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HEK3_pegF_18bp

Plasmid

#174852

Purpose

Express forward pegRNA mediating 991-bp deletion and 18-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegF_18bp insertion

Expression

Mammalian

Promoter

U6

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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HEK3_pegR_18bp

Plasmid

#174853

Purpose

Express reverse pegRNA mediating 991-bp deletion and 18-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegR_18bp

Expression

Mammalian

Promoter

U6

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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HEK3_pegF_44bp

Plasmid

#174854

Purpose

Express forward pegRNA mediating 991-bp deletion and 44-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegF_44bp insertion

Expression

Mammalian

Promoter

U6

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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HEK3_pegR_44bp

Plasmid

#174855

Purpose

Express forward pegRNA mediating 991-bp deletion and 44-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegR_44bp insertion

Expression

Mammalian

Promoter

U6

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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JBEI-9348

Plasmid

#84981

Purpose

Isopentenol production

Depositor

Taek Soon Lee

Article

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Insert

PMD-NudB

Expression

Bacterial

Promoter

trc promoter

Available Since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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JBEI-9314

Plasmid

#84979

Purpose

Isopentenol production

Depositor

Taek Soon Lee

Article

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Insert

PMD

Expression

Bacterial

Promoter

trc promoter

Available Since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pUASp YFP Rabx5 WT

Plasmid

#53494

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rabx5

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rabx2 DN

Plasmid

#53475

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rabx2

Tags

YFP

Expression

Insect

Mutation

S21N

Promoter

GAL4

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-hIBA1a-GFP-miR124T

Plasmid

#214147

Purpose

AAV vector to restrict GFP expression in microglia.

Depositor

Chun-Li Zhang

Article

Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

Has Service

AAV5

Insert

GFP

Use

AAV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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scAAV-hIBA1a-GFP

Plasmid

#214146

Purpose

Self-complementary AAV vector packaging GFP under the human Iba1 promoter

Depositor

Chun-Li Zhang

Article

Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

Insert

GFP

Use

AAV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCas9

Plasmid

#42876

Purpose

Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide

Depositor

Luciano Marraffini

Article

Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

tracr/Cas9

Use

CRISPR; E.coli

Expression

Bacterial

Available Since

Feb. 26, 2013

Availability

Academic Institutions and Nonprofits only

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pHCMV_Bless

Plasmid

#207255

Purpose

Mammalian expression of Baboon Endogenous Retrovirus env protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

BaboonEndogenousRetrovirus_Rless_env

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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PB-CRISPR

Plasmid

#160047

Purpose

For cloning sgRNA to knockout a specific gene in piggybac plasmid

Depositor

Xiaojun Lian

Article

Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

Type

Empty backbone

Use

CRISPR; Piggybac

Expression

Mammalian

Promoter

EFS

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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T4 td upstream intron-PABP

Plasmid

#189972

Purpose

Part 1 plasmid to introduce the T4 td upstream intron and PABP spacer into circRNAs

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

T4 td upstream intron-PABP

Use

Cloning

Promoter

N/A

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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mRNA-CleanCap-mNeonGreen

Plasmid

#188248

Purpose

Template plasmid to transcribe mRNA encoding mNeonGreen

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Expression

Mammalian

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTF101.1gw1

Plasmid

#134768

Purpose

A binary plasmid for gateway 1-Entry in pTF101.1 background (attR1 & attR2) containing double CaMV 35S promoter and TEV enhancer to drive bialaphos resistance gene (bar) for plant transformation

Depositor

Kan Wang

Article

et al Euphytica (2004) 136: 167.

Type

Empty backbone

Expression

Plant

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pH2B-miRFP713-N1

Plasmid

#197230

Purpose

Expresses the fusion protein of H2B-miRFP713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

H2B-miRFP713

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_SFV_E1E2

Plasmid

#207308

Purpose

Mammalian expression of Semliki Forest virus E1E2 protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

SFV_E1E2

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_COCVmut_G

Plasmid

#207262

Purpose

Mammalian expression of a mutant Cocal virus G protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

COCVmut_G

Expression

Mammalian

Mutation

K47A/Y209A/R354A; mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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mito-EcSTH-LbNOX

Plasmid

#211922

Purpose

Mitochondrial localization of EcSTH-LbNOX

Depositor

Binghui Li

Article

Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

Insert

mito-EcSTH-LbNOX

Use

Lentiviral

Tags

3xFLAG

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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Skylan-S-N1

Plasmid

#129737

Purpose

A green photo-switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI)

Depositor

Pingyong Xu

Article

Zhang et al ACS Nano. 2015 Mar 24;9(3):2659-67. doi:

Type

Empty backbone

Tags

Skylan-S

Expression

Mammalian

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTW335l

Plasmid

#246590

Purpose

G. hirsutum Rubisco gene

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

G. hirsutum RbcL, RbcS 6xhis (rbcL G. hirsutum RbcL)

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHCMV_GP64

Plasmid

#207276

Purpose

Mammalian expression of baculovirus GP64 protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

GP64

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCAP03-acc(3)IV

Plasmid

#69862

Purpose

pCAP03-acc(3)IV is a upgraded version of the pCAP01(Plasmid #59981)

Depositor

Bradley Moore

Article

Tang et al ACS Chem Biol. 2015 Oct 21.

