...retrograde neuronal tracing. Note : Addgene does not distribute rabies virus. AAV Vectors for Monosynaptic Neuronal..., Young JA, Callaway EM. 2007b. Monosynaptic restriction of transsynaptic tracing from single, genetically...

...Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant antibodies made from...neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant mAb Collection . Once the plasmids...

...mTORC1 integrates these signals to determine the nutritional state of the cell and regulates the switch from...lysosome biogenesis. mTORC2 does not respond to nutrients, but it is activated by growth factors, including...

...successfully adopt these tools. Thank you for your contributions and your commitment to open science! Please ...meeting preparation section for how to best help contribute to this publication. Citation information for...

...Shortly after though, three groups independently tried reproducing these results and determined that even... expression. References: Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos M, Gibbs D, Kim SR,...

...heard stories and read papers wherein a researcher tries 5 separate experiments at once to get confirmatory...procedures like pouring a plate or running a gel have intricacies that aren’t always mentioned in static written...

...GTPase, and GAP set is referred to as a minimal triad, with each component involved in GTPase signaling..., Gateway GTPase Sar1b 51128 198 Gateway GTPase Trim23 373 574 Browse ARF GAP and GEF Plasmids by Gene...

...zebrafish ( Danio rerio ), named for the horizontal stripes on the sides of the adult body, is a tropical freshwater...Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish lines from European researchers...

... Kit comprised of 834 plasmids of pre-assembled trimer and tetramer RVD domains designed for rapid TALEN...cell lines. Features include (i) Esp3I (BsmBI) restriction sites for full compatibility with the Golden ...

...caused by abnormal expansion of a repeating CAG triplet series within the huntingtin (HTT) gene. An individual... the disease, a small portion of cases can be attributed to known genetic factors such as defects in LRRK2...

...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations...Goldstein Nematode: Injection and selection for Cas9-triggered homologous recombination Cas9-sgRNA target construct...

...TCTTCTTGTGCTCTTATTggcaggtacctggtgacTCACCTTATGGGCGCTGA matrixmetallopeptidase15 (membrane-inserted) TAL3484 & TAL3485 ...TGATCTGTTCCTGGTAGCcgttcatgagctgggcCATGCCCTTGGACTGGAA matrixmetalloproteinase14a (membrane-inserted) TAL3486 & TAL3487...

...Constitutive 9 Patriarchi 187180 pAAV_hSyn1_nLightR Syn nLightR none Constitutive 9 Patriarchi Oxytocin (OT...

...Vector Core will produce viral-based vectors for distribution via Addgene. For a full list of Addgene's viral... The following vectors are now available for distribution at Addgene: Penn ID Addgene ID Name Function...

...engineering and expressing zinc finger proteins for distribution to academic and nonprofit laboratories for research...

... iNDI iPSC line generation, phenotyping, and distribution efforts. Plasmids The following plasmids can...

... Antibodies Addgene distributes ready-to-use recombinant antibodies to help facilitate...

... plasmids containing KLF, we encourage you to contribute to this community resource. Learn about the benefits...

...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations...

...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations...

...is an encyclopedia of CRISPR systems with wiki entries describing enzyme activity, experimental considerations...

...Collections Pooled Libraries Antibodies NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection CRISPR CRISPR-on...

...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations...

...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations...

...is an encyclopedia of CRISPR systems with wiki entries describing enzyme activity, experimental considerations...

...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations...

... and place them in the public domain without restriction. Many SGC plasmids from the Toronto, Oxford and...

... All Antibodies All Antibodies Addgene distributes ready-to-use recombinant antibodies. These monoclonal...

...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, PAM requirements, experimental...

...into the pU6-BbsI-chiRNA plasmid via the BbsI restriction sites. Design oligos based on the following template...

...their Class 2 counterparts like Cas9. This is attributed in part to the difficulties in getting all components...

...repeat arrays of any desired length: the REAL (Restriction Enzyme And Ligation) assembly method (Sander ...

...thermal stabilization, and changes of voltage all contribute to assess ligand action/engagement. Protein production...

...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations...

... (Link opens in a new window) develops protein-centric Course-based Undergraduate Research Experiences...

...encourage other groups to join our community and contribute their own plasmids in the future. AVEXIS: One...

...receptor to specific DNA sequences. Ligand binding triggers a conformational change in the receptor and results...

...this technology are available at Addgene for distribution to the research community. Key features: Allows...

...Center (CGC) - The CGC collects, maintains, and distributes stocks of C. elegans. Silencing Genomes - Cold...

...The ABRC collects, preserves, reproduces, and distributes diverse seed and other stocks of Arabidopsis ...

...preparation from the inventory below. Lentiviruses are distributed as purified viral particles in solution that ...

...most popular virus for gene transfer in clinical trials. These viruses have a relatively high transduction...

...pAAV-CBh-mKate2-IRES-MCS (WT-WT) CBh mKate2 Constitutive 2 Patrick 114469 pAAV-CaMKIIa-mCherry CaMKIIa mCherry Constitutive...

...filled depends on cellular factors. Multiple labs tried to identify beneficial factors to include in even...bases. Thus, base editors have an activity window restricted to the stretch of ssDNA created by Cas9 binding...

...There is something akin to a frenzy as the crowd tries to weigh and determine the value of every sweet ...This post was contributed by guest blogger Cameron MacPherson at the Institut Pasteur CRISPR software...falciparum (the parasite that causes malaria). I attribute their success to technical expertise, thoughtful...Cas-OFFinder provides a bare-bones data dump of ALL strings in a genome using any IUPAC encoded pattern at ...biotechnology(2014). PubMed PMID: 24880488. 2. Hsu, Patrick D., et al. "DNA targeting specificity of RNA-guided...

...subunit alpha PREX2 Phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 PRKA PRKAA1 ...

...expressing recombinant phosphoproteins. These attributes make rEcoli XpS ( 192872 ) and pSerOTS-C1* ( ...

...encode a wide range of gene products. The main restrictions on a lentiviral transfer plasmid are size (i.e...

...on and off systems. Dox also has good tissue distribution, low toxicity, a known half-life (24 hours),...

... is typically used in neuroscience to control electrical potentials in neurons. See our Optogenetics Plasmids...