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Showing: 1 - 20 of 200 results
  1. pCAGGs-NLS-TIR1_P2A_NES-TIR1

    Plasmid
    #117699
    Purpose
    Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA
    Depositor
    Inserts
    TRANSPORT INHIBITOR RESPONSE 1
    TRANSPORT INHIBITOR RESPONSE 1
    Use
    Tags
    HA tag, Myc-NES, and NLS
    Expression
    Mammalian
    Mutation
    codon optimized for murine expression
    Promoter
    None, 2A peptide from porcine teschovirus-1 polyp…
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. YIp204-PADH1-atTIR1-9myc

    Plasmid
    #99532
    Purpose
    S. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)
    Depositor
    Insert
    TIR1 (TIR1 Mustard Weed)
    Use
    Tags
    9xMyc
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
  3. pAN19-3xFLAG-ccvTIR1-NOSt

    Plasmid
    #108546
    Purpose
    Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator
    Depositor
    Insert
    TRANSPORT INHIBITOR RESPONSE 1 [F79G] fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
    Use
    Cloning vector
    Tags
    3xFLAG
    Expression
    Mutation
    Changed F79 to G
    Promoter
    Available since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pAN19-TIR1-GUS-NOSt

    Plasmid
    #108547
    Purpose
    Cloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator
    Depositor
    Insert
    TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)
    Use
    Cloning vector
    Tags
    GUS
    Expression
    Mutation
    Promoter
    Available since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. pAN19-ccvTIR1-GUS-NOSt

    Plasmid
    #108548
    Purpose
    Cloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator
    Depositor
    Insert
    TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)
    Use
    Cloning vector
    Tags
    GUS
    Expression
    Mutation
    Changed F79 to G
    Promoter
    Available since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. pTUB1:OsTIR1-3FLAG, SAG1:CAT

    Plasmid
    #87258
    Purpose
    TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.
    Depositor
    Inserts
    TIR1
    CAT
    Use
    Toxoplasma expression
    Tags
    3FLAG
    Expression
    Mutation
    Codon optimized for Toxoplasma gondii expression
    Promoter
    TgSAG1 361 bp and TgTUB1 2717 bp
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
  7. JE301: pMVP (L5-L4) Tir1-P2A-AID

    Plasmid
    #121722
    Purpose
    pMVP L5-L4 entry plasmid, contains Tir1-P2A-AID for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Tir1 linked by P2A to AID domain fused to gene of interest.
    Insert
    osTir1-P2A-AID (open)
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    9x myc (C-term on osTIR1)
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 200 results