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Showing: 361 - 400 of 617 results

  2. AAV-Syn-NS1

    Plasmid
    #175276
    Purpose
    AAV vector mediating bicistronic expression of NS1 gene of YFV-17D and dTomato with NLS
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    Nonstructural protein 1 (NS1) of YFV-17D; NLS-dTomato (POLY Yellow fever virus strain 17D)
    Use
    AAV
    Tags
    NLS-dTomato (P2A cleavage)
    Expression
    Mutation
    Promoter
    Synapsin
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    175276-plasmid-map-sequence-id-349086

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  3. K785R Smarca4-sfGFP

    Plasmid
    #107057
    Purpose
    Expression of dominant-negative K785R Smarca4 (Brg1) with C-terminal super-folder GFP fusion.
    Depositor
    Courtney Hodges, Jerry Crabtree
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    Super-folder GFP (sfGFP)
    Expression
    Mammalian
    Mutation
    K785R
    Promoter
    CAG
    Available since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107057-plasmid-map-sequence-id-201959

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  4. pAAV-DIO-NS1

    Plasmid
    #175273
    Purpose
    AAV vector mediating Cre-depenent expression of NS1 gene of YFV-17D
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    Nonstructural protein 1 (NS1) of YFV-17D (POLY Yellow fever virus strain 17D)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Synapsin
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    175273-plasmid-map-sequence-id-346765

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  8. AAV-TRE-NS1-dTomato

    Plasmid
    #175278
    Purpose
    AAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    NS1; dTomato (POLY Yellow fever virus strain 17D)
    Use
    AAV
    Tags
    dTomato (from bidirectional promoter)
    Expression
    Mutation
    Promoter
    bidirectional TRE promoter
    Available since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175278-plasmid-map-sequence-id-349702

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  12. K785R Smarca4-V5 IRES-puro

    Plasmid
    #107059
    Purpose
    Expression of dominant-negative K785R Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.
    Depositor
    Courtney Hodges, Jerry Crabtree
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    IRES-puroR and V5
    Expression
    Mammalian
    Mutation
    K785R
    Promoter
    CAG
    Available since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107059-plasmid-map-sequence-id-201961

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  19. pNSSCP

    Plasmid
    #183188
    Purpose
    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under T
    Depositor
    Hsiao-Chun Huang
    Article
    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1
    Inserts
    DivIVA and superfolder GFP fusion protein
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183188-plasmid-map-sequence-id-366663

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  34. pGEX2T-TEV CHMP1B 186-199 L188A/L192A

    Plasmid
    #108288
    Purpose
    Expresses residues 186-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-286
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 188 to A, changed L 192 to A
    Promoter
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108288-plasmid-map-sequence-id-245273

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  35. pGEX2T-TEV CHMP1B 4-199 L188A/L192A

    Plasmid
    #108289
    Purpose
    Expresses residues 4-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-284
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 188 to A, changed L 192 to A
    Promoter
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108289-plasmid-map-sequence-id-245274

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  36. pGEX2T-TEV CHMP1B 186-199 L192A/L195A

    Plasmid
    #108290
    Purpose
    Expresses residues 186-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-287
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 188 to A, changed L 192 to A
    Promoter
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108290-plasmid-map-sequence-id-245275

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  37. pGEX2T-TEV CHMP1B 4-199 L192A/L195A

    Plasmid
    #108291
    Purpose
    Expresses residues 4-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-285
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 192 to A, changed L 195 to A
    Promoter
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108291-plasmid-map-sequence-id-245276

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  38. ST-2xTstem(5A)-GFP

    Plasmid
    #162504
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One wildtype Tac stem region is inserted within ST and the other mutated Tac stem region is inserted between ST and GFP.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    GFP tagged ST6Gal1 mutant (ST6GAL1, IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    One Tac stem region is inserted within ST6Gal1 an…
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    162504-plasmid-map-sequence-id-323646

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  39. ST-2xTstem-GFP

    Plasmid
    #162503
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One Tac stem region is inserted within ST and the other Tac stem region is inserted between ST and GFP.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    GFP tagged ST6Gal1 mutant (ST6GAL1, IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    One Tac stem region is inserted within ST6Gal1 an…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162503-plasmid-map-sequence-id-323511

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  40. GFP-Cstem-Tac(5A)

    Plasmid
    #162497
    Purpose
    Expresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a is inserted between GFP and Tac(5A).
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    CD8a stem region and Tac full length with mutated stem region (IL2RA, CD8A Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five threonine residues are mutated into alan…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162497-plasmid-map-sequence-id-323484

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Showing: 361 - 400 of 617 results