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We narrowed to 718 results for: des.1

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  1. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense) complex, comprised of a combination of Cas5...endogenous repair mechanisms. Cas3 belongs to the Class 1 family of CRISPR systems, the most abundant type found...bacteria and archaea. While abundant in nature, Class 1 systems are largely underutilized compared to their...still separate the individual Cas components. Figure 1: Overview of Cascade-Cas3 mechanism. Created with ... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

  2. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Fawzi 127194 RP1B FUS 1-163 QQ4xSS #1 FUS His T7 ALS Nicolas Fawzi 127195 RP1B FUS 1-163 QQ4xSS #2 FUS His... type 1 scFv [L24/1] ITPR1 CMV Spinocerebellar ataxia James Trimmer 206790 IP3 receptor, type 1 scFv [... pLKO.1 mTagBFP2 rat DJ-1 shRNA PARK7 U6 Parkinson's Timothy Ryan 223563 pmCherry-Synaptojanin-1-145 SYNJ1... His, V5 EF-1 alpha Parkinson's Mark Cookson 25082 pDEST51-LRRK2-R1441G LRRK2 His, V5 EF-1 alpha Parkinson's... His, V5 EF-1 alpha Parkinson's Mark Cookson 29399 pDEST51-LRRK2-I1371V LRRK2 His, V5 EF-1 alpha Parkinson's...pcDNA3-FLAG-Synaptojanin 1-145 SYNJ1 Flag CMV Parkinsonism, early-onset Pietro De Camilli 22292 pcDNA3-FLAG-Synaptojanin...-FLAG-Synaptojanin 1-170 SYNJ1 Flag CMV Parkinsonism, early-onset Pietro De Camilli 22293 pEGFPC1-FLAG-Synaptojanin...

  3. Immunology Research Plasmids and Resources

    Type
    Collection
    ...IGHDY1 IGHD1-1 immunoglobulin heavy diversity 1-1 IGHD11 IGHD1-14 immunoglobulin heavy diversity 1-14 (non-...IGHV6-1 immunoglobulin heavy variable 6-1 IGHV61, VH IGHV7-4-1 immunoglobulin heavy variable 7-4-1 IGHV7... TAPBP-R, TAPBPR THBS1 thrombospondin 1 THBS, THBS-1, TSP, TSP-1, TSP1 TRPC4AP transient receptor potential...variable 2-8 IGLV28, V1-2 IGLV3-1 immunoglobulin lambda variable 3-1 IGLV31, V2-1 IGLV3-10 immunoglobulin lambda...NCC-1, NCC1, SCYA13, SCYL1 CCL14 chemokine (C-C motif) ligand 14 CC-1, CC-3, CKb1, FLJ16015, HCC-1, HCC... 3 G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3 CCL3L1 chemokine (C-C motif) ligand 3-like 1 464.2, D17S1718...GPD, GpFy, WBCQ1 DEFA1 defensin, alpha 1 DEF1, DEFA2, HNP-1, HP-1, MGC138393, MRS DEFA3 defensin, alpha...

  4. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Function PI AV-1-49531P 100040-AAV1 pAAV.hSyn1.Twitch2B.WPRE.SV40 Biosensor Oliver Griesbeck AV-1-50942 50942...Tobias Rose AV-1-PV2723 98929-AAV1 pAAV.hSyn.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-1-PV2724 98931... Looger AV-1-PV2725 98932-AAV1 pAAV.CAG.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-1-PV2816 100835... Kim AV-1-PV2817 100839-AAV1 pAAV.CAG.Flex.GCaMP6m.WPRE.SV40 Biosensor GENIE, Douglas Kim AV-1-PV2818 ... Kim AV-1-PV2819 100833-AAV1 pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 Biosensor GENIE, Douglas Kim AV-1-PV2820 ... Kim AV-1-PV2821 100845-AAV1 pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 Biosensor GENIE, Douglas Kim AV-1-PV2822 ...Douglas Kim AV-1-PV2823 100841-AAV1 pAAV.Syn.GCaMP6m.WPRE.SV40 Biosensor GENIE, Douglas Kim AV-1-PV2824 100843...

