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pAC84-pCR8-dCas9

Plasmid

#48218

Purpose

dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A)

Use

CRISPR; Gateway donor

Tags

HA Tag

Mutation

D10A;H840A

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pET14b-PITPalpha

Plasmid

#58256

Purpose

bacterial expression of human PITP alpha

Depositor

Shamshad Cockcroft

Article

Hara et al J Biol Chem. 1997 Jun 6;272(23):14908-13

Insert

PITP alpha (PITPNA Human)

Tags

HIS

Expression

Bacterial

Promoter

T7

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
Cathepsin S S21C/C25A

Plasmid

#84695

Purpose

for expression of mutant Cathepsin S in Leishmania tarantolae

Depositor

Hans Brandstetter

Article

Freier et al Sci Rep. 2015 Aug 3;5:12707. doi: 10.103

Insert

cathepsin S (CTSS Human)

Use

Expression in leishmania tarantolae

Tags

his-tag

Mutation

S21C, C25A

Promoter

unknown

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe puro H-Ras G12V Y40C

Plasmid

#12276

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V Y40C (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The Y40C mutat…

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
HA-FOXO3a TM delta CT

Plasmid

#1797

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

FOXO3a (FOXO3 Human)

Tags

HA

Expression

Mammalian

Mutation

T32A, S253A, S315A, delta CT (aa 468-673)

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pBabe puro H-Ras G12V E37G

Plasmid

#12275

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V E37G (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The E37G mutat…

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
pHS0667 KraIscB-1 non-coding locus in pCOLADuet-1

Plasmid

#176537

Purpose

Non-protein coding locus components from KraIscB-1

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

non-coding locus components from KraIscB-1 locus

Expression

Bacterial

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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HA-FOXO3a T32A DBM (H212R)

Plasmid

#1793

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

FOXO3a (FOXO3 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

T32A, S253A, S315A, H212R

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pUDE735

Plasmid

#103024

Purpose

expression of a Cpf1 programming crRNA targetting CAN1, HIS4, PDR12 and ADE2 (crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S)

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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GST-GAR

Plasmid

#34697

Depositor

Steven Clarke

Article

Frankel et al J Biol Chem. 2000 Oct 20;275(42):32974-8

Insert

Fibrillarin (FBL Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 1-145 of human fibrillarin with K2E and …

Promoter

tac

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5

Plasmid

#125236

Purpose

Expresses modified SPARK β2AR-NanoLuc transmembrane component in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5

Use

AAV

Tags

Flag, V5

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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Bxb1-ASAP2f

Plasmid

#183757

Purpose

Bxb1-specific donor plasmid for intergrating a transcription unit for expression of ASAP2f voltage reporter

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

ASAP2f

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pWZL Blast H-Ras G12V T35S

Plasmid

#12278

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V T35S (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The T35S mutat…

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
pBTK601

Plasmid

#110607

Purpose

BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Cas9

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pLuc-CDS-m215-2

Plasmid

#42102

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

ZEB1 (ZEB1 Human)

Use

Luciferase

Tags

Renilla luciferase

Expression

Mammalian

Mutation

Silent mutations were introduced in the seed-comp…

Promoter

SV40

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pMS6: pHelper(AvCAST)_entry_ΔTnsD

Plasmid

#168139

Purpose

Inducible expression of AvCAST proteins (except TnsD). Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL Blast H-Ras G12V E37G

Plasmid

#12277

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V E37G (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The E37G mutat…

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pWZL Blast H-Ras G12V Y40C

Plasmid

#12279

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V Y40C (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The Y40C mutat…

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
pEPQD1CB0107

Plasmid

#177040

Purpose

MoClo Level 1, position 1, transcriptional unit for transient expression of 1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pVENUS-N1-FNTA

Plasmid

#86859

Purpose

To express FNTA with a Venus tag at C terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

FNTA (FNTA Human)

Tags

Venus

Expression

Mammalian

Mutation

The sequence of the insert is isoform 2, which di…

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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Nanog-t2A-mCherry

Plasmid

#127541

Purpose

Expresses the transactivation domain of mouse Nanog and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

Nanog (Nanog Mouse)

Expression

Mammalian

Mutation

Codes for the last 110 a.a of Nanog

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1-pCR8-dCas9VP48

Plasmid

#48214

Purpose

dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP48 activation domain

Use

CRISPR; Gateway donor

Tags

HA Tag and VP48

Mutation

D10A;H840A

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pPD686 A2UCOE-EnAsCas12a-BFP

Plasmid

#249159

Purpose

Overexpression of EnAsCas12a-BFP with blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

A2UCOE-EnAsCas12a-TagBFP

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pSA127 A2UCOE-Cas9-BFP

Plasmid

#249151

Purpose

Overexpression of SpCas9-BFP and blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

