We narrowed to 8,814 results for: Ott
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Plasmid#231588PurposeALPK1 gene mutated in position V1092A (mutation found in spiradenomacarcinoma (sweat gland cancer)) under control of a doxycycline-inducible promoterDepositorInsertALPK1 (ALPK1 Human)
UseLentiviralTags3X flagExpressionMammalianMutationchanged Valine1092 to AlaninePromoterdoxycyclineAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 ALPK1 T237M
Plasmid#231586PurposeALPK1 gene mutated in position T237M (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoterDepositorInsertALPK1 (ALPK1 Human)
UseLentiviralTags3X flagExpressionMammalianMutationchanged threonine 237 to MethioninePromoterdoxycyclineAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Ribo-jGCaMP8m
Plasmid#167574PurposeDEPRECATED: Please see Addgene item #169248 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m.DepositorHas ServiceAAV1InsertRibo-GCaMP8m
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
AT5G63930 X059_pECIA2
Plasmid#115116PurposeBait vector AT5G63930 X059_pECIA2 should be used with prey vector AT5G63930 X059_pECIA14.DepositorInsertAT5G63930 (AT5G63930 Mustard Weed)
UseTagsFc, V5 and hexahistidine tagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT1G17230 X074_pECIA2
Plasmid#115096PurposeBait vector AT1G17230 X074_pECIA2 should be used with prey vector AT1G17230 X074_pECIA14.DepositorInsertAT1G17230 (AT1G17230 Mustard Weed)
UseTagsFc, V5 and hexahistidine tagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT2G33170 X049_pECIA2
Plasmid#115101PurposeBait vector AT2G33170 X049_pECIA2 should be used with prey vector AT2G33170 X049_pECIA14.DepositorInsertAT2G33170 (AT2G33170 Mustard Weed)
UseTagsFc, V5 and hexahistidine tagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT1G74360 X038_pECIA2
Plasmid#115080PurposeBait vector AT1G74360 X038_pECIA2 should be used with prey vector AT1G74360 X038_pECIA14.DepositorInsertAT1G74360 (AT1G74360 Mustard Weed)
UseTagsFc, V5 and hexahistidine tagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT5G63930 X059_pECIA14
Plasmid#114916PurposePrey vector AT5G63930 X059_pECIA14 should be used with bait vector AT5G63930 X059_pECIA2.DepositorInsertAT5G63930 (AT5G63930 Mustard Weed)
UseTagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT1G17230 X074_pECIA14
Plasmid#114896PurposePrey vector AT1G17230 X074_pECIA14 should be used with bait vector AT1G17230 X074_pECIA2.DepositorInsertAT1G17230 (AT1G17230 Mustard Weed)
UseTagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT2G33170 X049_pECIA14
Plasmid#114901PurposePrey vector AT2G33170 X049_pECIA14 should be used with bait vector AT2G33170 X049_pECIA2.DepositorInsertAT2G33170 (AT2G33170 Mustard Weed)
UseTagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT1G74360 X038_pECIA14
Plasmid#114880PurposePrey vector AT1G74360 X038_pECIA14 should be used with bait vector AT1G74360 X038_pECIA2.DepositorInsertAT1G74360 (AT1G74360 Mustard Weed)
UseTagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS D614 soluble Spike
Plasmid#164652PurposeExpresses SARS-CoV-2 soluble Spike proteinDepositorInsertSARS-CoV-2 soluble spike (S SARS-CoV-2)
UseTagsHISExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-ataxin-3 C14A
Plasmid#89977PurposeExpresses catalytic inactive GFP-tagged ataxin-3DepositorInsertataxin-3 (ATXN3 Human)
UseTagsGFPExpressionMammalianMutationchanged Cysteine 14 to AlaninePromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP1-N1-Dsup-C
Plasmid#90022PurposeTo express GFP-tagged C-terminal region of Dsup in mammalian cells transientlyDepositorInsertDsup
UseTagsAcGFP1ExpressionMammalianMutationC-terminal region alonePromoterCMVAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-FLPo-Myc
Plasmid#124641PurposeAAV-mediated Cre-dependent expression of FLPo recombinase under EF1a promoterDepositorInsertFLPo
UseAAV and Cre/LoxTagsmyc tagExpressionMammalianMutationPromoterEF1aAvailable sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9-PGK-Puro-IRES-GFP