  5. Optogenetics AAV Preps

    Type
    Collection
    ...Constitutive 1, 9 Karel Svoboda 83898 pAAV-mDlx-ChR2-mCherry-Fishell-3 Dlx ChR2 mCherry Constitutive 1, 9, rg...Constitutive 1, 5, rg* Edward Boyden 59171 pAAV-Syn-ChrimsonR-tdT Syn ChrimsonR tdTomato Constitutive 1, 5, 9...Constitutive 1, 5 Ofer Yizhar 198511 pAAV_hSyn-DIO-PdCO-mScarlet-WPRE Syn PdCO mScarlet Cre dependent 1, 5 Ofer...Hillel Adesnik 170163 pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3 CAG ChroME2s mRuby3 Cre dependent 1 Hillel...pAAV-S5E2-ChR2-mCherry nEF ChR2 mCherry Constitutive 1, 9, PHPeB Jordane Dimidschstein 137142 pAAV-nEF-Con...-WPRE-HGHpA EF1a ChR2/H134R mCherry Cre dependent 1, 5, 8, 9, rg* Karl Deisseroth 20298 pAAV-EF1a-double...EYFP-WPRE-HGHpA EF1a ChR2/H134R EYFP Cre dependent 1, 5, 9, rg*, PHPeB Karl Deisseroth 26969 pAAV-CaMKIIa-hChR2...

  6. AAV Molecular Tools

    Type
    Collection
    ...Syn-driven, Cre-dependent Beta-Secretase to be expressed (1:1) with either ATLAS protein. 8 Don Arnold Don’t See...bicistronic expression of designer pro-taCasp3 and TEVp for studying cell ablation. 1, 5, 8 Nirao Shah , Jim...positive feedback loop for amplified tTA expression. 1 Viviana Gradinaru 99121 pAAV-ihSyn1-DIO-tTA Cre-dependent...positive feedback loop for amplified tTA expression. 1 Viviana Gradinaru 117383 TRE-DIO-eYFP Cre-dependent... Cre-dependent, Tet-inducible expression of EYFP. 1 Viviana Gradinaru Tools for Affinity Purification ...synaptophysin-EGFP for labeling of axon terminals. 1 Hongkui Zeng 71760 pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby...synaptophysin-mRuby for labeling of axon terminals. 1 Liqun Luo 60658 pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato...

  7. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...accumulation in bacteria. Biotechnol Biofuels. 2008 Jun 3. 1(1):11. Wolf Frommer Maltose Green fluorescent MBP-based...chloroplasts to nuclei provides a high-light signalling mechanism. Nat Commun. 2017 Jun 29;8(1):49. Philip Mullineaux...of cellular physiology. Nat Commun. 2020 Aug 4;11(1):3881. Adam Cohen Calcium GCaMP5 genetically encoded...in High-Ca 2+ Organelles. Cell Chem Biol. 2016 Jun 1. pii: S2451-9456(16)30163-5. Teresa Alonso , Javier... intracellular calcium. Nat Commun. 2021 Dec 9;12(1):7159. Dorus Gadella Calcium Teal genetically encoded...orange-emitting fluorescent proteins. Nat Commun. 2017 Sep 5;8(1):431. Wolf Frommer Calcium Orange fluorescent calcium...dynamics in mitochondria. Sci Rep. 2020 Feb 18;10(1):2835. Masamitsu Iino Calcium Red calcium sensor FRCaMPi...

