Addgene

We narrowed to 10,181 results for: AGA

Showing: 3031 - 3060 of 10181 results

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pJJF144_SECGFP_sg2

Plasmid

#163865

Purpose

sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting GFP

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

R07E5.16 (U6)

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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Lyn-YFP-Clover-FRB1N

Plasmid

#162439

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

Lyn-YFP-Clover-FRB1N

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pJPS2957

Plasmid

#135591

Purpose

SNR6 has GAGAUUUAUUUC at position 96–107 deleted

Depositor

Jonathan Staley

Article

Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

Insert

snR6 (snR6 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

U6Δ12 has GAGAUUUAUUUC at position 96–107 deleted

Promoter

Native

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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PTG

Plasmid

#127198

Purpose

Single template U6 promoter minimal size plasmid

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Type

Empty backbone

Use

Empty sgrna plasmid

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pF3BGX-mTurquoise2

Plasmid

#138390

Purpose

RMCE vector for generating C-terminal mTurqouise2 fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

mTurquoise2

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-Sox3gRNA-T1

Plasmid

#127777

Purpose

Chicken sox3 gRNA target site 1, cloned into the pU6 sgRNA (backbone #92359)

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

sox3gRNAT1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pB-puro-mU6-RtcAshRNA1

Plasmid

#126613

Purpose

Expresses shRNA against human RtcA under mouse U6 promoter

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

RtcA shRNA-1

Use

RNAi

Tags

Expression

Mutation

Promoter

mouse U6 promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 SCAF8_isoD_CRISPR_resistant

Plasmid

#122468

Purpose

pDONR for Gateway cloning

Depositor

Jesper Svejstrup

Article

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF8 isoform D CRISPR resistant (SCAF8 Human)

Use

Gateway cloning

Tags

Expression

Mutation

Synonymous mutations conferring resistance to gRN…

Promoter

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 SCAF4_CRISPR resistant

Plasmid

#122465

Purpose

pDONR for Gateway cloning

Depositor

Jesper Svejstrup

Article

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF4 CRISPR resistant (SCAF4 Human)

Use

Gateway cloning

Tags

Expression

Mutation

Synonymous mutations conferring resistance to gRN…

Promoter

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pSM001

Plasmid

#122935

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML029W (Marked)

Use

Tags

Expression

Yeast

Mutation

YML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTA

Promoter

[tetO2]4inPgal1

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pSV_243

Plasmid

#122940

Purpose

Expression of the marked mRNA under the control of insertional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML029W (Marked)

Use

Tags

Expression

Yeast

Mutation

YML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTA

Promoter

Pyml029w-tetO(2)

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-STAT2

Plasmid

#117161

Purpose

Target site TTTAAGTTCCACAGACTTGGA

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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SEPT11 sh2 3m shRNA

Plasmid

#122024

Purpose

Negative control shRNA for SEPT11 sh2 shRNA (plasmid 122023).

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-Septin 11 isoform II

Plasmid

#120318

Purpose

Septin 11 isoform II expression (mCherry-tag at the N-terminus)

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pGLIS3.1.0-gDNA

Plasmid

#113780

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor GLIS3

Depositor

Kevin White

Insert

GLIS3 (GLIS3 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pTD3685

Plasmid

#113391

Purpose

alpha factor inducible Ty3

Depositor

Suzanne Sandmeyer

Article

Bilanchone et al PLoS Genet. 2015 Sep 30;11(9):e1005528.

Insert

Ty3

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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p204_LTJ_sgRNACD45.2_R2

Plasmid

#82673

Purpose

sgRNA targeting murine CD45.2 region 2. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting CD45.2 region 2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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B51

Plasmid

#74005

Purpose

gRNA for B108

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

gRNA

Use

CRISPR

Tags

none

Expression

Mutation

Promoter

U6

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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B53

Plasmid

#74006

Purpose

gRNA for B108

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

gRNA

Use

CRISPR

Tags

none

Expression

Mutation

Promoter

U6

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZFP1_iso1_2

Plasmid

#86361

Purpose

Encodes gRNA for 3' target of human ZFP1_iso1

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZFP1_iso1 (ZFP1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_MIXL1_1

Plasmid

#86335

Purpose

Encodes gRNA for 3' target of human MIXL1

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against MIXL1 (MIXL1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF580_2

Plasmid

#86332

Purpose

Encodes gRNA for 3' target of human ZNF580

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF580 (ZNF580 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-VR

Plasmid

#62286

Purpose

expression of VR sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

VR sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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MR015-PHOX2BLeftTALEN_NN

Plasmid

#49450

Purpose

Left TALEN for PHOX2B with NN

Depositor

Bruce Conklin

Article

Miyaoka et al Nat Methods. 2014 Mar;11(3):291-3. doi:

Insert

TALEN

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 30, 2013

Availability

Academic Institutions and Nonprofits only

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MR015-PHOX2BRightTALEN_NN

Plasmid

#49449

Purpose

Right TALEN for PHOX2B with NN

Depositor

Bruce Conklin

Article

Miyaoka et al Nat Methods. 2014 Mar;11(3):291-3. doi:

Insert

TALEN

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 30, 2013

Availability

Academic Institutions and Nonprofits only

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MR015-PHOX2BRightTALEN_NK

Plasmid

#49478

Purpose

Right TALEN for PHOX2B with NK

Depositor

Bruce Conklin

Article

Miyaoka et al Nat Methods. 2014 Mar;11(3):291-3. doi:

Insert

TALEN

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2013

Availability

Academic Institutions and Nonprofits only

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MR015-PHOX2BLeftTALEN_NK

Plasmid

#49477

Purpose

Left TALEN for PHOX2B with NK

Depositor

Bruce Conklin

Article

Miyaoka et al Nat Methods. 2014 Mar;11(3):291-3. doi:

Insert

TALEN

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-PM-BAF180-SH1

Plasmid

#41931

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BAF180

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
pmyc-GFP-TNRC6C-(928-1020)

Plasmid

#42026

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6C (TNRC6C Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa928-1020

Promoter

CMV

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-THAP11-A

Plasmid

#36993

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

THAP11 shRNA (THAP11 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2012

Availability

Academic Institutions and Nonprofits only