cloning procedure to be performed using a simple two-step, 2 hr protocol prior to transformation...linear fragments need to get into the same cell. For simple cloning requiring just one or two recombination...Subcloning - To move a gene from one vector to another simply amplify the vector and insert with separate primer...colonies. We routinely screen just two colonies for simple cloning procedures. Use high fidelity DNA polymerase...

  3. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ... cloning techniques with higher efficiency and simpler procedures have been developed. Some of these include... excessive time requirements. FastCloning is a simpler yet reliable cloning technique that was developed...2. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and ligation-independent...

  6. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ... have no fear - I’m here to introduce you to a simpler, yet more effective, CRISPR-based epigenetic approach...Sapozhnikov and Szyf 2021).    Figure 3: A simpler approach - dCas9 blocks DNMT1 methylation, leaving...blasticidin or puromycin resistance genes.  It’s that simple! Good luck and happy editing!    Thank you to...

  7. Genetic Code Expansion

    Type
    Collection
    ...Mammalian TAG Simon Elsaesser 140011 pAS_4xMx PylT_FLAG-Mx PylRS PylRS M. alvus CpK Mammalian TAG Simon Elsaesser... TAG Simon Elsaesser 140013 pAS_4xMx PylT C41CA_FLAG_Mx PylRS PylRS M. alvus Mammalian TAG Simon Elsaesser...TAG Simon Elsaesser 140023 pAS_4xMma PylT_FLAG-Mma PylRS AF PylRS AF M. mazei Mammalian TAG Simon Elsaesser...Mammalian TAG Simon Elsaesser 154763 pAS_FLAG-G1 PylRS G1 PylRS M. archaeon Mammalian TAG Simon Elsaesser ... TAG Simon Elsaesser 154768 pAS_4xG1 PylT FLAG-G1 PylRS G1 PylRS M. archaeon Mammalian TAG Simon Elsaesser...Mammalian TAG Simon Elsaesser 174896 pAS_4xMmaPylT(UUA)_EF1_MmaPylRS PylRS M. mazei Mammalian TAA Simon Elsaesser...Mammalian TGA Simon Elsaesser 174901 pAS_MmaPylT_EF1_NES-MmaPylRS WT PylRS M. mazei Mammalian TAG Simon Elsaesser...

  8. Cre-lox system

    Type
    Collection
    ...Khakh 105869 pEMS1925 iCre-ERT2 Simpson 105870 pEMS1725 iCre-ERT2 Simpson 106368 pCMV-Tag2B-NCre N-terminal... iCre with MCS for inserting promoter none AAV Simpson 49455 pSH62-EBD Cre-EBD - Estradiol-dependent control...with MCS for inserting promoter, WPRE none AAV Simpson 72255 pCDH-CB-iCre-P2A-tdTomato-T2A-Puro iCre and...to turn off the expression of one gene while simultaneously turning on another. The FLEx switch system ...activates shRNA expression, removal of EGFP; See also similar plasmids pSico PGK GFP and pSico PGK puro Lentiviral...off shRNA expression, removal of EGFP; See also similar plasmids pSicoR PGK puro , pSicoR-Ef1a-mCh-Puro... to eGFP, removes dsRed. See article for other similar plasmids. Retroviral Clevers 15912 CTV Cre activates...

  9. CRISPR Guide

    Type
    Collection
    ... every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became the most... change the genomic target of the Cas enzyme by simply changing the target sequence present in the gRNA...The requirements for a CRISPR knockout assay are simple. You’ll need a Cas enzyme and a gRNA specific to...AcrVA3.1, which inhibits both Cas12a and Cas3. Similarly, some Acr proteins are specific to a particular...editors are based on cytosine base editors and similarly work by first converting cytidine to uridine. ...repress gene expression in mice and human cells. The simplest dCas9-based activators and repressors consist ...imaging has numerous advantages, including the simplicity of gRNA design, programmability for different...

  10. Antibody Guide

    Type
    Collection
    ...assays that have multiple proteins to detect simultaneously. Conjugation The process of attaching signaling...conjugate or looking for antibodies validated for a similar assay type) and search again. After filtering, ..., directly compare the product information for similar antibodies, looking for ones that have identical...price, shipping availability, ease of purchase, or similar factors. Secondary antibodies Secondary antibodies...