  8. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...phosphosite library as GST-fusion peptides (“Mode #1” expression). The Mode #1 phosphosite library (Pooled ...References & Protocols Additional Resources Figure 1: SepRS adds a phosphoserine to tRNA^Sep with anticodon...standards for quantitative assessments. The Mode #1 phosphosite library can be generated with either phosphoserine...phosphorylation-dependent protein-protein interactions Figure 2: Mode #1 and Mode #2 phosphosite library configuration for ...Libraries #111705-8) . Screening kinases The Mode #1 phosphosite library can also be synthesized using ...iSPI_pSer_Subpool#2 Pooled Library 188526 iSPI_pSer_Subpool#1 Plasmid 112031 pNAS1b split mCherry 14-3-3β ctrl Plasmid... #2 Library (14-3-3β) Pooled Library 111704 Mode #1 Library Plasmid 69118 E17TAG GFP zeo resistance Plasmid...

  9. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...AdEasier®-1 cells (strain) - Bacterial strain that contains AdEasy®-1 plasmid...marker pLenti CMV/TO GFP-Zeo DEST (719-1) - 3rd gen lentiviral Gateway destination vector, expression, CMV/...GFP Varies pMKO.1 GFP - Retroviral shRNA expression pLenti CMV GFP DEST - ...N-terminal Myc tag for mammalian expression pGEX-4T-1-3xMyc - Bacterial vector for Myc tag pETcon(-) - Yeast...PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral shRNA expression Retroviral backbones...expression under a CMV promoter pAdEasy®-1 - Recombine plasmids from the shuttle... Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression...

  10. Fluorescent Proteins: FRET

    Type
    Collection
    ...EYFP 434 0.41 527 67,000 0.67 4.8 1.5 pmECFP-1 , pmEYFP-1 , pECFP18aaEYFP mCerulean mVenus 433 0.49 527...nm), ε A : Acceptor exctinction coefficient (M -1 cm -1 ), QY A : Acceptor quantum yield, R 0 : Förster...emission and acceptor excitation spectra (×10 15 M -1 cm -1 nm 4 ). Values obtained from FPbase.org FRET calculator... the donor lifetime. FRET is often used to study (1) protein-protein interactions where each protein is...maturation time, and other environmental factors. When designing your experiment, be sure to refer to published...proteins fused with linkers of varying lengths. Description Article ECFP-EYFP FRET standards, fusion proteins...Biosensor Database (Link opens in a new window) designed by Eric Greenwald and the Jin Zhang Lab at UCSD...

  11. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...10 Nucleus CAF-1 mGold2t Francois St-Pierre 231774 pCMV-mGold2s-CAF1-C-10 Nucleus CAF-1 mGold2s Francois...Brown 11908 pEGFP-N1 alpha-actinin 1 Actin filaments alpha-Actinin 1 EGFP Carol Otey 27676 Cortactin-pmCherryC1...Huttenlocher 11908 pEGFP-N1 alpha-actinin 1 Focal adhesions alpha-Actinin 1 EGFP Carol Otey 31923 pPaxillin-PSmOrange...Autophagosomes Sequestosome-1 EGFP Noboru Mizushima 38272 pMXs-IP GFP-WIPI-1 Autophagosomes WIPI1 EGFP ...apparatus eNOS(1-33) CFP Alexandra Newton 36205 pmTurquoise2-Golgi Golgi apparatus B4GALT1(1-61) mTurquoise2...mCherry-ZO1-C-14 Tight junctions Zonula Occludens-1 mCherry* Michael Davidson 55001 mCherry-Beta-Catenin... 30313 pEGFP ZO1 Tight junctions Zonula Occludens-1 EGFP Alan Fanning *Fusions to other FPs can be found...

