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We narrowed to 18,142 results for: Met;

Showing: 4241 - 4280 of 18142 results

  10. deltaVP1d63C + VP2C-GFPdeg

    Plasmid
    #182481
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 with the 63 C-ter residues truncated (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-GFPdeg
    deltaVP1d63C
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182481-plasmid-map-sequence-id-360432

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  11. Linked VLP NES

    Plasmid
    #182432
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS-NES
    deltaVP1
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182432-plasmid-map-sequence-id-360114

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  12. Linked VLP NES (M)

    Plasmid
    #182434
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS(M)-NES
    deltaVP1
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182434-plasmid-map-sequence-id-360117

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  13. Linked VLP NES (G)

    Plasmid
    #182476
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96C)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS(G)-NES
    deltaVP1
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182476-plasmid-map-sequence-id-360123

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  14. pNHT7::UMSBP::sNluc2_ORF2

    Plasmid
    #186766
    Purpose
    An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at a start codon further downstream of the endogenous gene's start codon..
    Depositor
    Bo Wang
    Article
    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002
    Insert
    Nanoluciferase
    Use
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Oct. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186766-plasmid-map-sequence-id-375146

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  15. pCEP4_HLA-cmyc-GRM3 C127A T174A-VC

    Plasmid
    #140457
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and T174A mutant fused to the C-terminal half of split fluorescent Venus (VC)
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, Threonine 174 to alanine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140457-plasmid-map-sequence-id-292299

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  16. pPodA11

    Plasmid
    #162843
    Purpose
    Expression protein designed PodA10 from a TEV cleavable IPTG inducible plasmid with ampicillin resistance
    Depositor
    Dianne Newman
    Article
    VanDrisse et al Proc Natl Acad Sci U S A. 2021 Mar 23;11
    Insert
    Protein Designed Pyocyanin demethylase
    Use
    Tags
    TEV-cleavable 6x-His tag
    Expression
    Bacterial
    Mutation
    A53N, I73T, A87V, M99V, A129T from WT PodA
    Promoter
    T7
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    162843-plasmid-map-sequence-id-343491

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  17. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry

    Plasmid
    #69233
    Purpose
    R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)
    Depositor
    Scot Wolfe
    Article
    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…
    Expression
    Mammalian
    Mutation
    Arginine 1335 is changed to Lysine
    Promoter
    Available since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    69233-plasmid-map-sequence-id-122312

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  19. SL542

    Plasmid
    #49951
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD RH-8 (RHOXF2 Human)
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 22, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. SL544

    Plasmid
    #49970
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD RH-10 (RHOXF2 Human)
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  21. SL545

    Plasmid
    #49953
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD RH-11 (RHOXF2 Human)
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  22. SL543

    Plasmid
    #49952
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD RH-9 (RHOXF2 Human)
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  23. SL541

    Plasmid
    #49950
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD RH-7 (RHOXF2 Human)
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  24. SL540

    Plasmid
    #49949
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD RH-6 (RHOXF2 Human)
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  31. pR I-Ab beta Derp1-117

    Plasmid
    #113639
    Purpose
    Insect cell expression vector for I-Ab beta chain fused to Der p 1 117-127 antigenic peptide
    Depositor
    James Moon
    Article
    Moon et al Methods Mol Biol. 2018;1799:165-181. doi
    Insert
    I-Ab beta Derp1-117 (H2-Ab1 Mouse)
    Use
    Tags
    6x His tag, fused to Der p 1 (117-127) peptide vi…
    Expression
    Insect
    Mutation
    transmembrane and cytoplasmic domains truncated
    Promoter
    Metallothionein
    Available since
    Sept. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    113639-plasmid-map-sequence-id-221159

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Showing: 4241 - 4280 of 18142 results