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We narrowed to 402 results for: CAPS;

Showing: 41 - 80 of 402 results

  1. Viral Production at Addgene

    Type
    Blog Post
    ...plasmid, a plasmid encoding rep and serotype-specific cap genes, and a plasmid encoding adenoviral helper sequences...intensity of the viral capsid proteins are analyzed. The abundance of viral capsid proteins as a fraction...the relative stoichiometric ratios of the viral capsid proteins VP1, VP2, and VP3. Samples of viral preparations...non-purified AAV subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are shown relative to...

  2. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...produce AAV: 1) the RepCap plasmid which provides the AAV replication (rep) and capsid (cap) genes. AAV replication...that's then packaged into AAV’s protein shell, or capsid; 2) the pHelper plasmid which expresses adenovirus...

  4. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Rep68, Rep52, and Rep40. Cap in trans The cap gene encodes three AAV viral capsid proteins VP1, VP2, and...Packaging plasmid (also known as Rep/Cap plasmid) — containing the Rep and Cap regions necessary for the production...replication and viral packaging. The Cap region encodes three structural capsid proteins or viral proteins (VP1...production, as it carries the Cap region responsible for the production of capsid proteins. AAV2 is the most...to create and test capsid libraries with random mutations or insertions in the Cap region. Several methods...and consists of two open reading frames, Rep and Cap, flanked by two 145 bp inverted terminal repeats ...constructs are designed by replacing the Rep and Cap regions between the two ITRs with a gene expression...

  5. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...database, which relies on CAGE-seq to directly capture the mRNA cap, provides the most accurate mapping (Radzisheuskaya...thus frameshifts that disrupt the protein-coding capacity of a locus. When using S. pyogenes Cas9 (SpCas9...gRNAs is important to consider. While the basic landscape of mismatches that can nevertheless still lead...

  6. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...culture to air and make sure to tighten the flask cap before placing them in the incubators. This will ...ensure complete sterilization as mycoplasma can even escape 0.2-micron filters. Figure 1: Contamination...easily visible on the agar plates. This method is capable of detecting most of the mycoplasma species, apart...be overcrowded (filled no more than 60% of its capacity) which can cause mycoplasma infection from one...

  7. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...Christopher D. Lima. "The polynucleotide ligase and RNA capping enzyme superfamily of covalent nucleotidyltransferases...with products identifiable and quantifiable by capillary electrophoresis (CE) (see below). This method ...novel 16S rRNA gene PCR-ligase detection reaction-capillary electrophoresis assay." Journal of clinical microbiology.../LDR/flow-through hybridization assay using a capillary tube, probe DNA-immobilized magnetic beads and...PMC1978145. 27. Greenough, Lucia, et al. "Adapting capillary gel electrophoresis as a sensitive, high-throughput...

  8. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...transcript begins at the beginning of R, has a 5' cap structure, and proceeds through U5 and the rest of...retroviral particle containing matrix, capsid, and nucleocapsid components. pol in trans Precursor protein...and gamma-retroviruses is that lentiviruses are capable of infecting non-dividing and actively dividing...shRNA of interest flanked by LTRs; ~8 kb packaging capacity Packaging plasmid — contains packaging genes gag...) in cis RNA packaging signal; recognized by nucleocapsid proteins and essential for efficient viral packaging...

  9. Antibody Guide

    Type
    Collection
    ...antibodies are capable of recognizing and binding to epitopes on antigens, they are also capable of recognizing...In a sandwich ELISA, a capture antibody, bound to a surface, is used to capture the protein of interest...also be included. Antibody Applications - Capture Methods Capture methods such as immunoprecipitation (IP...variation of IP uses antibodies to capture protein:DNA complexes. After capture, the DNA is released from the...fragmentation of the DNA. Controls for capture methods Capture methods should use a positive control and...methods. Capture MS validation This method uses peptide identification by proteomics (Capture MS) to validate...Amplification Application Overview Quantification Methods Capture Methods Visualization Methods Cell Sorting Methods...

  10. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ..., and dogs. This serotype was derived from AAV2 capsid and developed by Arun Srivastava’s lab. AAV2(trpYF...Arun Srivastava’s lab. It is derived from the AAV2 capsid and contains the following mutations: Y444F, Y500F...Arun Srivastava’s lab. It is derived from the AAV6 capsid and contains the following mutations: Y705F, Y731F...19066593 Other citations include: Bogner, et al. 2015. Capsid Mutated Adeno-Associated Virus Delivered to the...Kanaan, et al. 2017. Rationally Engineered AAV Capsids Improve Transduction and Volumetric Spread in the...al. 2016. Microglia-specific targeting by novel capsid-modified AAV6 vectors. Mol Ther Methods Clin Dev...