  12. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Institute ID Tag Protein Structure 87420 PXN-EGFP AICSDP-1 EGFP Paxillin Matrix Adhesions 87421 TUBA1B-mEGFP ...TJP1-mEGFP AICSDP-23 mEGFP Tight junction protein ZO-1 Tight junctions 91565 AAVS1-mEGFP AICSDP-35 mEGFP ...Centrioles 101782 LAMP1-mEGFP AICSDP-19 mEGFP LAMP-1 Lysosome 101783 MAP1LC3B-mEGFP AICSDP-25 mEGFP Autophagy-related...101786 ST6GAL1-mEGFP AICSDP-26 mEGFP Sialyltransferase 1 Golgi 109122 NPM1-mEGFP AICSDP-50 mEGFP Nucleophosmin... HIST1H2BJ-mEGFP AICSDP-52 mEGFP Histone H2B type 1-J Histones 109119 CTNNB1-mEGFP AICSDP-47 mEGFP Beta-catenin...Mitochondria 87424 DSP-mEGFP AICSDP-9 mEGFP Desmoplakin Desmosomes 87425 ACTB-mEGFP AICSDP-15 mEGFP Beta-actin...human cell lines of their choice. For a full description of how to use these plasmids, check out Plasmids...

  13. Plasmids for Stem Cell Research

    Type
    Collection
    ... cell reprogramming factors and wait for cells to de-differentiate. However it may be difficult to decide... with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. Otonkoski Lentivirus Human Expression of human...cell reprogramming. Stem Cell Res Ther. 2017 Jun 5;8(1):132. Zovein Replicating EBNA1 episome Human Non-integrating...vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62. Jaenisch Lentivirus Mouse Doxycycline-inducible...transcription factors. Stem Cell Reports. 2015 Jan 13;4(1):25-36. Broccoli Fibroblasts Sensory Neurons Lentiviral...peripheral sensory neurons. Nat Neurosci. 2015 Jan;18(1):25-35. Baldwin Fibroblasts iTSCs Lentiviral Mouse... from Human ALS Patients. Cell Rep. 2016 Jan 5;14(1):115-28. Zhang Adult Dermal Fibroblasts Neurons Lentiviral...

  14. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...foreign DNA and cleaves it to destroy the invader ( Figure 1 ). Figure 1: An overview of the endogenous...separated by short palindromic repeat sequences. (1) The CRISPR array is transcribed to make the pre-CRISPR...engineering, with Type V following in 2015. Class 1 (Multi-subunit effector complex) Class 2 (Single multi-domain...enChIP) using CRISPR. Biochem Biophys Res Commun . 439(1):132-6. PMID: 23942116 Gilbert LA, Horlbeck MA, Adamson...X, Scott DA, Mikkelson T, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. 2014. Genome-Scale CRISPR-Cas9...CRISPR-Cpf1 using a single crRNA array. Nat Biotechnol . 35(1):31-34. PMID: 27918548...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows...

  15. AAV Packaged on Request

    Type
    Collection
    ...Small NEW 2 × 100 µL aliquots 0.2 mL 4 × 10 12 GC/mL* 1 × 10 13 GC/mL* Medium 10 × 100 µL aliquots 1.0 mL ...to 10 weeks. This breaks down as follows: Request 1–2 days Look for the banner on an eligible plasmid ...place your request. We will send you an email within 1-2 days with a response. Most requests will be approved... we can begin processing your order. MTA Approval 1+ business days, varies by requesting organization ...scientist through an MTA. This process typically takes 1–4 days, depending on your institution. AAV Production...all-in-one pricing for viral vector preps, which includes MTA facilitation, DNA amplification, and high-...

  16. Adenovirus Plasmids

    Type
    Collection
    ... Vogelstein 16399 AdEasier-1 BJ5183 derivative that contains the pAdEasy-1 (Addgene #16400) plasmid Vogelstein...vector production. ID Plasmid / Kit Description PI 16400 pAdEasy-1 Adenoviral vector for use in AdEasy...recombination with the shuttle plasmid. This collection provides a curated selection of popular plasmids that can...for shuttle plasmids with inserts. ID Plasmid Description PI 16402 pShuttle For production of viruses containing... for adenoviral vector production. ID Strain Description PI 16398 BJ5183 E. coli strain for recombination...

