We narrowed to 35,837 results for: CaS

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RCAS DNhRARalpha (CC#1865)

Plasmid

#15153

Depositor

Connie Cepko

Article

Sen et al Development. 2005 Dec . 132(23):5147-59.

Insert

RAR alpha (dom neg) (RARA Human)

Use

Retroviral; Avian expression

Mutation

Dominant negative. Truncated at aa403, so lacks t…

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only
pAC1803_pmax-RBM38-dCasRx

Plasmid

#118637

Purpose

Transient transfection; Expresses RBM38-dCasRx; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

RBM38-dCasRx

Use

CRISPR

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMCSG7-D16A-NmeCas9

Plasmid

#71476

Purpose

bacterial pMCSG7 expression vector expressing Nme cas9 nickase with a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag and TEV site

Depositor

Erik Sontheimer

Article

Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

Insert

pMCSG7-D16A-NmeCas9

Tags

6His

Expression

Bacterial

Mutation

D16A

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-AkCas12b-2AeGFP

Plasmid

#121946

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AkCas12b

Tags

3xHA tag

Expression

Mammalian

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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LentiCas9-sgCh2-2-Blast

Plasmid

#199645

Purpose

Expresses Cas9 and sgRNA control guide targeting intergenic region

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Laccase2 Exons 1-3

Plasmid

#91799

Purpose

Drosophila laccase2 minigene plasmid that expresses both a linear RNA and a circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Mol Cell. 2017 Nov 9. pii: S1097-2765(17

Insert

Laccase2 (stw Fly)

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA

Plasmid

#112921

Purpose

Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

EF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo

Use

Gateway entry vector

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMCSG7-H588A-NmeCas9

Plasmid

#71477

Purpose

bacterial pMCSG7 expression vector expressing Nme cas9 nickase with H588A mutation (HNH domain) with T7 promoter, N-terminal His tag and TEV site

Depositor

Erik Sontheimer

Article

Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

Insert

pMCSG7-H588A-NmeCas9

Tags

6His

Expression

Bacterial

Mutation

H588A

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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RCAS GH6 (CC#152)

Plasmid

#15137

Depositor

Connie Cepko

Article

Schulte et al Development. 2000 Dec . 127(23):5033-45.

Insert

GH6 (HMX1 Chicken)

Use

Retroviral; Avian expression

Available Since

June 15, 2007

Availability

Academic Institutions and Nonprofits only
pLC-RFP657-CASP8

Plasmid

#75164

Purpose

LentiCRISPR-RFP657 with sgRNA targeting human Caspase-8

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

CASP8 sgRNA (CASP8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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PBLO CasX-gRNA

Plasmid

#126419

Purpose

E. coli vector for CasX gRNA --- Used for In Vitro Transcription

Depositor

Jennifer Doudna

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

casX gRNA

Use

CRISPR; In vitro transcription

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV_APOBEC1-UdgX-nCas9

Plasmid

#163531

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

APOBEC1-UdgX-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-ECT2_sgRNA

Plasmid

#183873

Purpose

pX459V2.0-HypaCas9 plasmid with ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ECT2 sgRNA spacer (ECT2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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BII-gR-BtW-eSpCas9

Plasmid

#133358

Purpose

Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

eSpCas9

Tags

Flag-tagged eSpCas9

Expression

Mammalian

Mutation

eSpCas9 mutations

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pRGEB-VirD2-Cas9

Plasmid

#139803

Purpose

Expressing VirD-Cas9 fusion under ubiquitin promoter

Depositor

Magdy Mahfouz

Article

Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

Insert

VirD2-Cas9

Tags

VirD2-Cas9

Expression

Plant

Promoter

Ubiquitin

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-Cas9-tdTomato

Plasmid

#141013

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Cas9

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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FnoCas12a-2C-NLS in pCSDest

Plasmid

#126639

Purpose

Expresses FnoCas12a-2C-NLS in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

FnoCas12a-2C-NLS

Use

CRISPR

Tags

3xHuman influenza hemagglutinin (HA)-Tag

Expression

Mammalian

Promoter

CMV IE94 promoter

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pRS416gT-GalL-Cas9

Plasmid

#79903

Purpose

pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression

Depositor

Ronald Davis

Article

Fu et al Nucleic Acids Res. 2016 May 19. pii: gkw

Inserts

Cas9
guide RNA (gRNA)
Tetracycline Repressor

Use

CRISPR

Expression

Yeast

Promoter

GPM1 promoter, GalL, and RPR1 promoter with TetO …

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-LmoCas8b2

Plasmid

#126482

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas8b2

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-LmoCas7

Plasmid

#126483

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas7

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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