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Nov. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pHCMV_FLUAV_HA

Plasmid

#207273

Purpose

Mammalian expression of Influenza A virus HA protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

FLUAV_HA (HA Influenza A virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pH2B-miRFP680-N1

Plasmid

#197229

Purpose

Expresses the fusion protein of H2B-miRFP680 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

H2B-miRFP680

Expression

Mammalian

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miRFP720-HO1

Plasmid

#197257

Purpose

Expresses the protein of miRFP720 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP720

Expression

Bacterial

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR::rpsL

Plasmid

#44505

Purpose

A crRNA expression plasmid specific to the rpsL allele.

Depositor

Luciano Marraffini

Article

Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

CRISPR::rpsL (rpsL )

Use

CRISPR; E.coli

Available Since

April 16, 2013

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-emiRFP670-HO1

Plasmid

#197252

Purpose

Expresses the protein of emiRFP670 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

emiRFP670

Expression

Bacterial

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
EK0329 CMV-TO-FRB-MCP (FLP-IN)

Plasmid

#233375

Purpose

FRB fused with MCP

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FRB-tdMCP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-HisD-miRFP680-HO1

Plasmid

#197253

Purpose

Expresses the protein of miRFP680 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP680

Expression

Bacterial

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-mRhubarb720

Plasmid

#197212

Purpose

Expresses the protein of mRhubarb720 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mRhubarb720

Use

AAV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCMV_BoDV1_G

Plasmid

#207256

Purpose

Mammalian expression of Borna disease virus 1 G protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

BoDV1_G

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCB-hDot1Lwt

Plasmid

#74173

Purpose

For packaging hDot1Lwt to retrovirus.

Depositor

Yi Zhang

Article

Okada et al Cell. 2005 Apr 22;121(2):167-78.

Insert

hDot1L (DOT1L Human)

Use

Retroviral

Tags

HA

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN-sacB

Plasmid

#196249

Purpose

Plasmid containing the sacB gene

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

attR2-sacB-attL3

Expression

Bacterial

Promoter

sacB promoter

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBB-XIAP

Plasmid

#11558

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAP (XIAP Human)

Tags

Flag

Expression

Mammalian

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-rev-erbα

Plasmid

#186823

Purpose

Fluorescent reporter for rev-erbα expression

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

rev-erbα promoter

Use

Luciferase

Promoter

rev-erbα promoter

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
3xFLAG-NanoLuc

Plasmid

#189975

Purpose

Part 3-4-5 plasmid to introduce 3xFLAG-NanoLuc into circRNAs

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

NanoLuc

Use

Cloning

Tags

3xFLAG

Promoter

N/A

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCMV-VSVgSS-SNAP-TM

Plasmid

#207319

Purpose

Functional incorporation of SNAP-tag into envelope of virions

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

SNAP-tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TwinStrepSUMO-pvc10-Cas9

Plasmid

#243755

Purpose

For affinity purification of Pvc10 fused to Cas9

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc10-Cas9

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAWP78-PVCpnf_pvc13-SpyTag_Δpvc10

Plasmid

#243740

Purpose

PVC structural operon - tail fiber (Pvc13) tagged with SpyTag - deleted spike tip (Pvc10)

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-SpyTag_Δpvc10

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCBL101

Plasmid

#199722

Purpose

A pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene.

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

NPTII

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b-Rhobin2

Plasmid

#239018

Purpose

Bacterial overexpression of de novo Rhodamine binder 2 (Rhobin2) protein

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

RhoBin2

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCMV_EBOV_GP

Plasmid

#207271

Purpose

Mammalian expression of Ebola virus GP protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

EBOV_GP (GP Ebola virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
K-SPOTIT1.0

Plasmid

#171215

Purpose

Expresses a kappa-opioid receptor-based circular permuted green fluorescent protein sensor

Depositor

Wenjing Wang

Article

Kroning et al Angew Chem Int Ed Engl. 2021 Mar 4. doi:

Insert

K-SPOTIT1.0

Use

Lentiviral

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCMV_HCV_E1E2

Plasmid

#207279

Purpose

Mammalian expression of Hepatitis C virus E1E2 protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

HCV_E1E2

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCBL102-RUBY

Plasmid

#210379

Purpose

Expresses betalain biosynthesis genes under CaMV 35S promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

35S-RUBY

Expression

Plant

Available Since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1/hChR2-EYFP

Plasmid

#20939

Purpose

Mammalian expression of humanized ChR2 fused to EYFP

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCMV_OROV_GP

Plasmid

#207297

Purpose

Mammalian expression of Oropouche virus GP protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

OROV_GP (M Oropouche virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCnCas12f1HS_sgRNA_MS13_empty

Plasmid

#204999

Purpose

CnCas12f1 with MS13 sgRNA site for Human cell genome editing

Depositor

Quanjiang Ji

Article

Su et al Nat Chem Biol. 2024 Feb;20(2):180-189. d

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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