  17. CRISPR Guide

    Type
    Collection
    ...M. M., Semenova, E., Severinov, K., De Vos, W. M., Dame, R. T., De Vries, R., Brouns, S. J., & Van Der...changing the target sequence present in the gRNA. Figure 1: Overview of the basic CRISPR mechanism. CRISPR was...from Staphylococcus aureus ) has a coding sequence ~1 kb shorter than SpCas9 while retaining the same basic...lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included in the gRNA-containing...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense) complex to initiate DNA degradation. Cascade...Cascade C RISPR- as sociated c omplex for a ntiviral de fense; a complex of multiple Cas enzymes including...efficiency and fidelity. Nature Communications , 13 (1), 1425. PMID: 35301321 Edraki, A., Mir, A., Ibraheim...

  18. Zhang Lab CRISPR Page

    Type
    Collection
    ...Heidenreich 1, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33(1):102-6...SpCas9 alone without sgRNA. Full references are below. 1. SpCas9 (or SpCas9n, D10A nickase) + single guide ...screens. The libraries are available in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together..., Scott DA, Mikkelsen TS, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. Science . 2014 Jan 3;343(6166...):186-91. doi: 10.1038/nature14299. Epub 2015 Apr 1. PubMed . Return to AAV In vivo plasmids AAV Plasmids...oligos (design is indicated below) can be cloned into the CRISPR array. The oligos are designed based on...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids...

  19. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense), and four transposase components (TnsA, TnsB...some mammalian systems have been developed. Figure 1: Overview of the basic CAST mechanism. Created with... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

  20. Lentiviral Prep Service

    Type
    Collection
    ...version 1 of the CellTag barcoding library. Version 1 of the Celltag library contains 19973 barcodes to combinatorially...Barcoding Libraries ID Name Description PI 115643 Morris CellTag Barcode Library Version 1 Ready-to-use lentiviral... Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled...Version 2 of the Celltag library contains 4934 barcodes to combinatorially index cells for single-cell...Version 3 of the Celltag library contains 5737 barcodes to combinatorially index cells for single-cell...

  21. Viral Vector Guides and Plasmids

    Type
    Collection
    ...integration. Introduction to Viral Vectors Figure 1: Wild-type virus propagation and use of viral plasmids...viral vector guides and plasmids available at Addgene and other related resources. Science... Science Guides Viral Vectors...Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral Vectors Resources...selection of in-stock viral preps available. Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral...the production of viral vectors that contain the desired genetic cargo. In turn, these viral vectors are...

  22. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... orientation of each target site (see Figure 1). Figure 1: Recombination outcomes are determined by target...minimal FRT GAAGTTCCTATTCtctagaaaGTATAGGAACTTC Table 1. Summary of Cre, Dre, and Flp Recognition Sites. Controlling...target site sequence for your recombinase (see Table 1) to Search by Sequence to find other Cre-, Flp-, or...efficiency, selecting desired clones, and making transgenic mice. ID Plasmid Description Gene/Insert Vector...light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources...Return to top Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources...Return to top Dre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources...

  23. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Link opens in a new window) References Botman, D., de Groot, D. H., Schmidt, P., Goedhart, J., Teusink,...fluorescent proteins in budding yeast. Sci Rep, 9 (1):2234. https://doi.org/10.1038/s41598-019-38913-z ...

  24. Mammalian RNAi Tools

    Type
    Collection
    ...more information on shRNA design and delivery, see resources below. Figure 1: Overview of shRNA-mediated...Publication Additional Resources Addgene Resources pLKO.1 cloning vector protocol Other Addgene viral protocols...target sequence and RISC cleaves and degrades the mRNA. RISC includes argonaute protein 2 (Ago2), which ...RISC). RISC unwinds the double-stranded siRNA and degrades one of the strands. The remaining single-stranded... Trono Lab Lentivectors Toolbox Optimized shRNA design in pSico with pSicoligomaker (Andrea Ventura lab...Taxman, D. J. (2010). Short hairpin RNA (shRNA): design, delivery, and assessment of gene knockdown . Methods...

