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Mouse CRISPRi sgRNA library Dolomiti in backbone XPR_050, Set B

Pooled Library

#104091

Purpose

Crispri

Depositor

John Doench, David Root

Article

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Use

Lentiviral

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA3.2 mir-1-1 reporter mir193b-1

Plasmid

#46731

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir193b-1 (MIR193B Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir50-1

Plasmid

#46710

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir50-1

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir44-1

Plasmid

#46704

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir44-1

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir40-1

Plasmid

#46696

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir40-1

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir30-1

Plasmid

#46688

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir30-1 (MIR30A Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir28-1

Plasmid

#46684

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir28-1 (MIR28 Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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DH5alpha::DddI

Bacterial Strain

#179631

Purpose

For expression of toxic DddA

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Bacterial Resistance

Gentamicin

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only
Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A

Pooled Library

#92379

Purpose

CRISPRa

Depositor

John Doench, David Root

Article

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Has Service

Lentiviral Prep

Use

Lentiviral

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only
Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set B

Pooled Library

#92380

Purpose

CRISPRa

Depositor

John Doench, David Root

Article

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Use

Lentiviral

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only
Mouse CRISPRa sgRNA library Caprano in backbone XPR_502 (P65 HSF), Set A

Pooled Library

#92383

Purpose

CRISPRa

Depositor

John Doench, David Root

Article

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only
sgRNA pool for Streptococcus pneumoniae

Pooled Library

#170432

Purpose

Concise library targeting the whole genome of S. pneumoniae D39V with one sgRNA per operon or gene.

Depositor

Jan-Willem Veening

Article

Liu et al Cell Host Microbe. 2021 Jan 13;29(1):107

Expression

Bacterial

Use

CRISPR

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only
Mouse CRISPRa sgRNA library Caprano in backbone XPR_502 (P65 HSF), Set B

Pooled Library

#92384

Purpose

CRISPRa

Depositor

John Doench, David Root

Article

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only
Lenti-sgNkx2-1#1/Cre

Plasmid

#193221

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Nkx2-1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgNkx2-1#1 (Nkx2-1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter cel-mir-1

Plasmid

#46651

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-1

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir16-1 wt

Plasmid

#46683

Purpose

Expresses wild-type miR-16-1 in mammalian cells

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir16-1 wt (MIR16-1 Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pMALac-OMA-1(1-182)

Plasmid

#80350

Purpose

Expresses OMA-1 (1-182) in bacteria for protein purification purposes

Depositor

Sean Ryder

Article

Kaymak et al J Biol Chem. 2013 Oct 18;288(42):30463-7

Insert

OMA-1 (oma-1 Nematode)

Tags

6xHis-MBP-TEV

Expression

Bacterial

Mutation

Encodes AA residues 1-182

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pExpreS2-1

Plasmid

#175360

Purpose

ZeoR backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

Depositor

ExpreS2ion Biotechnologies

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Type

Empty backbone

Expression

Insect

Promoter

Fused Actin-HSP70

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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SREC 1: abu-1::gfp

Plasmid

#21824

Depositor

David Ron

Article

Urano et al J Cell Biol. 2002 Aug 19. 158(4):639-46.

Insert

abu-1 promoter (abu-1 Nematode)

Tags

GFP

Expression

Worm

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Rheb1 shRNA #1

Plasmid

#26625

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Rheb1 shRNA (RHEB Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only