  11. COVID-19 Resources

    Type
    Collection
    ...targeting the SARS-CoV-2 nucleocapsid protein (Brian Geiss). Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] - ...monoclonal antibodies that target SARS-CoV-2 nucleocapsid protein. SARS-CoV-2 Plasmids : Plasmids that...COVID-19 research. Antibodies Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] - Clone mBG17, recombinant mouse...monoclonal antibody targeting the SARS-CoV-2 nucleocapsid protein (Brian Geiss). Plasmids SARS-CoV-2 Plasmids...RBD mutants that can be used to identify antibody escape mutants and for comprehensive mapping. Some of ...

  12. Viral Vector Guides and Plasmids

    Type
    Collection
    ...transgene, packaging capacity, and size. Vector Type Expression Genome Packaging Capacity Virus Size Cells...genome. AAVs are limited by their small packaging capacity. Gamma-retroviral Vector Guide | Gamma-retroviral...Plasmids Adenoviral vectors have high packaging capacity, high transduction efficiency, and high but transient...components of the viral genome to assemble the viral capsids (packaging and envelope plasmids). When transfected...

  13. Arf GTPase Family

    Type
    Collection
    ...pEGFP-C2) GAP Acap1 (CENTB1) 9744 740 Mammalian (pFLAG-CMV2) GAP Acap2 (CENTB2) 23527 778 GAP Acap3 (CENTB5)...Asap2 (PAG3, CENTB3, AMAP2) 8853 1006 GAP Asap3 (ACAP4, CENTB6) 55616 903 Bacterial (pET) GAP Arap1 (CENTD2...

  14. CRISPR Guide

    Type
    Collection
    ... others work to increase Cas9’s proofreading capabilities. No matter the method, increased fidelity enzymes...by the Feng Zhang lab ) combine the large-scale capacity of transposases with the precise DNA-targeting...programmability for different genomic loci, and the capability to detect multiple targets simultaneously. CRISPR...and biotin tags, or an anti-Cas9 antibody. The CAPTURE system, developed by the Jian Xu lab , utilizes... Cas12a also has more condensed multiplexing capabilities , as it can process multiple gRNAs under a single...carrying an ADAR truncation that retain RNA editing capabilities but are small enough to be packaged in AAV particles...two nuclease domains inactivated. This enzyme is capable of cleaving only one strand of target dsDNA. Off-target...

  15. Plasmids for Stem Cell Research

    Type
    Collection
    ...they are capable of differentiating into every other cell type in the body and have the capacity to give...are proliferative, unspecialized cells with the capacity to differentiate into many different cell types... Acad Sci U S A. 2014 Jul 22;111(29):10678-83. Capecchi Replicating EBNA1 episome Human Fluorescent-tagged...

  16. AAV Packaged on Request

    Type
    Collection
    ...sometimes require preps beyond our current in-stock capabilities. To meet that need, our Packaged on Request ...globally connected scientific community. The Data Hub captures valuable knowledge that often goes unshared, bridging...troubleshooting and results that complement published data, capturing the insights that often go unshared. Help Center...

  17. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...nickase fused to a reverse transcriptase (RT) is capable of installing targeted insertions, deletions, and... to immunoprecipitate FLAG-tagged Cas9. In the CAPTURE system, Cas9 is tagged with a biotin acceptor site...streptavidin affinity purification. For both enCHIP and CAPTURE, design your gRNA sequence to direct dCas9 to a...

  18. Immunology Research Plasmids and Resources

    Type
    Collection
    ...polypeptide 1 (pituitary) receptor type I PAC1, PACAPR, PACAPRI ADIPOQ adiponectin, C1Q and collagen domain...receptor 1 FLJ41949, HVR1, II, PACAP-R-2, RDC1, VAPC1, VIPR, VIRG, VPAC1, VPCAP1R VIPR2 vasoactive intestinal...C5R1, CD88 CAMP cathelicidin antimicrobial peptide CAP18, CRAMP, FALL-39, FALL39, HSD26, LL37 CCBP2 chemokine...MGC138386, NDI, V2R AZU1 azurocidin 1 AZAMP, AZU, CAP37, HBP, HUMAZUR, NAZC BDNF brain-derived neurotrophic...CGRPR, CRLR CAMP cathelicidin antimicrobial peptide CAP18, CRAMP, FALL-39, FALL39, HSD26, LL37 CAT catalase...intestinal peptide receptor 2 FLJ16511, VPAC2, VPCAP2R XCL1 chemokine (C motif) ligand 1 ATAC, LPTN, LTN, SCM...

  19. Lentiviral Vector Guide

    Type
    Guide
    ...begins, by definition, at the beginning of R, is capped, and proceeds through U5 and the rest of the provirus...lentiviral particle containing matrix, capsid, and nucleocapsid components. pol in trans Precursor protein...and gamma-retroviruses is that lentiviruses are capable of infecting non-dividing and actively dividing...of interest flanked by LTRs; 8–10 kb packaging capacity Packaging plasmid — contains packaging genes gag...) in cis RNA packaging signal; recognized by nucleocapsid proteins and essential for efficient viral packaging...

