Skip to main content
Addgene
Showing: 51 - 100 of 627 results

  1. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ... Enzymes: SpeI , NotI , PstI Scar: TACTAGAG or TACTAG Features: 8 bp Scar; No In-Frame Fusions; Coding...Suffix Enzymes: Nhel , NotI , PstI Scar: GCTAGT Features: 6 bp scar encodes Ala-Ser; Allows for in-frame... CTCGAG Suffix Enzymes: BamHI , Xhol Scar: GGATCT Features: Scar encodes Gly-Ser in-frame with prefix ..., PstI Scar: ACTAGA Features: Modified RFC 10 with shortened prefix/suffix giving in-frame scar encoding... CTGCAG Suffix Enzymes: Agel , Spel , Notl, Pstl Scar: ACCGGC Features: Modified RFC 10 w/ start and stop...

  2. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Cre Yes 137135 pAAV-Ef1a-oScarlet None Yes 137136 pAAV-Ef1a-Con/Fon-oScarlet Cre AND Flp Yes 137137 pAAV-Ef1a-Con...pAAV-Ef1a-Con/Foff 2.0-oScarlet Cre AND NOT Flp FRT/F5 Yes 137138 pAAV-Ef1a-Coff/Fon-oScarlet Flp AND NOT Cre...137158 pAAV-nEF-ChRmine-mScarlet None Yes 137159 pAAV-nEF-Con/Fon-ChRmine-oScarlet Cre AND Flp Yes 137160...Fon-ChRmine-oScarlet Cre AND NOT Flp FRT/F5 Yes 137161 pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet Flp AND NOT...PubMed (Link opens in a new window) Poulin JF, Caronia G, Hofer C, Cui Q, Helm B, Ramakrishnan C, Chan...LE, Ramakrishnan C, Silberberg G, Deisseroth K, Carlén M, Meletis K. 2019. A hypothalamus-habenula circuit...

  3. CRISPR Guide

    Type
    Collection
    ...CRISPR plasmid will have all desired genomic edits carried out by Cas9. Multiplexing applications include ... and delivered to specific genomic locations to carry out unique tasks. We’ll discuss more Cas9 fusion...location in the genome, where the transposase then carries out integration of donor DNA. Both types of CASTs...with the sequence, so the edit is not considered “scarless”. Figure 7: Overview of CRISPR transposases Browse...that displays improved specificity, and editors carrying an ADAR truncation that retain RNA editing capabilities... Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013)...Jaenisch, R. (2013). One-Step generation of mice carrying mutations in multiple genes by CRISPR/CAS-Mediated...

  4. NETRF

    Type
    Collection
    ...discover cures and more effective treatments for carcinoid, pancreatic, and related neuroendocrine cancers... Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou Harvard University Epigenetic... Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Bradley Bernstein Broad Institute Identifying...Altered Epigenetic States and Drivers in Intestinal Carcinoid and Pancreatic Neuroendocrine Tumors...

  5. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Red calcium sensor PinkyCaMP based on mScarlet PinkyCaMP a mScarlet-based calcium sensor with exceptional... the sensor's behavior in the target system. Use care when applying a biosensor in a novel context or ...proteins enable metabolic assays at the point of care. Science. 2018 Sep 14;361(6407):1122-1126. Kai Johnsson...-regulatory role of the mitochondrial pyruvate carrier MPC. eLife. 2020 Mar 6;9. pii: 53917. Luis Felipe...sensor modules Grashoff Lab Tension Sensor Plasmids Carsten Grashoff Tension tCRMod tension sensors Tunable... voltage sensor to detect neural activity (Ace-mScarlet) A high-speed, bright, red fluorescent voltage...

  6. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ... expert viral vector production team carefully review your cargo/serotype selection after you submit your...′ ITR exceeding 4.7 kb Transfer plasmids with a cargo that may reduce titer due to toxicity or other reasons...Disclaimer Due to the novel nature of experiments carried out with our materials, Addgene cannot guarantee...

