We narrowed to 7,570 results for: Pol

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pET28a-MSP1D1delltaH5

Plasmid

#71714

Purpose

Membrane scaffold protein (MSP), helix 5 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis and TEV site

Expression

Bacterial

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-UBF

Plasmid

#17656

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

UBF (UBTF Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 28, 2008

Availability

Academic Institutions and Nonprofits only

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pDONR223-PDGFRB

Plasmid

#23893

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDGFRB (PDGFRB Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pKMyc

Plasmid

#19400

Depositor

Ian Macara

Article

Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Type

Empty backbone

Tags

Myc

Expression

Mammalian

Available Since

April 2, 2009

Availability

Academic Institutions and Nonprofits only

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pmGFP-P2A-K0-P2A-RFP

Plasmid

#105686

Purpose

Double fluorescent stalling reporter K0

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K0
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pOpen_taq

Plasmid

#153315

Purpose

Bacterial expression of Taq polymerase

Depositor

Drew Endy

Insert

Taq polymerase

Expression

Bacterial

Promoter

Tac

Available Since

June 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-C1 beta-catenin WT

Plasmid

#16071

Depositor

Erin Schuman

Article

Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

Insert

beta-catenin (CTNNB1 Chicken)

Tags

EGFP

Expression

Mammalian

Mutation

*

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pDONR223-PDGFRA

Plasmid

#23892

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDGFRA (PDGFRA Human)

Use

Gateway Cloning

Mutation

M260I

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 TDP-43 YFP

Plasmid

#84911

Purpose

expression of TDP-43 YFP in mammalian cells

Depositor

Aaron Gitler

Article

Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_FL_WT

Plasmid

#98651

Purpose

Expresses MBP-tagged full length FUS

Depositor

Nicolas Fawzi

Article

Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBS hShh (CT#401)

Plasmid

#13996

Depositor

Cliff Tabin

Article

Marigo et al Genomics. 1995 Jul 1. 28(1):44-51.

Insert

Shh (SHH Human)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pJ4M/TDP-43

Plasmid

#104480

Purpose

bacterial expression of TDP-43

Depositor

Nicolas Fawzi

Article

Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

TDP-43 (TARDBP Human)

Tags

MBP-6xHis

Expression

Bacterial

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Full length Importin beta

Plasmid

#106941

Purpose

Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

Depositor

Patrizia Lavia

Article

Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

Insert

human Importin beta-1 (KPNB1) (KPNB1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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p23-puro-EGFP-RPA194

Plasmid

#179237

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

RPA194 (POLR1A Human)

Tags

EGFP

Expression

Mammalian

Promoter

EF-1alpha

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pET21b(+)-Is-PETase-W159H-S238F

Plasmid

#112203

Purpose

pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12

Depositor

Gregg Beckham, Christopher Johnson

Article

Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

Insert

PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12

Tags

6XHIS

Expression

Bacterial

Mutation

W159H, S238F

Promoter

N/A

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pC0040-LwaCas13a crRNA backbone

Plasmid

#103851

Purpose

Backbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAA

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

U6-LwaCas13aDR-BbsI-BbsI-polyT

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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FUW-SOK

Plasmid

#20327

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Sox2-F2A-Oct4-T2A-Klf4 (Sox2, Pou5f1, Klf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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Myc tag WT PTB

Plasmid

#23024

Depositor

Douglas Black

Article

Xie et al Proc Natl Acad Sci U S A. 2003 Jul 22. 1

Insert

PTB1 (PTBP1 Human)

Tags

Myc

Expression

Mammalian

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
pET28a-SnoopCatcher

Plasmid

#72322

Purpose

Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a-SnoopCatcher

Tags

His6 tag

Expression

Bacterial

Mutation

changed G842T and D848G of S. pneumoniae adhesin …

Promoter

T7

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCMVTAG-NEMO

Plasmid

#11970

Depositor

Jon Ashwell

Article

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMO (IKBKG Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only

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