...use Twitter professionally to network, learn more about alternative careers for scientists, and share cool....” This paper addresses the following questions about scientists on Twitter: who are they? What do they...

...the Addgene Blog How did this change in labor laws come about? In March 2014, the White House issued a...must be above this level, and certain job duty tests must be met. In the Department of Labor’s general... to $47,476 per year, under updates to the Fair Labor Standards Act (FLSA). This is having a major effect...ensuring that either all affected scientists are paid above this threshold or for tracking the hours that these... a memorandum to the Secretary of Labor to update the FLSA, which had not seen revisions since 2004. In... In July 2015, the Department of Labor issued a Notice of Proposed Rulemaking, soliciting feedback by ...adjusted every 3 years. In May 2016, Secretary of Labor Thomas Perez updated the overtime regulations of...

... she spoke with Teytelman about an opportunity to work with a collaborative group, she also joined early...guide on Transferable Skills: External Collaborations Learn about the 2018 Minisymposium on Scientific ...organizations with similar ideas who were also thinking about reproducibility and shared similar desires to take...individuals with similar ideas to actually do something about it. Building a diverse team with different talents...the eLife ambassadors group was already thinking about ways to tackle irreproducibility. Benjamin Schwessinger...grassroots efforts. After thoughtful conversations about reproducibility with Teytelman, Schwessinger joined...previous work at Center for Open Science and Sense about Science. However, she realized that she couldn’t...

...else to focus on. Use collaborative cloud apps It is a good idea to use collaborative cloud tools such as...creative collaboration in the workplace. There are many other ways to encourage collaborations remotely...meeting? No problem! You can still creatively collaborate and brainstorm! Image from Joseph Mucira. ...serve as a springboard for future discussion. Collaboration and creativity are pivotal in writing publications...Vicens et al., 2007) geared towards enhancing collaborative writing and this recent article published in...approaches/tools we have to foster creative collaboration remotely in science are endless. Let’s adapt...blog post “Fostering Communication in Remote Collaborations” to get more ideas. Thank you to our guest...

...successes in metabolic engineering have been limited to the overexpression of various metabolites in Escherichia...species or metabolic pathways. A new study takes us one step closer to the vision of metabolic biofactories...cellular behaviors, such as differentiation or metabolism. Our attempts to manipulate these cellular behaviors...be host-independent, beyond the oft published metabolic engineering realm of S. cerevisiae and E. coli...control via scRNA design is readily apparent in metabolic engineering. The authors elegantly demonstrated...demonstrated the potential of this to control a metabolic network in S. cerevisiae by using three RNA programs ...programs, the authors sequentially rerouted the metabolic flux to generate differently pigmented derivatives...

... And what is ligase fidelity anyway? Let’s talk about it. DNA ligases are enzymes that seal breaks in ...to accelerate characterization of nucleic acid metabolic enzymes." Nucleic acids research 44.2 (2015): ...

...our blog post about remote collaborations Read our Transferable Skills Guide article about external collaborations...needed. In the example above, for example, we fostered a formal scientific collaboration between two research...project. We think that these collaborations add enormous value. A discussion about a protocol can save weeks..., the researchers did not have access to a P3 laboratory (BSL-3), a high-security facility needed to perform...approach was useful in humans. Then, one of them heard about our online platform, called Crowdfight, and filed...just the right infrastructure and offered to collaborate with them. The partnership was fruitful and a...This example clearly illustrates the power of collaboration, which despite the competitiveness of cutting-edge...

...particles that are suitable for in vivo use. Learn more about our viral service ID Viral Prep Description PI 65417...well as by our trusted partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity...

...Connie Cepko to learn about her mentoring style. Top 7 questions scientists ask me about mentoring In this... answers to the top questions scientists ask me about mentoring. Here they are: What makes a good Mentor... Where can I find a Mentor? What is this I hear about “group” or “peer” mentoring? How do I ask someone...a good science mentor? I started a conversation about many of these topics with the researchers who joined...often one that devotes time and energy to thinking about how to be a great Mentor.    Check Out Angela Depace's...questions in future posts. If you have a question about mentoring that you want us to cover, let us know...