  12. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...62201 Plant yes, cut S. pyogenes Hyg Chen pUC57-Simple-gRNA backbone 51306 Xenopus BsaI none S. pyogenes...Bacteria BsaI yes, activate S. pyogenes Marraffini pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) 47868 Mammalian... natural type II-A CRISPR arrays capable of simultaneously repressing multiple target genes in Escherichia...

  13. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...sequences can be designed using Benchling or using similar design tools and ordered as standard desalted oligonucleotides...A., Park, K. U., & Brzezinski, J. A. (2020). Simultaneous deletion of Prdm1 and Vsx2 enhancers in the ...

  14. Mammalian RNAi Tools

    Type
    Collection
    ...cleavage of the target. RNAi using siRNA follows a similar mechanism. Since it is pre-processed, it can bypass...., & Nemunaitis, J. (2009). siRNA vs. shRNA: Similarities and differences . Advanced Drug Delivery Reviews...

  16. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Allows for visualization of multiple proteins simultaneously with dual labeling when used with two substrates...by ACP Synthase, thereby permitting selective simultaneous labeling in a sample. Please see the note below...

  17. TALEN Guide

    Type
    Collection
    ...target sites. (B) A TAL effector nuclease (TALEN). Similar in principle to the ZF nuclease, the components...with ZF arrays, if not slightly lower. Figure 2: Simplified representation of the Voytas/Bogdanove Golden...

  18. Viral Vectors

    Type
    Collection
    ...delivered to a host and generate virus (right), similar to how natural viruses can be delivered to a host...Viruses used to direct these alternate functions simply have to harbor alternate viral genomes, such that...

  19. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Cas9 vector based on the Zhang lab's PX330 , simultaneously targeting the genomic site and the donor vector...Degron Tagging Masato Kanemaki's lab has developed a simple and scalable CRISPR/Cas-based method to tag endogenous...

  20. CRISPR References and Information

    Type
    Collection
    ...gene, as well as a target site common to a set of similar sequences. Organisms include human, mouse, rat...for NmCas9 and BsmBI site for cloning in gRNA: pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) PDF 47.5...

  21. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...based on shared characteristics and evolutionary similarity. These are grouped into two large classes based...conditions. Genome editing with CRISPR is not only simple, but also scalable. Multiple gRNAs targeting multiple...

  22. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...of genes and genetic elements. Here we present a simple methodology for CRISPR design, cloning, and delivery...plasmid ID 42230). This plasmid allows for the simultaneous expression of sgRNA and SpCas9, but does not...

  23. Zhang Lab CRISPR Page

    Type
    Collection
    ...-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (Ran et al., Nature Protocols...cancer modeling Using Cas9 mice, Platt et al . simultaneously modeled the dynamics of KRAS , p53 and LKB1...

  24. Bacterial Expression Systems

    Type
    Collection
    ...proper conditions and, epitope tags can be used to simplify the purification process. Using plasmids in this...arabinose binding protein (AraF) in the plasmid. Other similar reporters for detecting maltose can be found here...

  25. Immunology Research Plasmids and Resources

    Type
    Collection
    ...with sequence similarity 3, member C GS3786, ILEI FAM3D family with sequence similarity 3, member D EF7...FLJ16023, KIAA0832, NR3B3 FAM3B family with sequence similarity 3, member B 2-21, C21orf11, C21orf76, ORF9, PANDER...platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) FLJ12858...leukocyte protein of 76kDa) SLP-76, SLP76 LOC652578 similar to Fc fragment of IgG, low affinity IIIa, receptor...

  26. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...CMV ALS Sanford Simon 115331 pLNCX2-mCherry-CHMP2B CHMP2B mCherry CMV ALS Sanford Simon 115333 pLNCX2-mCherry-CHMP2B-siRNAres...Green 50487 pSLIK-NFLAG-hFUS FUS Flag, His TRE ALS Zissimos Mourelatos 51146 DsRed-p150 217-548 DCTN1 CMV ...-CHMP2B-siRNAres CHMP2B mCherry CMV ALS Sanford Simon 115523 MSCV-human CSF-1Rwt-IRES-GFP CSF1R Hereditary...pcDNA4/TO/GFP-GIGYF2 GIGYF2 GFP CMV Parkinson's Simon Bekker-Jensen 141323 pENTR-TARDBP-G287A TARDBP ALS...