  25. Tetracycline Inducible Expression

    Type
    Collection
    ...system. ID Plasmid Description Transactivator PI 26429 pLenti CMV rtTA3 Blast (w756-1) Lentiviral Tet-On...and releases tet O, enabling transcription (Figure 1). While TetR and tet O could be a basic tool to control...skip ahead to view highlighted Tet plasmids . Figure 1: Tet-regulated expression systems. Left: natural TetR...though the DNA-binding profile is reversed (Figure 1). However, rtTA can induce much stronger expression...Mikhail Alexeyev 17492 pLenti CMV TetR Blast (716-1) Lentiviral Tet-On vector expressing TetR from CMV...promoters (sometimes generally called pTet) have been designed by combining tet O sequences with elements like...plasmids use "TetO" or "Tet-O" in their name or description, meaning they contain a TRE or tet O and can ...

  26. Brain Initiative Collection

    Type
    Collection
    ...element Gordon Fishell 83900-AAV1 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV2 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV5 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV8 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV9 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons... Gordon Fishell 83900-AAVrg pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons... Gordon Fishell 83900-PHPeB pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...

  27. AAV for Neuronal Tracing

    Type
    Collection
    ...rabies virus (RABV) for monoclonal neuronal tracing. (1) AAV encoding TVA and G-protein is delivered to the...with a deletion-mutant rabies virus. Nat Methods. 4(1):47-9. PMID: 17179932 Wickersham IR, Lyon DC, Barnard...Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G...

  28. Lentivirus Plasmids

    Type
    Collection
    ...gRNA expression . ID Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid...Weinberg 10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid. Includes a stuffer for easy...coexpression. Didier Trono 17619 EF.CMV.RFP 2nd EF-1 alpha promoter for transgene and CMV drives expression... Linzhao Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with constitutive or inducible...Fraser 15948 pLOVE 3rd Tet inducible gateway destination plasmid for cDNA expression. Miguel Ramalho-Santos...Tet-inducible lentivirus for ORF expression, multicistronic design enables tracking of viral transduction. See plasmid... based) for inducible knockdown, multicistronic design enables tracking of viral transduction. See Meerbrey...

  29. Antibody Plasmid Collection

    Type
    Collection
    ...phage display. J Immunol Methods. 2000 Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression...immunostaining. Biochem Biophys Res Commun. 2015 Jan 2;456(1):527-33. Gavin Wright pET-30-based vector dedicated...create and express plasmid-based antibodies. Description Article PI One-step assembly of antibody heavy...for "anti-mouse" or "anti-rabbit") ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions...

  30. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Facilitate the Itch-Scratching Cycle via Descending Regulation. Neuron 101(1):45-59. PubMed (Link opens in a new...populations defined by multiple parameters. Figure 1: Examples of intersectional cell population definitions... as a proof-of-concept targeting approach in 2014 1 (using EYFP and ChR2-EYFP as payloads). This approach...interneurons encode fear memory. Nat Neurosci. 23(1):61-74. PubMed (Link opens in a new window) Hafner... experimental design. How-to guide (Link opens in a new window) for the molecular design and testing of...interesting in implementing intersectional experimental design. The Deisseroth Lab maintains a Standard Operating...

  31. Genetic Code Expansion

    Type
    Collection
    ...Bacterial TAG Farren Isaacs 73545 pEvol-pAcFRS.1.t1 pAcFRS.1.t1 E. coli p-acetyl-l-phenylalanine (pAcF) Bacterial...Bacterial TAG Farren Isaacs 73547 pEvol-pAzFRS.1.t1 pAzFRS.1.t1 E. coli p-azido-l-phenylalanine (pAzF) Bacterial...recognized by the cognate orthogonal tRNA. Figure 1: Use of synthetase and target plasmids in genome code...7FTrp) Bacterial TAG Thomas Huber 207639 pEVOL-NBK-1 PylRS M. mazei Pyrrolysine Bacterial Cole DeForest...and tyrU gene replaced by GentR) and release factor 1. Expresses archaeal MjTyrRS/tRNA pair instead. For...genetic code for all life is based upon four nucleotides, 64 codons, and 20 amino acids. Scientists have...included. For any and all questions you have while designing, implementing, or troubleshooting GCE experiments...