  7. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...118744 pScarlet-Kif5a KIF5A mScarlet CMV ALS Beverly Koller 118745 pCAG-Scarlet-Kif5a KIF5A mScarlet CAG ...mKate2 hsp70 ALS Caren Norden 105971 pME mKate2-dynactin1-811 DCTN1 mKate2 ALS Caren Norden 106093 pFRT_TO_FlagHA_TIA1...Arrowsmith 184825 pSN837 KIF5A mScarlet CMV ALS Kyoko Chiba 184826 pSN838 KIF5A mScarlet CMV ALS Kyoko Chiba 184827...184827 pSN526 KIF5A mScarlet Strep polH ALS Kyoko Chiba 184832 pSN840 KIF5A mScarlet Strep polH ALS Kyoko...JCE692) PRKCG PKC1 Spinocerebellar ataxia 16 Juan Carlos Igual 89925 Profiline PFN1 T7 ALS Guillaume Romet-Lemonne...Marius Wernig 176852 UBQLN2_450-624W UBQLN2 T7 ALS Carlos Castañeda 176915 pcDNA3.1(+)_Paragranulin+linker...TMEM106B Halo Frontotemporal dementia in GRN mutation carriers Michael Ward 178174 VAPB_Halo_C_allele VAPB Halo...

  8. Luciferase Plasmid Collection

    Type
    Collection
    ...experimental systems. LumiLuc and LumiScarlet : LumiLuc and LumiScarlet are ATP-independent luciferases ...Insertion of imperfect miRNA sequence in the 3' UTR Carl Novina 33154 pAC-Luc Firefly Creation of N-terminal...regions in Drosopholia Herman Wijnen 126033 pTETRIS-cargo Firefly For use as a reporter of the ability of ...fusion for expression in plants, co-expressed with mScarlet-I for ratiometric imaging David Nelson 141301 ...

  9. Plasmids for Stem Cell Research

    Type
    Collection
    ...Species Article PI iPSCs Cardiomyocytes Lentiviral Human Robust cardiomyocyte differentiation from human...reprogramming to cardiomyocytes using calcium activity as a functional measure of success. Mol Cell Cardiol. 2013...Sci U S A. 2012 May 29. Palecek Fibroblasts Cardiomyocytes Lentiviral Mouse Optimization of direct fibroblast...

  10. Immunology Research Plasmids and Resources

    Type
    Collection
    ...nuclear receptor subfamily 1, group I, member 3 CAR, CAR1, MB67, MGC150433, MGC97144, MGC97209 NR2C1 nuclear...MGC126262, PSCTK1, XLA CARD11 caspase recruitment domain family, member 11 BIMP3, CARMA1, MGC133069 CD19 CD19... TDGF1 teratocarcinoma-derived growth factor 1 CR, CRGF, CRIPTO, Cripto-1 TDGF3 teratocarcinoma-derived...2 BCL10 B-cell CLL/lymphoma 10 CARMEN, CIPER, CLAP, c-E10, mE10 CARD11 caspase recruitment domain family...ECD, SEM1, SHFD1, SHSF1, Shfdg1 SLC10A2 solute carrier family 10 (sodium/bile acid cotransporter family..., RAC-gamma, STK-2 BCL10 B-cell CLL/lymphoma 10 CARMEN, CIPER, CLAP, c-E10, mE10 BLNK B-cell linker BASH..., CKLF2, CKLF3, CKLF4, HSPC224, UCK-1 CLCF1 cardiotrophin-like cytokine factor 1 BSF3, CISS2, CLC, NNT1...

  11. The Pleiades Promoter Project

    Type
    Collection
    ...intron-lacZ/NLS Ple18 CARTPT pEMS1243 EGFP/NLS Ple20 CARTPT pEMS1245 EGFP/NLS Ple21 CARTPT pEMS1246 EGFP/NLS...NLS Ple21 CARTPT pEMS1492 intron-lacZ/NLS Ple22 CCKBR pEMS1493 intron-lacZ/NLS Ple23 CCKBR pEMS1494 intron-lacZ...