  27. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Zap) New alternately colored FRET sensors for simultaneous monitoring of Zn(2)(+) in multiple cellular ...detect subthreshold membrane potential changes Simultaneous two-photon imaging of action potentials and ...

  28. Depositor Collections

    Type
    Collection
    ...mini-promoters to drive selective expression in the brain - Simpson Reagents for improved expression of recombinant...

  30. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Cas9 species = S. pyogenes (PAM = NGG) 51306 pUC57-Simple-gRNA backbone T7 BsaI In vitro transcription none...

  32. TALEN Engineering

    Type
    Collection
    ... transcriptional activator target sites and to simplify the construction of TALEN expression plasmids ...

  34. Antibody Plasmid Collection

    Type
    Collection
    ...recombinant antibody fragments in the form of scFvs A simple vector system to improve performance and utilisation...

  35. AAVED

    Type
    Collection
    ...experimental goals. How does this method compare to other similar methods? Where does this method fit in the big ...

  36. Plant Plasmids and Resources

    Type
    Collection
    ...system based on tobacco rattle virus (TRV) that simplifies gene silencing and genome editing logistics -...

  37. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ... enhancer. pAdx-CMV-LacZ is uniquely suited to simplify the cloning process as it can be used in blue-...

  38. Zebrafish Plasmid Collection

    Type
    Collection
    ...cumulative genome editing - Jay Shendure Lab Simultaneous single-cell profiling of lineages and cell types...

  40. Ras Pathway

    Type
    Collection
    ...proto-oncogene Raf-1 proto-oncogene RAL RALA RALB v-ral simian leukemia viral oncogene homolog (ras related) RALBP1...

  41. Lentivirus Plasmids

    Type
    Collection
    ...tracking of viral transduction. See article for more similar plasmids. Elledge 21373 pHIV-EGFP 3rd EF-1alpha...

  42. Tetracycline Inducible Expression

    Type
    Collection
    ...transcriptional activation domain from HSV (herpes simplex virus). In the absence of tetracycline, the tetR...

  43. COVID-19 Resources

    Type
    Collection
    ...top General Plasmid Tools SHERLOCK and DETECTR - Simple, portable, and inexpensive platforms to reliably...

  45. Retrograde AAV viral preps

    Type
    Collection
    ...pAAV-hSyn-Cre-P2A-dTomato Syn Cre expression with simultaneous dTomato expression Recombinases Larsen 107788...

  46. The Pleiades Promoter Project

    Type
    Collection
    ... Jones SJ, Holt RA, Goldowitz D, Wasserman WW, Simpson EM. A regulatory toolbox of MiniPromoters to drive...

  47. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...., Rodriguez-Rozada, S., Dieter, A., Pieper, F., Sims, R., Tenedini, F., Bergs, A. C. F., Bendifallah,...cascade of the intracellular part of the receptor, similar to what the endogenous binding partner would do...over when and where the receptor is activated.  Similarly, PTLs are special, covalently linked molecules... light exposure to activate them. PORTLs use a similar concept, except the ligand is attached to a protein...

  48. Plasmids for Stem Cell Research

    Type
    Collection
    ...generate iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem...

  50. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    ...Super-resolution techniques such as Structured Illumination (SIM), Single Molecule Localization (PALM/STORM), or Reversible... the world around us. Without contrast, objects simply vanish into noise. Due to its unrivaled contrast...Fluorescent Widefield Microscopy is the most common and simplest form of fluorescent microscopy. Collimated (non-converging...thicker three dimensional sample (see Figure 1); similar to an MRI or CT scanner. This is accomplished by...penetrates deeper into biological tissue. To prove this, simply hold a flash light to the palm of your hand. Although... excited at one time, limiting photobleaching. Similar to widefield, the entire field of view is excited...Website Other major microscopy manufacturers have similar information References 1. Lichtman JW, Conchello...
Showing: 1 - 50 of 649 results