A2UCOE-Cas9-TagBFP

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pUBC(k)-AT5G42080-mKOk

Plasmid

#224852

Purpose

Plasmid for expression of AT5G40280.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT5G40280.1 (ERA1 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCLJ-Fgf5-t2A-eGFP

Plasmid

#227504

Purpose

Inserts t2a-eGFP at the 3' end of the Fgf5 gene alsong with a removable selection cassette

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

EGFP

Use

AAV and Cre/Lox

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINF AtMKK5

Plasmid

#228396

Purpose

Recombinant expression of His6-tagged Arabidopsis thaliana MKK5 in E. coli

Depositor

Lennart Wirthmueller

Article

Simranjit et al bioRxiv 2023.09.25.558804

Insert

MKK5 (MKK5 Mustard Weed)

Tags

His6

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR4 HopU1

Plasmid

#228395

Purpose

pENTR4 plasmid with CDS of Pseudomonas syringae pv tomato type III effector HopU1 without stop codon for C-terminal epitope tags

Depositor

Lennart Wirthmueller

Article

Simranjit et al bioRxiv 2023.09.25.558804

Insert

HopU1

Use

Gateway-compatible entry vector

Mutation

Alanine inserted after start Methionine due to Nc…

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CamKII-mtPyronicSF

Plasmid

#228906

Purpose

Expression of mtPyronicSF in neurons targeted to the mitochondria serving as an optical sensor for mitochondrial pyruvate uptake. Backbone from Addgene plasmid 100834

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

Insert

pyronic SF

Use

AAV

Tags

Cox8 targetting

Expression

Mammalian

Promoter

CamKII

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV GFP

Plasmid

#213685

Purpose

Expresses Green Fluorescent Protein (GFP) with an N-terminal twin-Strep tag

Depositor

Thomas Kukar

Article

Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

Insert

Twin-Strep GFP

Use

AAV

Tags

Twin-Strep

Promoter

cytomegalovirus enhancer/chicken β-actin promoter

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Stargazin-EBFP2-FRB

Plasmid

#222417

Purpose

To express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

Cacng2 (Cacng2 Mouse)

Tags

EBFP2-HA-FRB

Expression

Mammalian

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET30b jGCaMP8f L27A

Plasmid

#204138

Purpose

Production of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coli

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f L27A

Tags

N-terminal His tag

Expression

Bacterial

Mutation

Leucine 27 changed to Alanine

Promoter

T7

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8f L27A

Plasmid

#204140

Purpose

Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with L27A mutation

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f L27A

Tags

N-terminal His tag

Expression

Mammalian

Mutation

Leucine 27 changed to Alanine

Promoter

CMV

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR4 genomic A. thaliana PLDGAMMA3

Plasmid

#209188

Purpose

pENTR4 plasmid with genomic sequence of Arabidopsis thaliana PHOSPHOLIPASE D GAMMA 3 (PLDGAMMA3, AT4G11840) without stop codon for C-terminal epitope tags

Depositor

Lennart Wirthmueller

Article

Simranjit et al bioRxiv 2023.09.25.558804

Insert

AT4G11840 (PLDGAMMA3 Mustard Weed)

Use

Gateway-compatible entry vector

Promoter

none

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG-GW1-GlcisPT

Plasmid

#203172

Purpose

Yeast expression vector for Giardia cis-prenyltransferase

Depositor

William Sessa

Article

Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

Insert

UPPS

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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Control V5-Twist

Plasmid

#201376

Purpose

Expresses Twist for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Tags

V5

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPIP1;4-NLS-3xmCitrine

Plasmid

#192524

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Article

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

PLASMA MEMBRANE INTRINSIC PROTEIN 1;4 (PIP1;4 Mustard Weed)

Tags

3xmCitrine and NLS

Expression

Plant

Promoter

PIP1;4

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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ptAD-seq-ubi63E-Gal4-DBD

Plasmid

#111930

Purpose

Vector to express tAD candidates from a library cloned from fragmented transcription factor coding sequences fused to the Gal4 DNA binding domain.

Depositor

Alexander Stark

Article

Arnold et al EMBO J. 2018 Aug 15;37(16). pii: embj.20

Insert

tAD-seq Gal4-DBD

Expression

Insect

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-ZNF354C_mutant_M41K-L42K-E43K

Plasmid

#194185

Purpose

Expresses ZNF354C with mutated KRAB domain (M41K, L42K, E43K mutation)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Oo et al Sci Rep. 2020 Nov 5;10(1):19079. doi: 10

Insert

ZNF354C (ZNF354C Human)

Tags

Myc-DDK

Expression

Mammalian

Mutation

KRAB domain mutant M41K/L42K/E43K

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pST_D4NWF2_DrHD_b03_dom3 (pBS0681)

Plasmid

#185207

Purpose

For the mammalian expression of the Deinococcus radiodurans protein D4NWF2_DrHD_b03_dom3. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

D4NWF2_DrHD_b03_dom3

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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