  20. Adenovirus Plasmids

    Type
    Collection
    ...adenoviruses in research include their large packaging capacity (>8 kb), high titers, and high levels of transgene...substantial immune response induced by the viral capsid. Adenoviruses can be produced by several methods...

  21. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Applications Scientists have also used the targeting capability of Cas9 to make other types of modifications ...ChIP (enChIP) or biotin-streptavidin pulldown (CAPTURE.) Tag : Multiple methods make it easier to tag ...

  22. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    .... Chen Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system Plant...modular assembly of natural type II-A CRISPR arrays capable of simultaneously repressing multiple target genes...

  23. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...984-1090 ADAR2 truncation retain RNA editing capabilities but are small enough to be packaged in AAV particles...

  25. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...Sequence (SITS). This sequence bypasses the 5′ mRNA cap required for eukaryotic systems and instead directly...goals of synthetic biology is to engineer cells capable of recording permanent "memories" of molecular ... sequences (pMemoryArray) gives a DNA sequence capable of recording 11 bits (1.375 bytes) of information....1420024112. Epub 2015 Feb 23. A novel dCas9 activator capable of unprecedented activation of target genes A group... Institute have extended the transactivating capabilities of CRISPR/dCas9-fusion proteins and have made...additional domains tested (VP64, p65, and Rta) were capable of increasing target gene expression, adding all...gene activation. The novel dCas9-VPR activator is capable of enhancing target gene expression anywhere from...

  30. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...nickase fused to a reverse transcriptase (RT) is capable of installing targeted insertions, deletions, and...

  31. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...terminus of Cas9 H840A nickase. The fusion enzyme is capable of installing targeted insertions, deletions, and...

  32. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...nickase fused to a reverse transcriptase (RT) is capable of installing targeted insertions, deletions, and...

  33. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ... WPRE has been removed to increase the cloning capacity. pCDH-CB-IRES-copGFP-T2A-Puro 72299 Express gene...

  34. CRISPR Plasmids - Plants

    Type
    Collection
    ...nickase fused to a reverse transcriptase (RT) is capable of installing targeted insertions, deletions, and...

  35. Lentivirus Plasmids

    Type
    Collection
    ...envelope plasmids) encode components of the viral capsid and envelope and are used in conjunction with the...

  37. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...vectors have both distinctive capsids and genomic structures. The viral capsid is the key that lets AAVs ...of promoter/enhancer. Table 1: AAV capsids and receptors. Capsid Primary receptor Coreceptor AAV1... true! In a previous post, we covered systemic capsids, which allow AAVs to broadly spread throughout ...particular cell type depends largely on the viral capsid, but specificity doesn’t end there: expression ...engineered serotypes by mixing, matching, and modifying capsid and genomic structures. For example, rAAV pseudotypes...pseudotypes mix the genome of one AAV serotype with the capsid of another. Formally, pseudotypes are described...refers to the genome, and the second number to the capsid. Most vectors provided by Addgene are pseudotypes...

  38. Rett Syndrome

    Type
    Collection
    ... MECP2 null alleles or common mutations that recapitulate Rett syndrome in humans. MECP2 knockout rats...

  39. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...-enveloped particle of ~100 nm, consisting of a capsid surrounding an inner core which contains the adenoviral...~ 100nm in size, and consists of an icosahedral capsid made of 3 types of proteins: fiber, penton and ...protected by histone-like proteins forming the nucleocapsid. B) Three generations of adenoviral vectors:... Ad vectors (helper-dependent (HD-Ad), or high-capacity (HC-Ad__ are devoid of viral genes and therefore...such as HEK293 cells. 1st generation AdV cloning capacity is limited to 8.2 kb, and in vivo transgene expression...Gen AdVs were designed to have increased cloning capacity, decreased  potential for the generation of RCA...2nd generation AdVs, thereby increasing cloning capacity to ~12 Kb. The ever popular pAdEasy system belongs...

  40. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...in this post: Pooled library to study antibody escape in SARS-CoV-2 Recombinant antibodies for aminoacyl...New ready-to-use viral preps Mapping antibody escape in SARS-CoV-2 by mutational scanning By Seth Kasowitz...SARS-CoV-2), the virus may acquire mutations to escape these therapeutics. Jesse Bloom’s lab at the Fred...From Starr et al Cell., 2020. (B) Mapping antibody-escape mutations with the yeast display library. From ...rate-limiting enzymes. In this method, the major capsid protein VP1 is co-expressed with a fusion protein...VP1, which self-assembles into a compartment encapsulating the protein of interest (Figure 3).   ...D-glucaric acid biosynthesis. This MPyV system encapsulated a rate-limiting enzyme in this pathway (myo-...
Showing: 41 - 80 of 402 results