  33. Rett Syndrome

    Type
    Collection
    ...affecting females at an incidence of approximately 1:10,000. It is characterized by apparently normal development...in transcriptional repression, activity dependent de-repression, chromatin remodeling, gene activation... the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator of Retinoic acid and Thyroid hormone...species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External...BrainSpan Atlas of the Developing Human Brain - includes developmental transcriptome information, prenatal...

  34. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...small enough to be packaged in AAV particles. Figure 1: Overview of RNA targeting with CRISPR. Browse, sort...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR...mutation reduces off-target effects of ADAR2 by destabilizing its RNA binding. Editors carrying the delta-...or search the tables below for CRISPR plasmids designed for RNA editing. To learn more about RNA editing... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

  35. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ..., or the beginning of the coding sequence. Figure 1: Overview of CRISPR interference. Browse, sort, or... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

  36. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ... assembly reaction** 1 5 min., 37 ºC (2.5 μl) 687 100% 274,200 2,742,000 1 60 min., 37 ºC (2.5 μl)...expected complete sequence for the lacI/lacZ genes (1), while sequencing of white colonies showed a mixture...representative transformation plates obtained from 1-, 12- and 24-fragment assemblies of the lacI/lacZ ...lacZ cassette, and illustrates how the volume of the 1 ml outgrowth spread on each transformation plate can...fidelity studies conducted by Potapov et al. at NEB (1, 2). While T4 DNA Ligase, the mainstay of most biotechnological...over 90% for 24-fragment assemblies (Figure 3, Table 1). These improvements (5- to 12-fold increases in the... 16 ºC) x 30 (100 μl) 78 90.7% 783 9,792 Table 1. Yields and fidelities for Golden Gate Assemblies ...

  37. Plan Your Experiment

    Type
    Collection
    ...start designing the specific components (Cas enzyme and guide RNA) for your experiment (Figure 1). You...gRNA), the design of the gRNA sequence, and finally how to validate your genetic edit. Figure 1: Flow chart...population. Some cells may remain wild type due to either (1) a lack of gRNA and/or Cas9 expression or (2) a lack...Biology , 1239 , 197–217. https://doi.org/10.1007/978-1-4939-1862-1_10 PMID: 25408407 Dixit, A., Parnas, O...used for gRNA design. Read more about how to design your gRNA . Synthesize and Clone Desired gRNAs Once ...multi-plasmid SAM system Activate Design and Clone Your gRNA Achieving your desired edit comes down to location...on proper gRNA design. While there are some cases where it makes sense to manually design a gRNA, in most...

  38. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...also indirectly modify local chromatin state. Figure 1: Overview of epigenetic modification using CRISPR....or search the tables below for CRISPR plasmids designed for epigenetic modification. To learn more about... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

  39. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...64325 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO-1 Fujii Efficient isolation of specific genomic regions...CRISPR plasmids that have been designed for use in bacteria. CRISPR...Resources The following CRISPR plasmids have been designed for use in bacteria. To find emtpy gRNA plasmids... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

  40. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...the dCas9-activator to your specific locus. Figure 1: Overview of CRISPR activation. Created with BioRender.com... can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

  41. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...collateral RNA degradation seen in bacteria. Figure 1: Overview of RNA targeting. Browse, sort, or search...regardless of homology to the crRNA. This activity provides a stark contrast to Cas9 and Cas12, which require...search the tables below for CRISPR plasmids designed for RNA targeting. To learn more about RNA targeting... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