Plasmid#75114PurposeRetroviral expression plasmid encoding wild type human BRD9DepositorInsertBRD9 (BRD9 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - EGFP sgRNA 3
Plasmid#51762PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
EGFP sgRNA 3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFS and U6Available sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-mCherry-IRESMygro
Plasmid#82505PurposeVector targeting the mCherry gene to the GAPDH locus of human cells. mCherry is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.DepositorInsertmCherry-IRESMygro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK mKate2-OGT D554N (Puro)
Plasmid#154292PurposeLentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, D554N variant, no Ser/Thr glycosylation)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x FLAG and mKate2ExpressionMammalianMutationD554N mutation (Ser/Thr glycosylation-incompetent)PromoterPGKAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FDIO-ArchT-GFP
Plasmid#124640PurposeAAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoterDepositorInsertArchT
UseAAV; Flp/frtTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT4G23740 J11_pECIA2
Plasmid#114998PurposeBait vector AT4G23740 J11_pECIA2 should be used with prey vector AT4G23740 J11_pECIA14.DepositorInsertAT4G23740 (AT4G23740 Mustard Weed)
UseTagsFc, V5 and hexahistidine tagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-eGFP-IRESMygro
Plasmid#82503PurposeVector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.DepositorInserteGFP-IRESMygro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTBL210
Plasmid#126451PurposeExpresses Cas9-XTEN-TdT in mammalian cells.DepositorInsertCas9-XTEN-TdT (DNTT Human, Streptococcus pyogenes)
UseTagsExpressionMammalianMutationSee depositor comments belowPromoterCMVAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Ribo-jGCaMP8f
Plasmid#167573PurposeDEPRECATED: Please see Addgene item #169249 (coming soon) for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f.DepositorInsertRibo-GCaMP8f
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_dBD-PGK-Puro-IRES-GFP
Plasmid#75115PurposeRetroviral expression plasmid encoding a human BRD9 mutant lacking the bromodomainDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTagsExpressionMutationdeleted bromodomainPromoterAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRosa26-EF1a-hCas9IRESneo
Plasmid#67987PurposeMouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassetteDepositorInsertEF1a-hCas9IRESneopA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Cterm_3xFL_BRD9-PGK-Puro-IRES-GFP
Plasmid#75122PurposeRetroviral expression plasmid encoding wild type human BRD9 with a C terminal 3x FLAG-tagDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTags3x FLAG-tagExpressionMutationPromoterAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-WT_RASA2-EF1-Puro
Plasmid#114096PurposeExpress RASA2 in mamalian cellsDepositorInsertRASA2 (RASA2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHIV-ARF-mOrange2
Plasmid#110731PurposeMammalian Expression of p14ARF IRES H2B-mOrange2DepositorInsertp14ARF IRES H2B-mOrange2 (CDKN2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro CCND2-T2A-luciferase-P2A-eGFP
Plasmid#179843PurposeTunable and temporal expression control of CCND2, luciferase and nuclear eGFPDepositorInsertCCND2 (CCND2 Human)
UseLuciferase and Synthetic BiologyTagseGFP and luciferaseExpressionMammalianMutationPromoterTRE3GAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 SARS-CoV-2 S D614G-trunc
Plasmid#158077PurposeExpresses SARS-CoV-2 spike protein with a D614G truncated mutationDepositorInsertSARS-CoV-2 spike (S )
UseTagsExpressionMammalianMutationD614GPromoterAvailable sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-CBA-hGBA1-cm-hGBA1-hGHpA
Plasmid#218795PurposeAAV reporter genome plasmidDepositorInsertCMV-CBA-hGBA1-cm-hGBA1-hGHpA
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEMS1424
Plasmid#29220PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertPle155-EGFP-NLS
