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We narrowed to 933 results for: KIN

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Showing: 51 - 100 of 933 results

  1. Lentivirus Plasmids

    Type
    Collection
    ...Vector Guide Popular Lentiviral Transfer Plasmids Looking for lentiviral plasmids to express a CRISPR gRNA... ORF expression, multicistronic design enables tracking of viral transduction. See plasmid 46948 for additional...inducible knockdown, multicistronic design enables tracking of viral transduction. See Meerbrey et al. (2011... Reiser. Jakob Reiser 63586 psPAX2-D64V 2nd For making integrase-deficient lentivirus. David Rawlings ...

  2. Antibody Guide

    Type
    Collection
    ...important factor (for instance, looking for a different conjugate or looking for antibodies validated for...unused surface-binding sites in the wells with a blocking protein such as BSA, followed by several wash ... assay, called native ChIP, does not use cross-linking and instead relies on strong interactions between...the product information for similar antibodies, looking for ones that have identical features and epitopes...

  3. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... does not require a specific flanking sequence on the target RNA, making it a very flexible system. Other...rather than DNA, sometimes requiring a protospacer flanking sequence (PFS). In bacteria, Cas13 targeting also...):442-51. PMID: 23849981 Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. 1987. Nucleotide sequence...

  4. Plasmids for Stem Cell Research

    Type
    Collection
    ... vector for "hit and run" reprogramming of adult skin fibroblasts to induced pluripotent stem cells. Stem...non-integrating episomes to allow sorting and dosage tracking of reprogramming factors Fluorescent tagged episomals...Stem Cell. 2012 Apr 6;10(4):465-72. Schöler Adult Skin Fibroblasts Cholinergic Neurons Lentiviral Human...Cell Stem Cell. 2015 Nov 5;17(5):543-56. Buganim Skin Fibroblasts Motor Neurons Lentiviral Human Direct...

  5. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...finger nucleases (ZFNs) are effective tools for making targeted mutations in zebrafish. With support ...OZ526 OPEN OPEN tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527 and OZ528 OPEN OPEN...engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML et al....

  6. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Localization General Cytoskeleton Junctions Membrane Trafficking Additional Resources The subcellular localization... Protein Collection . Return to top Membrane Trafficking The secretory pathway is responsible for delivering...find markers for components of these membrane trafficking pathways and structures. ID Plasmid Structure...Fluorescent Protein Collection . Return to top Looking for more constructs or different fluorescent protein...

  7. Distribution to Industry

    Type
    Collection
    ...RESOLUTE collection AAV Packaging from Addgene Parkinson's Research from The Michael J Fox Foundation Search...Screening Messer and MJFF Created for research in Parkinson's Disease; For target validation and drug design...Recommended Applications PI Don't See What You're Looking For? Please let us know what you would like to ...

  8. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...events and the systemic effects of Cre toxicity. Leakiness Leaky expression, where the gene of interest is...populations. Read our blog post about how to minimize leakiness in experiments using AAV vectors. Highlighted ...transfection efficiency, selecting desired clones, and making transgenic mice. ID Plasmid Description Gene/Insert...

  9. Genetic Code Expansion

    Type
    Collection
    ...ncAA) in a protein of interest. Applications By making small changes in selected amino acids within a ...there are several things to consider. If you are working off of a previously established protocol, make ...PylT_FLAG-AbKRS-chIPYE chimeric PylRS Chimeric photo-crosslinking-lysine Mammalian TAG Simon Elsaesser 140021 ...and References GCE4All Research Center GCE4All: Making Genetic Code Expansion Accessible Using Phosphoserine...

  10. Luciferase Plasmid Collection

    Type
    Collection
    ...Renilla luciferase have short protein half-lives, making them useful as transcriptional reporters, but are...a secreted version of the protein is available, making it ideal for a variety of applications. To learn...expressed from a T7 promoter for normalization. John Atkins 174050 pGWB-nLUC Firefly Creation of N-terminal...

  11. Bacterial Expression Systems

    Type
    Collection
    ...NusA Cleavage sites: TEV protease, factor Xa, enterokinase, thrombin Signal peptides for periplasmic localization...Fluorescent Dyes and Ligands GFP Fusion Proteins — Making the Right Connection Photoactivatable Fluorescent... Engineering Modifying the bacterial genome by knocking out genes or introducing specific mutations can...

  12. Plan Your Experiment

    Type
    Collection
    ...setting up CRISPR experiments. We will focus on making single edits using CRISPR/Cas9 in mammalian cells... the cell type. Before proceeding, we recommend asking labmates/colleagues, searching the literature, ...efficiency. Edit Type The type of edit you are looking for will ultimately depend on which CRISPR method...

  13. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...red over time, allowing tracking of processes such as intracellular trafficking or cell cycle dynamics....concentrations, so this is mutation is noted when present. Looking for more empty backbones? Visit our Empty Backbones...

  14. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system. ...

  15. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...viral proteins as a first step for many different kinds of important experiments. In an effort to speed ...Codon-optimized expression (recode) Truncated for making pseudovirus (Ctrunc) ID Plasmid Description Mutations...

  16. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...plasmids and backbones with high cloning potential. By making these plasmids available as cgDNA, we hope to aid...assistance at [email protected] . Don’t See What You’re Looking For? At this time, Addgene has made a limited number...

  17. Plasmid Collections

    Type
    Collection
    ... page to help you find what you're looking for. If you are looking for a non-plasmid item, browse our ...

