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Plasmid#178106PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G8 + MTOR nick intron-45 sgRNAs
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA
Plasmid#173211PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
UseCRISPR; Prime editing (pe3)TagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2m
Plasmid#105066PurposeA yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in bacterial cellsDepositorInsertY-GECO2m
UseTags6x His tagExpressionBacterialMutationPromoterpBADAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-torZ
Plasmid#89956PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting torZ.DepositorInserttorZ gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2s
Plasmid#105067PurposeA yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cellsDepositorInsertY-GECO2s
UseTags6x His tagExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2.1s
Plasmid#105068PurposeA yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cellsDepositorInsertY-GECO2.1s
UseTags6x His tagExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Expression_vector RO1_1
Plasmid#158010PurposeExpression vector for the de novo designed protein Expression_vector_RO1_1DepositorInsertde novo designed protein RO1_1
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pU6-tevopreq1-GG-acceptor
Plasmid#174038PurposePrime editing in mammalian cellsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhU6Available sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-pegRNA-GG-acceptor
Plasmid#132777PurposePrime editing in mammalian cellsDepositorInsertExchangeable cassette
UseTagsExpressionMammalianMutationSee manuscriptPromoterU6Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_ORI vector
Plasmid#99296PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-Broccoli
Plasmid#124360PurposeOverexpression of circular RNA (Broccoli)DepositorInsertTornado-Broccoli
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-Tornado-nLuc
Plasmid#212611PurposeFor expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.DepositorInsertTornado-CVB3-nLuc
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Basic Vector
Plasmid#212936PurposeFirefly luciferase vector for investigating regions controlling transcriptionDepositorTypeEmpty backboneUseLuciferaseTagsExpressionMutationWTPromoterAvailable sinceJan. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL3 Promoter Vector
Plasmid#212939PurposeControl vector with constitutive firefly luciferase expressionDepositorInsertluc+
UseLuciferaseTagsExpressionMutationWTPromoterSV40Available sinceJan. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSNAP-tag (m) Vector
Plasmid#101135PurposeExpression of protein of interest with SNAP-Tag in Mammalian cellsDepositorTypeEmpty backboneUseTagsSNAP-tag (SNAP26m)ExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-Flag mTOR wt
Plasmid#26603PurposeMammalian expression of human flag-tagged mTorDepositorInsertmTOR (MTOR Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
Puro.Cre empty vector
Plasmid#17408DepositorTypeEmpty backboneUseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Control Vector
Plasmid#212937PurposeControl vector with constitutive firefly luciferase expressionDepositorInsertluc+
UseLuciferaseTagsExpressionMutationWTPromoterSV40Available sinceJan. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLJM1 Raptor GFP
Plasmid#112744Purposeexpress Raptor-GFPDepositorInsertRaptor-GFP (RPTOR Human)
UseLentiviralTagseGFPExpressionMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
myc-Rictor corrected
Plasmid#11367PurposeMammalian expression of myc tagged human RICTORDepositorInsertRictor (RICTOR Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceApril 27, 2006AvailabilityAcademic Institutions and Nonprofits only