  12. Zhang Lab CRISPR Page

    Type
    Collection
    ...oligos (design is indicated below) can be cloned scarlessly into the vector before the sgRNA scaffold. The..., and a pair of annealed oligos can be cloned scarlessly into the vector before the sgRNA scaffold. The...top three significantly mutated genes in lung adenocarcinoma. Delivery of a single AAV vector ( AAV-KPL ...mutations, leading to macroscopic tumors of adenocarcinoma pathology. These plasmids as well as a backbone...

  13. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...pAAV-AiE2051m-minBG-SYFP2-WPRE3-BGHpA AiP1277 AiE2051m SYFP2 Layer 6_IT_Car3 Isocortex 220711 pAAV-AiE2051m-minBG-iCre(R297T...R297T)-BGHpA AiP1786 AiE2051m Cre Layer 6_IT_Car3 Isocortex 230518 pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA AiP1211... AiP1211 AiE2051m FlpO Layer 6_IT_Car3 Isocortex 224000 pAAV-AiE0772m-minBG-SYFP2-WPRE3-BGHpA AiP12612...AiP12612 AiE0772m SYFP2 Layer 6_IT_Car3 Isocortex 214570 pAAV-AiE2575m-minBG-SYFP2-WPRE3-BGHpA AiP1888 AiE2575m...

  14. Cre-lox system

    Type
    Collection
    ...31132 pCSCre Cre CMV Xenopus Ryffel 31309 pCARCre Cre cardiac actin Xenopus Ryffel 32144 pJFRC170-3XUAS-IVS-Cre...inducible - rrTA expression driven by mouse Nkx cardiac enhancer and promoter fragment minimal TetO-CMV...69916 pAAV.cTNT.iCre iCre and tdtomato Chicken cardiac troponin T AAV Pu 70120 pEMS2159 iCre with MCS ...Eisenhoffer 132551 AAV-U6-gRNA-TnT-Cre Cre Chicken cardiac troponin T AAV Pu 134308 pCAG-Synaptophysin-TdTomato-IRES2...

  15. Resolute Plasmid Collection

    Type
    Collection
    ...reagents to boost the research focused on Solute carrier proteins (SLCs). Browse the SLC plasmid collection...Collection Plasmids Pooled Libraries Feedback Solute carrier proteins (SLCs) are the largest family of transporters...experiments. These libraries target the solute carrier family of proteins. Library ID PI Description Human...

  16. Lentiviral Prep Service

    Type
    Collection
    ...Library Version 1 Ready-to-use lentiviral particles carrying version 1 of the CellTag barcoding library. Version...Library Version 2 Ready-to-use lentiviral particles carrying version 2 of the CellTag barcoding library. Version...Library Version 3 Ready-to-use lentiviral particles carrying version 3 of the CellTag barcoding library. Version...

  17. Brain Initiative Collection

    Type
    Collection
    ...pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE Expresses optimized PdCO in frame with mScarlet under control of...

  19. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...levels. This collection comprises 20 plasmids, each carrying two CRISPR guides. The first one binds to sfgfp... with CRISPR repeat motifs (squares), and also carrying the tracrRNA sequence (not shown). (B) Relative...

  20. Antibody Plasmid Collection

    Type
    Collection
    ...Companion to Methods in Enzymology 8, 94–103 (1995). Carlos Barbas Variant of the pComb3 phage display vector...J Immunol Methods. 2000 Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression, dimerisation...

  21. Plant Plasmids and Resources

    Type
    Collection
    ...species. In addition to individual plasmids, Addgene carries several useful toolkits and plasmid collections...microRNA and siRNA vectors in Arabidopsis - James Carrington. JoinTRV , an improved vector system based on...

  22. Zebrafish Plasmid Collection

    Type
    Collection
    ...days), short generation time (~3 months), ease of care and breeding, and transparency of the embryo and...destination vectors (pGCS), bearing either amino- or carboxyl-terminal tags, including Myc, HA, Flag, GST and...

  23. Viral Production

    Type
    Collection
    ...line is maintained for ~20 passages before being discarded and replaced with a new vial of early passage ...produced in the line will be taken off-line and discarded. Confirmation of Transfer Plasmid Addgene uses...