  42. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...-Halo tag - Mammalian Gateway Destination Vector pENTR4-HaloTag (w876-1) - Gateway Entry Vector pCS2+/...Expression mEGFP 488 507 34 6 Monomer (A206K) pmEGFP-1 - Mammalian Expression mEGFP-N1 - Mammalian Expression...TurboRFP-pBAD - Bacterial Expression tdTomato 554 581 95 4.7 1 h Tandem-dimer pCSCMV:tdTomato - Mammalian Expression...-pBAD - Bacterial Expression mRFP1 584 607 13 4.5 1 h Monomer pcDNA3-mRFP - Mammalian Expression pMXs-...pSNAPf-C1 - Mammalian Expression pENTR4-SNAPf (w878-1) - Gateway Entry Vector pCS2+/C-SNAPf , pCS2+/N-SNAPf...fluorescence to reach half its maximal value. Structure describes the oligomeric state of the protein. Many FPs ...

  43. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...in mammalian cells or zebrafish). pMG10 (a '“2-in-1” ZFN cassette for expression of both a “+” and “-”...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays...Expression Vectors This page lists plasmid vectors designed to express nucleases in different cell types. ...zebrafish (can be used as a substitute for pMLM335 described in Foley et al., PLoS One 2009) pMLM292 (for expression...zebrafish (can be used as a substitute for pMLM336 described in Foley et al., PLoS One 2009) pMLM800 (for expression...Methods 2011 ) in mammalian cells or zebrafish as described by Moore et al. PLoS One 2012 and Cade et al.,...

  44. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...for any application TMN200 (200 mM sodium chloride, 1 mM magnesium chloride, 20 mM Tris at pH 8.0) Suitable...adeno-associated viral vector. Hum Gene Ther Methods . Feb;25(1):72-82. PMID: 24191859 Ye, et al. 2015. Safety and...name for ordering information. ID Name Promoter Description Category PI 157970 pTR-UF11 chimeric CMV/Chicken...

  45. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ... edits via homology-directed repair (HDR). Figure 1: Overview of Cas9 nickase cleavage and repair. Browse...PI Publication Return to top Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

  46. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...with a genomic region of interest in vivo. Figure 1: Overview of purification of genomic loci using CRISPR...or search the tables below for CRISPR plasmids designed for the purification of genomic loci. To learn... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

  47. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...marker. Looking at Table 1, we can recognize a couple of fluorophores from Figure 1. For example, the chart...brightness of individual fluorophores. Table 1 describes the brightness of a few commonly used fluorophores...efficient (i.e., happen most of the time). Figure 1 shows a graph from the BD® Spectrum Viewer, outlining...Resonance Energy Transfer (FRET).   Figure 1: Emission spectra and respective colors of a few commonly...Brighter fluorophores have a stronger signal. Table 1: Degrees of brightness for a few commonly used fluorophores...blue laser excites at a wavelength of 488 nm (Table 1), which is near a local maximum of PE's excitation...Knowing that the red laser excites at 640 nm (Table 1), while comparing the two fluorophore's excitation...

  48. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...with target sites throughout the chromosome. Figure 1: Overview of visualizing genomic loci using CRISPR...easy to implement due to the simplicity of gRNA design, programmable for different genomic loci, capable...or search the tables below for CRISPR plasmids designed for the visualization of genomic loci. To learn... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

  50. Technical Design of a Western Blot

    Type
    Blog Post
    ...Tris-tricine 2.5–40 kDa Tris-tricine 30 V, 1 hour or 100 V, 1–2 hours Good separation, quality, and stability...dive in!      Figure 1: The technical decisions to make when designing a western blot.  Psst!...assay. The most common options are 4 °C overnight or 1–2 hours at room temperature (RT). Most people choose...overnight at 4 °C and their secondary antibody for 1–2 hrs at room temperature, which allows them to reduce...weight of the proteins you’re interested in. Table 1 lists the most common types of gels for SDS-PAGE running...gel chemistry and/or specialty gel options. Table 1: Types of gels  Gel Type Protein sizes Running... Tris-glycine 6–400 kDa Tris-glycine 100 V, 1–2 hours Easy and cheap to handcast Short shelf-life...
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