We narrowed to 16,426 results for: Pol

1. pOpen-KTHEAQpol

   Plasmid

   #165542

   Purpose

   Exonuclease-free, N-terminal deletion mutant of Taq DNA polymerase.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   KlenTaq1 polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pOpen-THEAQpolA

   Plasmid

   #165512

   Purpose

   DNA polymerase used in routine PCR method because of its high thermostability, standard testing, screening and output testing.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   Taq DNA Polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pMAH-POLY

   Plasmid

   #64915

   Purpose

   Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

   Depositor

   Huiwang Ai

   Article

   Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-

   Inserts

   Polyspecific aminoacyl-tRNA Synthetase
   Amber Suppression tRNA
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6/H1

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. EP-Pol vector

   Plasmid

   #131686

   Purpose

   A lentiviral vector that constitutively expresses an error-prone variant of the adenovirus DNA polymerase

   Depositor

   Matthew D. Shoulders

   Article

   Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

   Insert

   Adenovirus DNA polymerase
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   F421Y, D827A

   Promoter

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOpen-9N7polA

   Plasmid

   #165500

   Purpose

   Thermophilic DNA polymerase; genetically engineered to have a decreased 3'-5' proofreading exonuclease activity

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   9 degrees N-7 DNA polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pOpen-SP6rpol

   Plasmid

   #165516

   Purpose

   DNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   SP6 RNA Polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pCas9-polyA

   Plasmid

   #72602

   Purpose

   express hCas9 byT7 promoter with polyadenine tails

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

   Insert

   Cas9-polyadenine
   

   Use

   Tags

   81-bp polyadenylation

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOpen-BstpolLF

   Plasmid

   #165553

   Purpose

   Fragment retains 5'-3' polymerase activity from full length Bst DNA Polymerase, while lacking 5'-3' exonuclease activity. Suitable for applications requiring thermophilic strand displacement.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   Bst DNA Polymerase, Large Fragment
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. T. aq RNA Polymerase

   Plasmid

   #215349

   Purpose

   Express T. aq RNA Polymerase subunits in E. coli cells

   Depositor

   Julius Lucks

   Article

   Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

   Insert

   RNA polymerase
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOpen-Pwopol

    Plasmid

    #165562

    Purpose

    DNA polymerase; additionally exhibits 3' to 5' exonuclease activity.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    Pwo DNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pOpen-Bstpol

    Plasmid

    #165543

    Purpose

    Full length DNA polymerase from Bacillus stearothermophilus.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    Bst DNA Polymerase, Full Length
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pOpen-TthpolA

    Plasmid

    #165533

    Purpose

    DNA Polymerase whose activity is resistant to prolonged incubations at high temperatures (95 degrees C).

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    Tth DNA polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pOpen-K12polLF

    Plasmid

    #165511

    Purpose

    DNA pol fragment that retains 5'-3' polymerase activity and 3'-5' exonuclease activity but loses 5'-3' exonuclease activity.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    DNA Polymerase I, Large (Klenow) Fragment
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pOpen-9N7polA (CT)

    Plasmid

    #165502

    Purpose

    9 Degrees North DNA polymerase variant with enhanced ability to incorporate modified substrates

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    9 degrees N-7 DNA Polymerase chain terminating
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. AE-Polyubiquitin-GCaMP6s

    Plasmid

    #106868

    Purpose

    Expresses polyubiquitin GCaMP6s, a genetically encoded calcium indicator, for live calcium imaging in Aedes aegypti mosquitoes.

    Depositor

    Omar Akbari

    Article

    Bui et al BMC Neurosci. 2019 Jun 17;20(1):27. doi:

    Insert

    AE-Polyubiquitin-GCAaMP6s
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti polyubiquitin (PUb)

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOpen-BsupolLF

    Plasmid

    #165563

    Purpose

    Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    Bsu DNA Polymerase I, Large Fragment
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pTol2CG NBT:NRGIIIa:polyA

    Plasmid

    #193012

    Purpose

    Tol2 transgenic construct that will express human Neuregulin1 Type IIIa in neurons

    Depositor

    William Talbot

    Article

    Lysko et al Hum Mutat. 2022 Sep;43(9):1216-1223. doi

    Inserts

    NBT
    NRG1 type IIIa (NRG1 Human)
    

    Use

    Transposon transgenesis

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. HDR-HaloPOLQ

    Plasmid

    #211637

    Purpose

    halo donor

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    Puro-LoxHaloTag
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1P POLE1_1

    Plasmid

    #160762

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPolII-Luc

    Plasmid

    #17223

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

    Insert

    PolII promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only