UseHigh copy number homologous recombination plasmid…TagsExpressionMammalianMutationPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS2242
Plasmid#111897PurposessAAV genome with Ple321 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertPle321 (TUBB3 Human)
UseAAVTagsExpressionMutationPromoterPle321 (TUBB3 MiniPromoter)Available sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
YA1627: pAAV_hSyn-Dio-paQuasAr3-s-P2A-CheRiff-s
Plasmid#107704Purposein vivo voltage imagingDepositorInsertpaQuasAr3-P2A-CheRiff
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK mKate2-OGT 5N5A (Puro)
Plasmid#154293PurposeLentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, 5N5A variant, no HCF-1 cleavage)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x FLAG and mKate2ExpressionMammalianMutationN322A, N356A, N390A, N424A, N458A mutations; HCF-…PromoterPGKAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6-sgfiller-eCas9-T2A-BlastR
Plasmid#172437PurposeExpresses eCas9, BlastR and sgRNA cloning siteDepositorInsertseCas9
BlastR
UseCRISPR, Lentiviral, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mouse Gfi1-IRES-GFP
Plasmid#91891PurposeRetroviral expression of mouse Gfi1DepositorInsertGfi1 (Gfi1 Mouse)
UseRetroviralTagsIRES-EGFPExpressionMammalianMutationPromoterMMLV gagAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C1-Eps8 ∆cap
Plasmid#74786Purposemammalian expression of the capping activity mutant Eps8 fused to GFPDepositorInsertEps8 (Eps8 Mouse)
UseTagsExpressionMammalianMutationV689D, L693DPromoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Myc-PGK-Puro-IRES-GFP
Plasmid#75124PurposeRetroviral expression plasmid encoding wild type murine MycDepositorInsertmyelocytomatosis oncogene (Myc Mouse)
UseRetroviralTagsNoneExpressionMutationPromoterAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - EGFP sgRNA 6
Plasmid#51765PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
EGFP sgRNA 6
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFS and U6Available sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
SITS-MmCav1-EGFP-APEX2
Plasmid#170525PurposeVector expression of MmCav1-EGFP-APEX2 in Leishmania cell free system. Parton lab clone JEJDepositorInsertCaveolin1 (Cav1 Mouse)
UseSynthetic BiologyTagsAPEX2ExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)
Plasmid#26797DepositorInsertEF1a-tTA/TetO-mCherry-P2A-Rs1 (HTR4 Human)
UseTagsExpressionMammalianMutationHuman 5HT4B receptor with D100A mutation, generat…PromoterAvailable sinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 SARS-CoV-2 S D614-trunc
Plasmid#158076PurposeExpresses SARS-CoV-2 spike protein with a truncationDepositorInsertSARS-CoV-2 spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.2EmGFP-cDAXX-0SB
Plasmid#169728PurposeMammalian expression construct of GFP tagged cDAXX-0SBDepositorInsertcDAXX-0SB (DAXX Human)
UseTagsGFPExpressionMammalianMutationComprises residues 495-741 and has the major SB m…PromoterCMVAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C1-Eps8 ∆bund
Plasmid#74889Purposemammalian expression of the bundling activity mutant Eps8 fused to GFPDepositorInsertEps8 (Eps8 Mouse)
UseTagsExpressionMammalianMutationL757A, K759APromoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS2274
Plasmid#111900PurposessAAV genome with Ple344 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertPle344 (TUBB3 Human)
UseAAVTagsExpressionMutationPromoterPle344 (TUBB3 MiniPromoter)Available sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEMS1062
Plasmid#29011PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertPle155-Cre/EGFP-NLS
UseHigh copy number homologous recombination plasmid…TagsExpressionMammalianMutationPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
tdEos-LaminB1-10
Plasmid#57632PurposeLocalization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertLaminB1 (LMNB1 Human)
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTBL469
Plasmid#126458PurposeExpresses Cas9-15xFlag-TdT in mammalian cells.DepositorInsertsUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only