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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 849 results for: HA

Showing: 41 - 80 of 849 results

  1. Chemogenetics Plasmids

    Type
    Collection
    ...on the ion pore domain it is coupled to. Addgene has two main types of PSAMs: 5HT3 coupled, which are ...

  3. Resolute Plasmid Collection

    Type
    Collection
    ...and pharmaceutical companies. The RESOLUTE project has received funding from the Innovative Medicines Initiative...

  4. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... protein of interest with known markers. Addgene has assembled a collection of fluorescent fusion proteins...protein of interest with a fluorescent protein that has a different emission wavelength. For example, if ...mScarlet-I Dorus Gadella 50057 pLYS1-FLAG-MitoGFP-HA Mitochondria MCU GFP Vamsi Mootha 49153 GFP-Mff Mitochondria-Outer...

  5. AAV for Neuronal Tracing

    Type
    Collection
    ... delivery by an AAV. Since this starter cell now has all the viral genes present, the virus can spread...

  7. Retrovirus Plasmids

    Type
    Collection
    ... expresses GFP. Also see pMIG-w , a variant that has WRE, a post-transcriptional regulatory element that...

  8. Caltech Systemic Capsids

    Type
    Collection
    ...used in LY6A-nonpermissive mouse strains. **AAV1-X1 has not been tested in rats. Abbreviations: AAV, adeno-associated...Enhanced CNS tropism exhibited by the PHP.eB serotype has been reported to occur by using the cellular receptor...

  9. Antibody Production

    Type
    Collection
    ...in one of its recommended assays. If an antibody has passed stability testing, it may ship at room temperature...

  10. Microbiology Resources

    Type
    Collection
    .... Looking for E. coli or yeast plasmids? Addgene has a wide variety of tools for work in these model organisms...

  11. Worm Expression Resources

    Type
    Collection
    ...Editing in Caenorhabditis elegans. A modified approach has also been developed by the Fire laboratory and described...

  13. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Express gene of interest from the CMV promoter. WPRE has been removed to increase the cloning capacity. pCDH-CB-IRES-copGFP-T2A-Puro...

  14. AAV Packaged on Request

    Type
    Collection
    ...range of experiments, both in vitro and in vivo. It has low immunogenicity, is easy to use, and can be safely...

  15. Brain Initiative Collection

    Type
    Collection
    ...support from the BRAIN Initiative below. Support has been identified by depositors and/or when BRAIN Initiative...Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn ...forebrain GABAergic interneurons. The enhancer core has 100% seq conservation between mouse and human Jonathan...

  16. Genetic Code Expansion

    Type
    Collection
    ...its cognate aminoacyl-tRNA-synthetase (aaRS) that has been evolved to incorporate non-canonical amino acids...aminoacylate the orthogonal tRNA. And the orthogonal tRNA has to bind to an unallocated codon. Therefore many controls....1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA Y306A/Y384F (AF) pyrrolysine (Pyl) tRNA synthetase...

  17. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...molecules in either of these types of studies Addgene has a collection of plasmids suitable for creating individual...

  18. Plasmids for Stem Cell Research

    Type
    Collection
    ...development. Unlike other cell types, each stem cell has the potential to either remain a stem cell or become... concerns related to embryonic stem cell use and has the major advantage that iPSCs can potentially be...

  19. Brain Armamentarium

    Type
    Collection
    ...forebrain GABAergic interneurons. The enhancer core has 100% seq conservation between mouse and human Jonathan...

  20. Cre-lox system

    Type
    Collection
    ...fluorescent labels that indicate if recombination has occurred, allowing for direct comparison of Cre+ ...optimize Cre expression once it is induced. Addgene has a wide variety of Cre recombinase plasmids. Special...rates of Cre-driven recombination. CREM (or Cre-M) has been engineered to contain an intron, preventing ...

  21. Lentivirus Plasmids

    Type
    Collection
    ...virus integrates randomly into the host genome, it has the potential for insertional mutagensis. The 2nd...