  18. Optogenetics Plasmid Collection

    Type
    Collection
    ...engineered or identified to increase the spectral and kinetic properties as well as improve the expression and...ChR mCherry", "Cre inhibition", or other terms. Looking for viral preps? We also offer ready-to-use AAV...

  19. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...is a unique member of the CRISPR family. After nicking the target DNA, Cas3 initiates single-strand DNA...target DNA using a gRNA and recruits Cas3. After nicking the non-targeted strand, Cas3 starts unraveling...

  20. Chemogenetics Plasmids

    Type
    Collection
    ...luciferin substrate or by external illumination, making it a bimodal opto-chemogenetic actuator. For more..."KORD AAV", "LMO", "Sternson", or other terms. Looking for viral preps? We also offer ready-to-use AAV...

  21. Resolute Plasmid Collection

    Type
    Collection
    ...Initiative 2 Joint Undertaking under grant agreement No 777372. This Joint Undertaking receives support ...

  23. Synthetic Biology - Overview

    Type
    Collection
    ...Depositing Labs Hal Alper J. Chris Anderson Adam Arkin Gabor Balazsi Matthew Bennett Carolyn Bertozzi Nicolas...biological systems for novel functions. Addgene is working with leading synthetic biologists around the world...

  24. Microbiology Resources

    Type
    Collection
    ...accordance with export laws and biosafety regulations. Looking for E. coli or yeast plasmids? Addgene has a wide...Engineering blog post to see how Addgene depositors are making this possible in a range of organisms. Find CRISPR...

  25. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ... Mitochondria 87424 DSP-mEGFP AICSDP-9 mEGFP Desmoplakin Desmosomes 87425 ACTB-mEGFP AICSDP-15 mEGFP Beta-actin...protocol videos available that may be helpful for working with this plasmid collection. Single cell passaging...

  26. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Professor Oka and the Gene Vector Core have also kindly provided the following protocol detailing the process...gene of interest from the PGK (phosphoglycerate kinase I) promoter pCDH-CMV4 72284 Express gene of interest...

  27. AAV Molecular Tools

    Type
    Collection
    ...hSyn-driven, constitutive Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free...ATLAS protein. 8 Don Arnold Don’t See What You’re Looking For? Our Packaged on Request service offers you...

  28. Open Enzyme Collection

    Type
    Collection
    ...Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain) 165566 pOpen-TthRT Thermus thermophilus...methyltransferase 165520 pOpen-T4 PNK T4 PNK polynucleotide kinase 165521 pOpen-lambda red operon lambda red operon...

  29. Rett Syndrome

    Type
    Collection
    ...reverse the impacts of this disease. Addgene is working with the RSRT along with individual laboratories...Gribnau Xist 2lox/2lox Conditional Xist, Lox sites flanking exon 1,2,3 C57BL/6 Mouse line with conditional...

  30. Deisseroth INTRSECT Collection

    Type
    Collection
    ...opens in a new window) with general principles for working with INTRSECT recombinases, viruses, and important... N. 2019. Excitation of diverse classes of cholecystokinin interneurons in the basal amygdala facilitates...

  31. The Pleiades Promoter Project

    Type
    Collection
    ... Chen, J., Chen, Y., Cheng, J. C., Chopra, V., Docking, T. R., Dreolini, L., D'Souza, C. A., Flynn, E.... A., Turner, J. L., Varhol, R., Vermeulen, J., Watkins, R. F., Wilson, G., Wong, B. K., Wong, S. H., Wong...

  32. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...pAAV-AiE2150m-minBG-SYFP2-WPRE3-BGHpA AiP1379 AiE2150m SYFP2 Purkinje cells Cerebellum 208141 pAAV-AiE0390m_3xC2-minBG-SYFP2...Hooper, M., Omstead, V., Vargas, S., Lerma, M. N., Taskin, N., Weed, N., Laird, W. D., Bishaw, Y. M., Bendrick...

  33. Tetracycline Inducible Expression

    Type
    Collection
    ...normal conditions, the TetR protein binds to tet O, blocking transcription of the downstream gene. If tet or...our collection for all dox-regulated plasmids . Looking for viral preps? Find in-stock viral preps of tetracycline...

  35. Antibodies

    Type
    Collection
    ...Instructions Addgene Help Center Antibody Protocols Looking for Antibody Plasmids? Browse our collection of...

  36. Depositor Collections

    Type
    Collection
    ... from depositors. To help you find what you're looking for, we've gathered our depositor collections on...

  37. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...repression through cytosine methylation with improved kinetics LSD1 — repression through targeted removal of ...

  43. Serotype Testing AAV

    Type
    Collection
    ...why do I have to order it? Don’t See What You’re Looking For? Our Packaged on Request service offers you...

  45. Recombinases AAV Preps

    Type
    Collection
    ...Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Our Packaged on Request service offers you...

  46. AAV for Neuronal Tracing

    Type
    Collection
    ...):639-47. PMID: 17329205 Don’t See What You’re Looking For? Our Packaged on Request service offers you...

  47. Institute for Protein Innovation

    Type
    Collection
    ...ligand-binding pocket Bind specific subunit pairs, blocking ligand binding and receptor function Figure 3:...

  48. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...IgG2a 220411 LRRK2/Dardarin kinase, C-terminus [N241A/58R] LRRK2/Dardarin kinase, C-terminus Human Mouse ...several protocols available that may be helpful for working with R-mAbs expressed from the plasmids in this...

  50. Viral Vector Guides and Plasmids

    Type
    Collection
    ... expression. Retroviral vectors are popular for making stable cell lines or clinical use. Adenoviral Vector...
Showing: 51 - 100 of 933 results