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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 19,682 results for: ACE

Showing: 4241 - 4280 of 19682 results

  3. pTU2S-a (colE1 origin)

    Plasmid
    #74091
    Purpose
    Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    None
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74091-plasmid-map-sequence-id-138318

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  5. pGen2.1-redCALWY-4

    Plasmid
    #50738
    Purpose
    Expresses redCALWY-4 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.
    Depositor
    Maarten Merkx
    Article
    Lindenburg et al ACS Chem Biol. 2013 Oct 18;8(10):2133-9.
    Insert
    gene for redCALWY-4 Zn2+ sensor
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50738-plasmid-map-sequence-id-76087

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  12. pCS4

    Plasmid
    #55751
    Purpose
    Produces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.
    Depositor
    Matthew Bennett
    Article
    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50
    Insert
    KanR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-59 of KanR
    Promoter
    T7-lac
    Available since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    55751-plasmid-map-sequence-id-86408

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  13. pBAD-tdTEVDB_C-15A/G4C

    Plasmid
    #177205
    Purpose
    Permuted td intron with evolved initiation sequence for the expression of circular mRNA
    Depositor
    Stephen Fried
    Article
    Lee et al ACS Cent Sci. 2021 Oct 27;7(10):1736-175
    Insert
    Permuted GFP with evolved initiation sequence for circular mRNA
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Permuted sfGFP after residue 52 & Changed Cyt…
    Promoter
    araBAD
    Available since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177205-plasmid-map-sequence-id-373215

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  16. pNOC-CRISPR

    Plasmid
    #100008
    Purpose
    Expresses Cas9-Nlux and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into genome
    Depositor
    Eva Farre
    Article
    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968
    Type
    Empty backbone
    Use
    Algae, nannochloropsis expression
    Tags
    Cas9-Nlux
    Expression
    Mutation
    Promoter
    Ribi promoter
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    100008-plasmid-map-sequence-id-201983

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  18. pGen2.1-redCALWY-1

    Plasmid
    #50739
    Purpose
    Expresses redCALWY-1 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.
    Depositor
    Maarten Merkx
    Article
    Lindenburg et al ACS Chem Biol. 2013 Oct 18;8(10):2133-9.
    Insert
    gene for redCALWY-1 Zn2+ sensor
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    50739-plasmid-map-sequence-id-76088

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  21. pAL1

    Plasmid
    #197285
    Purpose
    Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195
    Depositor
    Bogumil Karas
    Article
    Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.
    Insert
    Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197285-plasmid-map-sequence-id-389699

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  23. pZA-GFP-R5

    Plasmid
    #106256
    Purpose
    Constitutively expresses GFP fused with silaffin-R5 silica affinity peptide
    Depositor
    Urartu Seker
    Article
    Olmez et al ACS Omega. 2018 Jan 31;3(1):585-594. doi
    Insert
    Green fluorescent protein
    Use
    Tags
    Added silaffin R5 peptide to C terminus of GFP af…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    106256-plasmid-map-sequence-id-232729

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  26. pMB1-B-TelR

    Plasmid
    #204980
    Purpose
    Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains
    Depositor
    Veronica Godoy-Carter
    Article
    Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102
    Insert
    mRFP1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Added replications elements for Acinetobacter spe…
    Promoter
    Available since
    Sept. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    204980-plasmid-map-sequence-id-404861

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  30. BC-A1-002

    Plasmid
    #78689
    Purpose
    A strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.
    Depositor
    Brian Chow
    Article
    Magaraci et al ACS Synth Biol. 2016 Apr 25.
    Insert
    BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    78689-plasmid-map-sequence-id-171045

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  31. pVH016

    Plasmid
    #80400
    Purpose
    No anti-scaffold control with 3xFLAG tags for Western blots. Contains pBAD-CusR (response regulator) and pCusR-GFP(3xFLAG).
    Depositor
    Richard Murray
    Article
    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.
    Inserts
    ffGFP-3xFLAG
    CusR (cusR )
    AraC (araC )
    Use
    Synthetic Biology
    Tags
    C-terminal 3xFLAG tag and C-terminal LZx domain
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    80400-plasmid-map-sequence-id-155905

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  35. THI VP64 pGAP T-

    Plasmid
    #161487
    Purpose
    Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2
    Depositor
    Brigitte Gasser, Diethard Mattanovich
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2-VP64
    gRNA (Non targeting control NTC)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pGAP, pPOR1, and pTEF2
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161487-plasmid-map-sequence-id-326158

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  39. BC-A1-006

    Plasmid
    #78693
    Purpose
    A weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.
    Depositor
    Brian Chow
    Article
    Magaraci et al ACS Synth Biol. 2016 Apr 25.
    Insert
    BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    78693-plasmid-map-sequence-id-171252

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  40. dTomato_LD 0_CS G_tTA-BFP

    Plasmid
    #58871
    Purpose
    MESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58871-plasmid-map-sequence-id-94232

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Showing: 4241 - 4280 of 19682 results