...new conference rooms and learned some fun facts about these genes and the origins of their names!  ...

...), Antibodies: a Laboratory Manual, 2nd Edition. 2104. Cold Spring Harbor Laboratory Publications, New...production and subsequent purification.  Academic laboratories and companies have already begun creating plasmids...Introduction to Antibodies blog post Learn more about hybridomas Watch our Antibodies 101: What is an ...

...well as by our trusted partner labs. To learn more about antibodies and their use, visit our Antibody Guide...

...Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling...

...Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling...

...well as by our trusted partner labs. To learn more about antibodies and their use, visit our Antibody Guide...

...Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling...

...proteins for distribution to academic and nonprofit laboratories for research and educational use. Links to additional...

...Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling...

...believes that creating and maintaining a highly collaborative NET research community is pivotal in accelerating...

...cloned for expression as TALENs. Additional details about the REAL, REAL-Fast, and FLASH assembly methods ...

...TALEN expression vectors. More detailed information about REAL, including detailed and up-to-date protocols...

... point for multiple biochemical signals. In collaboration with numerous research labs from around the ...

...their DNA design and synthesis capabilities, in collaboration with researchers at Stanford University’s Department...

...construct gRNA expression vectors. More information about RGNs and CRISPR-Cas systems can also be found at...

...it will be listed on the Function section pages above. You can also do a search of viral preps and filter...

..., Fabs, and secondary antibodies. To learn more about antibodies and their use, visit our Antibody Guide...

...Learn more about chemogenetic resources available at Addgene. See our inventory of chemogenetic constructs...

...'s Optogenetics Guide . Interested in more news about optogenetics research? Find related posts on Addgene's...

...Grade DNA is an Addgene Beta service. Learn more about Addgene's Beta Projects and how you can provide ...

...Representation of RESOLUTE mission. The combination of metabolomics and genome engineering forms the core of the...

...Dove et al., Nature 1997 ) and later modified by Pabo and colleagues ( Joung et al., PNAS 2000 ). The ...

... our Cre-lox Plasmids page for more information about these molecular tools. Cre AAV ID Name Promoter ...

...available Non-toxic to living cells To learn more about this system, visit the New England Biolabs website...

... Chemogenetics Guide pages for more information about this field. Receptor hM3D(Gq) DREADD rM3D(Gs) DREADD...

...Szutl, et al 2019 PMID: 31084567 . More information about ARF Superfamily members can be found in: ARF GTPases...

...individual plasmids is a bacterial stab (of a laboratory E. coli strain). Addgene continues to monitor...

... approach has also been developed by the Fire laboratory and described in Efficient Marker-Free Recovery...

...Additional Resources Viral Vector Production Learn about how we produce high-quality viral vector preps at...

...researchers who are learning to wield them. Learn more about Addgene’s TAL effector and TALEN kits here . References...

...are reported with each shipment. For more details about our viral vector packaging service, visit our Viral...

...constructed using the cpFRET kit from the Pertz laboratory. Empty Vectors Encoding Fluorescent Proteins ...

...engineering and directed evolution. More information about the collection can be obtained at the Open Bioeconomy...

...Information The plasmid below is available in each of the above serotypes and buffers. Please click on the plasmid...

...will aid neuroscientists in studying circuits in laboratory animals and human tissue specimens. These technologies...

...This library is anticipated to be useful for laboratories performing phosphoproteomic experiments in need...

...myristoylation signal For more information about tags, including amino acid sequences, see our...

... or shRNA; see article for detailed information about cloning and additional plasmids tetR-KRAB On Aebischer...

.... Read our CRISPR 101 post for more information about HDR/repair template design. How to use CRISPR to...

...and Optogenetics Guide page for more information about this field. Use the table and filters below to find...

...Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the Zhang Lab CRISPRs Browse All Zhang Lab Plasmids...