  22. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Addgene has assembled a collection of empty plasmid backbones with different fluorescent tags for you... that result in a diverse set of colors. Addgene has assembled a collection of empty plasmid backbones...

  24. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... can be used in cells or organisms in which Cas9 has been integrated into the genome. gRNA Empty Vectors...Marraffini pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) 47868 Mammalian U6 yes, cut N. meningitidis...

  25. CRISPR References and Information

    Type
    Collection
    ...cloning in gRNA: pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) PDF, 49 KB Vosshall and Matthews (Link opens...

  26. Rett Syndrome

    Type
    Collection
    ...Rett Syndrome and related MECP2 disorders. The RSRT has a comprehensive, strategic and aggressive plan to...

  27. Plan Your Experiment

    Type
    Collection
    ...NHEJ. How do you determine that your desired edit has occurred? The exact method necessary to validate ...

  28. Immunology Research Plasmids and Resources

    Type
    Collection
    ...beta - HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-... MHC-G HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-...MSTP084 HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-...

  30. Gibson Assembly Protocol

    Type
    Protocol
    ... extra part, one part has half of the antibiotic gene and the adjacent part has the other half. Any colonies...fully ligated double-stranded DNA molecule. This has proven to be an efficient and effective method for... to improve accuracy and efficiency. Each enzyme has a specific and unique function for the reaction: ...also sell ET SSB (Link opens in a new window) . NEB has other resources, such as a primer design tool (Link...

  31. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...continue towards a boil. Keep an eye on it the solution has a tendancy to boil over. Placing saran wrap over ...sit at room temperature for 20-30 mins, until it has completely solidified. Pro-Tip If you are in a hurry... loading and allows you to gauge how far the DNA has migrated; 2) it contains a high percentage of glycerol...with water to even out the staining after the gel has been run, just as you would if you had not added ...water and destain for 5 mins. Using any device that has UV light, visualize your DNA fragments. The fragments...

  32. Weighing Reagents Protocol

    Type
    Protocol
    ...weighed out Procedure The balance in the photo above has a capacity of 200 g, as indicated by the number above...determine if this is best. Check that the balance has the correct capacity for the material that you are...

  34. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...protective measures for each level, as each level has different safety requirements. BSL-1 is designated...is important! Disclaimer: The SOP presented here has been designed by the Addgene nonprofit plasmid repository...

  35. Centrifugation

    Type
    Protocol
    ...not require specific PPE, you never know what else has been placed in the centrifuge before you and may ...the centrifuge’s internal lid on the rotor (if it has one) and then close the external lid. Adjust the ...

  36. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...the future you can thaw a new tube that you know has only been thawed once before. Always do controls....want to see is that your vector + insert ligation has many more colonies than your vector alone ligation...

  37. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...starting this procedure, be sure that the cell pool has been sufficiently selected with the appropriate antibiotic... antibiotic so that every cell in the culture has, in theory, been transduced. There are various schools...regularly for transgene expression. This protocol has been optimized for adherent cells but can be modified...

  38. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...). Pro-Tip Add protease inhibitors to 1X if this has not already been done. We typically start with about... NanoDrop Spectrophotometer to see if the sample has reached the desired concentration. If the sample ... NanoDrop Spectrophotometer to see if the sample has reached the desired concentration. Gently transfer...

  39. AAV Production in HEK293 Cells

    Type
    Protocol
    ...clogging during harvesting. The addition of sorbitol has been shown to increase viral titers by up to 1.8-...10 min and then recheck the pH to ensure that it has not drifted. Filter the solution through a 0.22 μm...such as sorbitol and sodium bicarbonate, sorbitol has been found to increase viral titers by up to 1.8-...

  40. DNA Quantification

    Type
    Protocol
    ... it is possible that the difference between them has affected the accuracy of the NanoDrop. It is a good...
Showing: 41 - 80 of 849 results