  24. TALEN Plasmids and Kits

    Type
    Collection
    ...Gate TALEN 2.0 38142 RCIscript-GoldyTALEN Daniel Carlson The plasmids pC-GoldyTALEN and RCIsricpt-GoldyTALEN..., and G) and the full length human LSD1 at the carboxy-terminus of the protein. 49043 EMM67 49044 EMM71T...

  26. Plan Your Experiment

    Type
    Collection
    ...sure to check whether reagents are available to carry out your experiment in your particular model organism...save your lab valuable time and resources when carrying out CRISPR experiments. Read more about how to...

  27. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...site. Design additional primers to analyze for scarring (small indels produced at the sgRNA cleavage site...to amplify the sgRNA target site to examine for scarring. This may be useful to characterize the non-deleted...

  28. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...CRISPR/Cas9-assisted Removal of Mitochondrial DNA (CARM) Library 82480 Knockout Mouse Xie N/A N/A 395 CRISPRa...Knockout Human Draetta 3rd 3-4 17,032 arrays Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library...

  29. Genetic Code Expansion

    Type
    Collection
    ...126035 pEVOL-ABK PylRS M. barkeri 3’-azibutyl-N-carbamoyl-lysine (AbK) Bacterial TAG Andrea Musacchio 127411...pRSF-ChPylTMSK ChPylTMSK N6-(((Trimethylsilyl)methoxy)carbonyl)-L-lysine (TMSK) Bacterial TAG Thomas Huber 163916...

  31. KLF Research Plasmids

    Type
    Collection
    ... metabolism, fibrosis, atherosclerosis, and carcinogenesis. The defining feature of the KLF family is ...

  32. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...ADAR2 by destabilizing its RNA binding. Editors carrying the delta-984-1090 ADAR2 truncation retain RNA...

  36. Plasmid Collections

    Type
    Collection
    ...Resource RESOLUTE Plasmid Collection for Solute Carrier Proteins (SLCs) Structural Genomics Consortium ...

  37. Chemogenetics Plasmids

    Type
    Collection
    ...(Gq), rM3D(Gs), and hM4D(Gi) are all based on muscarinic receptors and couple to the Gi, Gq, or Gs G-proteins...

  39. Mammalian RNAi Tools

    Type
    Collection
    ...pSicoligomaker (Andrea Ventura lab) References Carthew, R. W., & Sontheimer, E. J. (2009). Origins and...

  41. Recombinases AAV Preps

    Type
    Collection
    ... 196410 AAV-GfaABC1D-Cre-4x6T GfaABC1D none 5 Carmichael 107788 AAV.rTH.PI.Cre.SV40 rTH none 9, rg* Wilson...

  42. Synthetic Biology - Overview

    Type
    Collection
    ...Anderson Adam Arkin Gabor Balazsi Matthew Bennett Carolyn Bertozzi Nicolas Buchler George Church Cynthia ...

  43. Antibody Production

    Type
    Collection
    ...Actual shipping conditions will be shown in your cart. Need Some Help? Browse some of our other resources...

  45. Distribution to Industry

    Type
    Collection
    ...from the Structural Genomics Consortium Solute Carrier Proteins (SLCs) from the RESOLUTE collection AAV...

  46. AAVED

    Type
    Collection
    ...Materials don't have to be organized (yet), we will take care of that. Note that you will have final approval ...

  47. Microbiology Resources

    Type
    Collection
    ...Saccharomyces Cerevisiae Archive for Functional Analysis (EUROSCARF) (Link opens in a new window) JGI Integrated Microbial...

  48. Brain Armamentarium

    Type
    Collection
    ...Description PI Viral Preps ID Viral Prep Description PI (Cargo) PI (Capsid) 214869-PHPeB AiP15140: pAAV-AiE0873m...

  49. Ras Pathway

    Type
    Collection
    ...receptor bound protein 2 ICMT Isoprenylcysteine carboxyl methyltransferase INSR Insulin receptor INSRR ...
Showing: 51 - 100 of 627 results